505 research outputs found
The effect of hypobaric hypoxia on misonidazole binding in normal and tumour-bearing mice.
The effect of hypobaric hypoxia on the in vivo binding of misonidazole was investigated in normal mice and mice bearing T50/80 or CA NT mammary carcinomas. After the intraperitoneal injection of radiolabelled misonidazole, mice were randomised to breathe either room air or air at 0.5 atmospheres. The distribution of misonidazole in liver, kidney, heart, spleen and tumour tissue, 24 h later, was studied by scintillation counting and by autoradiography. Significantly higher misonidazole binding occurred in the livers (x2.5), kidneys (x2.4), spleens (x2.9) and hearts (x1.8) of hypoxic mice compared to controls. Hypobaric hypoxia was associated with a greater than four-fold increase in misonidazole binding within T50/80 tumours. However, significantly higher binding was not demonstrated within CA NT tumours after exposure of tumour-bearing animals to hypoxic conditions. In autoradiographs of hypoxic liver, labelling was intense in regions near to hepatic veins but sparse in areas surrounding portal tracts. This pattern was striking and consistent. In hypoxic kidney, labelling was most intense over tubular cells, less intense over glomeruli and sparse in the renal medulla. It is likely that the hepatic and renal cortical distributions of misonidazole binding reflect local oxygen gradients
Comparison of metal ion-induced conformational changes in parvalbumin and oncomodulin as probed by the intrinsic fluorescence of tryptophan 102.
The calcium-induced conformational changes of the 108-amino acid residue proteins, cod III parvalbumin and oncomodulin, were compared using tryptophan as a sensitive spectroscopic probe. As native oncomodulin is devoid of tryptophan, site-specific mutagenesis was performed to create a mutant protein in which tryptophan was placed in the identical position (residue 102) as the single tryptophan residue in cod III parvalbumin. The results showed that in the region probed by tryptophan-102, cod III parvalbumin experienced significantly greater changes in conformation upon decalcification compared to the oncomodulin mutant, F102W. Addition of 1 eq of Ca2+ produced greater than 90% of the total fluorescence response in F102W, while in cod III parvalbumin, only 74% of the total was observed. Cod III parvalbumin displayed a negligible response upon Mg2+ addition. In contrast, F102W did respond to Mg2+, but the response was considerably less when compared to Ca2+ addition. Time-resolved fluorescence showed that the tryptophan in both proteins existed in at least two conformational states in the presence of Ca2+ and at least three conformational states in its absence. Comparison with quantum yield measurements indicated that the local electronic environment of the tryptophan was significantly different in the two proteins. Collectively, these results demonstrate that both cod III parvalbumin and oncomodulin undergo Ca2(+)-specific conformational changes. However, oncomodulin is distinct from cod III parvalbumin in terms of the electronic environment of the hydrophobic core, the magnitude of the Ca2(+)-induced conformational changes, and the number of calcium ions required to modulate the major conformational changes
Angular dependent vortex pinning mechanisms in YBCO coated conductors and thin films
We present a comparative study of the angular dependent critical current
density in YBa2Cu3O7 films deposited on IBAD MgO and on single crystal MgO and
SrTiO3 substrates. We identify three angular regimes where pinning is dominated
by different types of correlated and uncorrelated defects. We show that those
regimes are present in all cases, indicating that the pinning mechanisms are
the same, but their extension and characteristics are sample dependent,
reflecting the quantitative differences in texture and defect density. In
particular, the more defective nature of the films on IBAD turns into an
advantage as it results in stronger vortex pinning, demonstrating that the
critical current density of the films on single crystals is not an upper limit
for the performance of the IBAD coated conductors.Comment: 14 pages, 3 figures. Submitted to AP
Normal state properties of high angle grain boundaries in (Y,Ca)Ba2Cu3O7-delta
By lithographically fabricating an optimised Wheatstone bridge geometry, we
have been able to make accurate measurements of the resistance of grain
boundaries in Y1-xCaxBa2Cu3O7-d between the superconducting transition
temperature, Tc, and room temperature. Below Tc the normal state properties
were assessed by applying sufficiently high currents. The behaviour of the
grain boundary resistance versus temperature and of the conductance versus
voltage are discussed in the framework charge transport through a tunnel
barrier. The influence of misorientation angle, oxygen content, and calcium
doping on the normal state properties is related to changes of the height and
shape of the grain boundary potential barrier.Comment: 17 pages, 1 table, 5 figures, submitted to PR
Depairing critical current achieved in superconducting thin films with through-thickness arrays of artificial pinning centers
Large area arrays of through-thickness nanoscale pores have been milled into
superconducting Nb thin films via a process utilizing anodized aluminum oxide
thin film templates. These pores act as artificial flux pinning centers,
increasing the superconducting critical current, Jc, of the Nb films. By
optimizing the process conditions including anodization time, pore size and
milling time, Jc values approaching and in some cases matching the
Ginzburg-Landau depairing current of 30 MA/cm^2 at 5 K have been achieved - a
Jc enhancement over as-deposited films of more than 50 times. In the field
dependence of Jc, a matching field corresponding to the areal pore density has
also been clearly observed. The effect of back-filling the pores with magnetic
material has then been investigated. While back-filling with Co has been
successfully achieved, the effect of the magnetic material on Jc has been found
to be largely detrimental compared to voids, although a distinct influence of
the magnetic material in producing a hysteretic Jc versus applied field
behavior has been observed. This behavior has been tested for compatibility
with currently proposed models of magnetic pinning and found to be most closely
explained by a model describing the magnetic attraction between the flux
vortices and the magnetic inclusions.Comment: 9 pages, 10 figure
Induced magnetization in LaSrMnO/BiFeO superlattices
Using polarized neutron reflectometry (PNR), we observe an induced
magnetization of 75 25 kA/m at 10 K in a LaSrMnO
(LSMO)/BiFeO superlattice extending from the interface through several
atomic layers of the BiFeO (BFO). The induced magnetization in BFO is
explained by density functional theory, where the size of bandgap of BFO plays
an important role. Considering a classical exchange field between the LSMO and
BFO layers, we further show that magnetization is expected to extend throughout
the BFO, which provides a theoretical explanation for the results of the
neutron scattering experiment.Comment: 5 pages, 4 figures, with Supplemental Materials. To appear in
Physical Review Letter
- …