High-Speed, Photon Counting CCD Cameras for Astronomy

The design of electron multiplying CCD cameras require a very different approach from that appropriate for slow scan CCD operation. This paper describes the main problems in using electron multiplying CCDs for high-speed, photon counting applications in astronomy and how these may be substantially overcome. With careful design it is possible to operate the E2V Technologies L3CCDs at rates well in excess of that claimed by the manufacturer, and that levels of clock induced charge dramatically lower than those experienced with commercial cameras that need to operate at unity gain. Measurements of the performance of the E2V Technologies CCD201 operating at 26 MHz will be presented together with a guide to the effective reduction of clock induced charge levels. Examples of astronomical results obtained with our cameras are presented.Comment: 11 pages, 5 figure

High-resolution imaging and spectroscopy in the visible from large ground-based telescopes with natural guide stars

Near-diffraction limited imaging and spectroscopy in the visible on large (8-10 meter) class telescopes has proved to be beyond the capabilities of current adaptive optics technologies, even when using laser guide stars. The need for high resolution visible imaging in any part of the sky suggests that a rather different approach is needed. This paper describes the results of simulations, experiments and astronomical observations that show that a combination of low order adaptive optic correction using a 4-field curvature sensor and fast Lucky Imaging strategies with a photon counting CCD camera systems should deliver 20-25 milliarcsecond resolution in the visible with reference stars as faint as 18.5 magnitude in I band on large telescopes. Such an instrument may be used to feed an integral field spectrograph efficiently using configurations that will also be described.Comment: 8 pages, 3 figure

GravityCam: Higher Resolution Visible Wide-Field Imaging

The limits to the angular resolution achievable with conventional ground-based telescopes are unchanged over 70 years. Atmospheric turbulence limits image quality to typically ~1 arcsec in practice. We have developed a new concept of ground-based imaging instrument called GravityCam capable of delivering significantly sharper images from the ground than is normally possible without adaptive optics. The acquisition of visible images at high speed without significant noise penalty has been made possible by advances in optical and near IR imaging technologies. Images are recorded at high speed and then aligned before combination and can yield a 3-5 fold improvement in image resolution. Very wide survey fields are possible with widefield telescope optics. We describe GravityCam and detail its application to accelerate greatly the rate of detection of Earth size planets by gravitational microlensing. GravityCam will also improve substantially the quality of weak shear studies of dark matter distribution in distant clusters of galaxies. The microlensing survey will also provide a vast dataset for asteroseismology studies. In addition, GravityCam promises to generate a unique data set that will help us understand of the population of the Kuiper belt and possibly the Oort cloud.This is the author accepted manuscript. The final version is available from http://dx.doi.org/10.1117/12.223090

Photon Counting EMCCDs: New Opportunities for High Time Resolution Astrophysics

Electron Multiplying CCDs (EMCCDs) are used much less often than they might be because of the challenges they offer camera designers more comfortable with the design of slow-scan detector systems. However they offer an entirely new range of opportunities in astrophysical instrumentation. This paper will show some of the exciting new results obtained with these remarkable devices and talk about their potential in other areas of astrophysical application. We will then describe how they may be operated to give the very best performance at the lowest possible light levels. We will show that clock induced charge may be reduced to negligible levels and that, with care, devices may be clocked at significantly higher speeds than usually achieved. As an example of the advantages offered by these detectors we will show how a multi-detector EMCCD curvature wavefront sensor will revolutionise the sensitivity of adaptive optics instruments and been able to deliver the highest resolution images ever taken in the visible or the near infrared.Comment: 9 pages, 5 Figures; SPIE vol 8453, 201

Aspergillus fumigatus enhances elastase production in pseudomonas aeruginosaco-cultures

In the cystic fibrosis (CF) lung the presence of bacteria and fungi in the airways promotes an inflammatory response causing progressive lung damage, ultimately leading to high rates of morbidity and mortality. We hypothesized that polymicrobial interactions play an important role in promoting airway pathogenesis. We therefore examined the interplay between the most commonly isolated bacterial CF pathogen, Pseudomonas aeruginosa, and the most prevalent filamentous fungi, Aspergillus fumigatus, to test this. Co-culture experiments showed that in the presence of A. fumigatus the production of P. aeruginosa elastase was enhanced. This was confirmed by the presence of zones of clearance on Elastin-Congo Red (ECR) agar, which was identified as elastase by mass spectrometry. When P. aeruginosa were grown in a co-culture model with mature A. fumigatus biofilms, 60% of isolates produced significantly more elastase in the presence of the filamentous fungi than in its absence (P < .05). The expression of lasB also increased when P. aeruginosa isolates PA01 and PA14 were grown in co-culture with A. fumigatus. Supernatants from co-culture experiments were also significantly toxic to a human lung epithelial cell line (19–38% cell cytotoxicity) in comparison to supernatants from P. aeruginosa only cultures (P < .0001). Here we report that P. aeruginosa cytotoxic elastase is enhanced in the presence of the filamentous fungi A. fumigatus, suggesting that this may have a role to play in the damaging pathology associated with the lung tissue in this disease. This indicates that patients who have a co-colonisation with these two organisms may have a poorer prognosis