Scatter plot of shape differences in <i>Siganus fuscescens</i> following geometric morphometric analysis.

<p>Each point represents an individual. Circle color indicates nuclear genotype cluster or mtDNA lineage. Scatter plot of individual scores based on analysis of 22 landmarks: (A) Principal plan (PC1 x PC2) of principal components analysis where individuals are distinguished according to nuclear genotype cluster; (B) Principal plan (CV1 x CV2) of canonical variates analysis where individuals are distinguished according to mtDNA lineage; (C) Principal plan of canonical variates analysis where individuals are distinguished according to nuclear genotype clusters; (D) Thin-plate spline deformation grid for the average Cluster 1 individual relative to the consensus from the total sample; pixel color ranges show compression (dark blue) to expansion (red); (E) Thin-plate spline deformation grid for the average Cluster 2A individual; (F) Thin-plate spline deformation grid for the average Cluster 2B individual.</p

Siganus fuscescens.

<p>Two-dimensional plot of correspondence analysis of individual genotypes at 12 microsatellite loci. Each point represents an individual (total <i>N</i> = 343). Colors indicate mtDNA control region lineages: Clade A (black), Clade B (white), Clade C (grey). Symbols indicate sampling region: Philippines (triangles) and Hong Kong (circles). Putative hybrids as revealed by NEWHYBRIDS analysis are marked (‘h’).</p

Isolation-with-migration analysis of mtDNA control region sequences for three pairwise comparisons of nuclear genotype clusters (Cluster 1, Cluster 2A, Cluster 2B) in <i>Siganus fuscescens</i> from the northern Philippines and Hong Kong.

<p>Isolation-with-migration analysis of mtDNA control region sequences for three pairwise comparisons of nuclear genotype clusters (Cluster 1, Cluster 2A, Cluster 2B) in <i>Siganus fuscescens</i> from the northern Philippines and Hong Kong.</p

Comparison of genotype cluster assignment and mtDNA lineage membership for <i>Siganus fuscescens</i> individuals.

<p>Each individual is represented by a vertical bar where the apportion of ancestry is represented by segments of different colors corresponding to different mitochondrial lineages and nuclear genotype clusters. Individuals with mixed ancestry are indicated by different colors corresponding to the inferred proportion of ancestry in a particular group. (a) MtDNA lineage membership; (b) Assignment to nuclear genotype cluster based on correspondence analysis; (c) Individual ancestry coefficient (<i>q</i>) inferred from STRUCTURE analysis when <i>K</i> = 2; (d) Individual ancestry coefficient (<i>q</i>) inferred from STRUCTURE analysis when <i>K</i> = 3. Putative hybrids are marked (‘h’).</p

Geographical distribution of the number of mitonuclear combinations in <i>Siganus fuscescens</i> from the northern Philippines and Hong Kong.

<p>Geographical distribution of the number of mitonuclear combinations in <i>Siganus fuscescens</i> from the northern Philippines and Hong Kong.</p

Count of individual mitonuclear combinations for three mtDNA lineages and three nuclear genotypes (Cluster 1, Cluster 2, and admixed^a) in <i>Siganus fuscescens</i> samples from the northern Philippines and Hong Kong.

<p>Count of individual mitonuclear combinations for three mtDNA lineages and three nuclear genotypes (Cluster 1, Cluster 2, and admixed<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0193220#t002fn001" target="_blank">^a</a>) in <i>Siganus fuscescens</i> samples from the northern Philippines and Hong Kong.</p

Posterior probability densities for divergence time and migration rate for <i>Siganus fuscescens</i> mitochondrial lineages within each of three nuclear clusters.

<p>Probability densities were estimated under the isolation-with-migration model (IMa2; [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0193220#pone.0193220.ref063" target="_blank">63</a>]). (a) Estimated divergence times; (b, c, d). Pairwise estimates of population migration rate (2Nm) between nuclear clusters.</p

Estimates of genetic differentiation and distribution of allelic diversity at 12 microsatellite loci in <i>Siganus fuscescens</i> from the northern Philippines and Hong Kong.

<p>Estimates of genetic differentiation and distribution of allelic diversity at 12 microsatellite loci in <i>Siganus fuscescens</i> from the northern Philippines and Hong Kong.</p

Genetic diversity estimates at 12 microsatellite loci for three genotype clusters of <i>Siganus fuscescens</i> from the northern Philippines and Hong Kong.

<p>Genetic diversity estimates at 12 microsatellite loci for three genotype clusters of <i>Siganus fuscescens</i> from the northern Philippines and Hong Kong.</p

Distribution of <i>Siganus fuscescens</i> genotype clusters and mtDNA lineages across sampling locations in the South China Sea and Philippine Sea.

<p>Circle size is proportional to sample size. (A) Genotype clusters; (B) MtDNA clades. Background map of the northern Philippines and surrounding regions was downloaded from Digital Vector Maps, San Diego (<a href="http://digital-vector-maps.com/" target="_blank">http://digital-vector-maps.com/</a>). Abbreviations for sampling locations: BOL, Bolinao; CRN, Coron; CUR, Currimao; HKG, Hong Kong; MAS, Masinloc; MOR, Morong; PAT, Patnanungan; SNF, San Fernando. (A) Genotype clusters; (B) MtDNA clades.</p