Modelling the kinetics of thermal inactivation of apple polyphenoloxidase

The enzymatic browning of fruits and vegetables caused by mechanical injury during postharvest storage or processing is initiated by the catalytic action of polyphenoloxidase (PPO). A bleaching treatment prior to processing is still considered mostly effective in inhibiting the catalytic activity of PPO, and thus controlling undesirable enzymatic browning. In this work, different mathematical routines were assessed in terms of their adequacy to describe the thermal inactivation of PPO from Golden apples over a range of temperatures from 62.5 to 72.5 ºC. The classical approach to kinetic modelling of the decay activity of apple PPO, commonly reported to follow a first-order model, employs a two-step procedure, in which the model parameters are individually obtained, by each temperature studied, using non-linear or linear regressions. Thereafter, the estimated parameters are further used to calculate their temperature dependence. Alternatively, a one-step method provides a regression fit to all experimental data sets, with the temperature dependence equation being directly built in the kinetic model. This fitting technique thus, (a) avoids the estimation of intermediate parameters and, (b) substantially increases the degrees of freedom and hence the precision of parameters’ estimates. Within this issue was further explored the logarithmic transformation of the mathematical equations used on the adequacy of the model to describe experimental data. In all cases non-weighted least-squares regression procedures were used. Both the examination and criticism of the current modelling strategies were done by assessing statistical data obtained, such as the confidence intervals of the estimates, correlation coefficients, sum of squares, and residuals normality

Quantum signatures in quadratic optomechanics

We analyze quantum effects occurring in optomechanical systems where the coupling between an optical mode and a mechanical mode is quadratic in displacement (membrane-in-the-middle geometry). We show that it is possible to observe quantum effects in these systems without achieving the single-photon strong coupling regime. We find that zero-point energy causes a mechanical frequency shift, and we propose an experimental way to measure it. Further, we show that it is possible to determine the phonon statistics from the cavity transmission, and propose a way to infer the resonator's temperature based on this feature. For completeness, we revisit the case of an isolated system and show that different types of mechanical quantum states can be created, depending on the initial cavity state. In this situation, mechanical motion undergoes collapse and revivals, and we compute the collapse and revival times, as well as the degree of squeezing.Comment: 7 pages, 6 figures, 2nd versio

Resolvent estimates for the magnetic Schr\"odinger operator in dimension n≥2n \geq 2

It is well known that the resolvent of the free Schr\"odinger operator on weighted $L^2$ spaces has norm decaying like $\lambda^{-\frac{1}{2}}$ at energy $\lambda$. There are several works proving analogous high-frequency estimates for magnetic Schr\"odinger operators, with large long or short range potentials, in dimensions $n \geq 3$. We prove that the same estimates remain valid in all dimensions $n \geq 2$.Comment: 21 page