Synergistic antibacterial activity of monoterpenes in combination with conventional antimicrobials against Gram-positive and Gram-negative bacteria

Introduction: bacterial infections are a public health problem. Besides, the emergence of strains resistant to antimicrobials hascontributed to the search for new alternatives, such for the terpenes with antimicrobial potential. Objectives: the objective of thisstudy was to determine the possible interaction of isolated monoterpenes (-)-Carveol, Geraniol, Citronellol, α-terpineol, R-(-) Carvone,(-)-Menthol, Linalool, D-Dihydrocarvone, and (-)-Terpine-4-ol with conventional antimicrobials (Chloramphenicol, Minocycline,Amoxicillin and Ciprofloxacin) when they are evaluated on Staphylococcus aureus, Staphylococcus epidermidis, Bacillus subtilis,Escherichia coli and Pseudomonas aeruginosa strains. Methodology: the minimum inhibitory concentrations of these test drugswere determined using the microdilution method. The Checkerboard method was used to assess the interactions, by determiningthe fractional inhibitory concentration index (FIC index). Results: aamong the monoterpenes, only Carveol, Citronellol, and Geraniolpresented antimicrobial activity (MIC < 1024 μg/mL). They presented synergistic effects against Pseudomonas aeruginosa ATCC-9027(FIC index ≤ 0.5) when in combination with Minocycline. Conclusion: this study contributes to the development of new approachesto control bacterial resistance and to the possibility of discovering new drugs