23 research outputs found
Lipid Anti-Lipid Antibody Responses Correlate with Disease Activity in Systemic Lupus Erythematosus
10.1371/journal.pone.0055639PLoS ONE82
Global assessment of dengue Virus-Specific CD4+ T cell responses in Dengue-Endemic areas
Background: Dengue is a major public health problem worldwide. Assessment of adaptive immunity is important to understanding immunopathology and to define correlates of protection against dengue virus (DENV). To enable global assessment of CD4+ T cell responses, we mapped HLA-DRB1-restricted DENV-specific CD4+ T cell epitopes in individuals previously exposed to DENV in the general population of the dengue-endemic region of Managua, Nicaragua.
Methods: HLA class II epitopes in the population of Managua were identified by an in vitro IFNγ ELISPOT assay. CD4+ T cells purified by magnetic bead negative selection were stimulated with HLA-matched epitope pools in the presence of autologous antigen-presenting cells, followed by pool deconvolution to identify specific epitopes. The epitopes identified in this study were combined with those previously identified in the DENV endemic region of Sri Lanka, to generate a “megapool” (MP) consisting of 180 peptides specifically designed to achieve balanced HLA and DENV serotype coverage. The DENV CD4MP180 was validated by intracellular cytokine staining assays.
Results: We detected responses directed against a total of 431 epitopes, representing all 4 DENV serotypes, restricted by 15 different HLA-DRB1 alleles. The responses were associated with a similar pattern of protein immunodominance, overall higher magnitude of responses, as compared to what was observed previously in the Sri Lanka region. Based on these epitope mapping studies, we designed a DENV CD4 MP180 with higher and more consistent coverage, which allowed the detection of CD4+ T cell DENV responses ex vivo in various cohorts of DENV exposed donors worldwide, including donors from Nicaragua, Brazil, Singapore, Sri Lanka, and U.S. domestic flavivirus-naïve subjects immunized with Tetravalent Dengue Live-Attenuated Vaccine (TV005). This broad reactivity reflects that the 21 HLA-DRB1 alleles analyzed in this and previous studies account for more than 80% of alleles present with a phenotypic frequency ≥5% worldwide, corresponding to 92% phenotypic coverage of the general population (i.e., 92% of individuals express at least one of these alleles).
Conclusion: The DENV CD4 MP180 can be utilized to measure ex vivo responses to DENV irrespective of geographical location
Immunology: making magic bullets.
10.1136/bmj.39044.641817.94BMJ (Clinical research ed.)334 Suppl
Targeting tumours by adoptive transfer of immune cells
10.1111/j.1440-1681.2006.04409.xClinical and Experimental Pharmacology and Physiology335-6569-57
Pleiotropic Effects of Blastocystis spp. Subtypes 4 and 7 on Ligand-Specific Toll-Like Receptor Signaling and NF-κB Activation in a Human Monocyte Cell Line
10.1371/journal.pone.0089036PLoS ONE92e8903
Structure and function: Heat shock proteins and adaptive immunity
Journal of Immunology17942035-2040JOIM
Identification of human CD4+T-cell epitopes on the VP1 capsid protein of enterovirus 71
Viral Immunology212215-22
Current views on the clinical relevance of blastocystis spp.
10.1007/s11908-009-0073-8Current Infectious Disease Reports12128-35CIDR
The molecular engineering of an anti-idiotypic antibody for pharmacokinetic analysis of a fully human anti-infective
10.1371/journal.pone.0145381PLoS ONE1012e014538