Characterization and taxonomic placement of Rhizoctonia--like endophytes from orchid roots

Twenty-one Rhizoctoniu-like fungal strains were isolated from the roots of four terrestrial orchid species from various locations in Hong Kong. The cultural morpholon, uclear number of the hyphal cell, pore ultrastructure, and RAPD and CAPS analyses of rDNA fragments revealed that nlost of these isolates Tvere associated with the genera Clatorlziza and Epulorhizn. W Dan alysis showed the presence of genetic diversity between the isolates froin different hosts and locations. The compatibility between a selection of these Ceratorhiza and Epulorhiza isolates and 14 orchld species was determined using a symbiotic gerrninatlon method The germinatioil and development of three orchld species, .Arundina chinensis, Spathoglottis poubescens, and Spiranthes honkongensis, were strongly stimulated by the Epulorhiza isolates. Habenaria dentata was found to form symbionts successfully with a Ceratorhiza isolate.published_or_final_versio

Investigation of the effect of thermal insulation materials on packaging performance

This investigation evaluates thermal insulation performance of a typical shipping container with different insulation materials. A mathematical model developed from our previous work was used to analyse the effect of packaging characteristics on insulative performance. A number of materials were employed as a liner to insulate a typical cardboard box and the effect of these materials on package insulative performance was evaluated through experimental tests and the transistent thermal model. The results showed that application of aluminium foil to the internal liner surface of polyethylene gave 46% increase in the package insulative performance compared to the original polyethylene insulated packages. An improvement of 79% and 106% in insulative performance per unit liner thickness was obtained from packages insulated with polyisocyanurate board and aerogel blanket compared to the polystyrene insulated package.The results also indicated that temperature surrounding the package played a significant role in the maximum insulation time. Furthermore, an excellent agreement was obtained between the mathematical model and the experimental results across all packaging aspects studied in this work

Computer games: A double-edged sword?

Excessive computer game playing (ECGP) has already become a serious social problem. However, limited data from experimental lab studies are available about the negative consequences of ECGP on players' cognitive characteristics. In the present study, we compared three groups of participants (current ECGP participants, previous ECGP participants, and control participants) on a Multiple Object Tracking (MOT) task. The previous ECGP participants performed significantly better than the control participants, which suggested a facilitation effect of computer games on visuospatial abilities. Moreover, the current ECGP participants performed significantly worse than the previous ECGP participants. This more important finding indicates that ECGP may be related to cognitive deficits. Implications of this study are discussed. © 2008 Mary Ann Liebert, Inc.published_or_final_versio

Micropollutants removal and health risk reduction in a water reclamation and ecological reuse system

© 2018 Elsevier Ltd As reclaimed water use is increasing, its safety attracts growing attention, particularly with respect to the health risks associated with the wide range of micropollutants found in the reclaimed water. In this study, sophisticated analysis was conducted for water samples from a water reclamation and ecological reuse system where domestic wastewater was treated using an anaerobic-anoxic-oxic unit followed by a membrane bioreactor (A2O-MBR), and the reclaimed water was used for replenishing a landscape lake. A total of 58 organic micropollutants were detected in the system, consisting of 13 polycyclic aromatic hydrocarbons (PAHs), 16 phenols, 3 pesticides, and 26 pharmaceuticals and personal care products (PPCPs). After treatment by the A2O-MBR process, effective removal of pesticides and phenols was achieved, while when the reclaimed water entered the landscape lake, PPCPs were further removed. From the physicochemical properties of micropollutants, it could be inferred that phenols and dichlorphos (the only pesticide with considerable concentration in the influent) would have been mainly removed by biodegradation and/or volatilization in the biological treatment process. Additionally, it is probable that sludge adsorption also contributed to the removal of dichlorphos. For the predominant PPCP removal in the landscape lake, various actions, such as adsorption, biodegradation, photolysis, and ecologically mediated processes (via aquatic plants and animals), would have played significant roles. However, according to their logKoc, logKow and logD (pH = 8) values, it could be concluded that adsorption by suspended solids might be an important action. Although carcinogenic and non-carcinogenic risks associated with all the detected micropollutants were at negligible levels, the hazard quotients (HQs) of PPCPs accounted for 92.03%–97.23% of the HQTotal. With the significant removal of PPCPs through the ecological processes in the landscape lake, the safety of reclaimed water use could be improved. Therefore, the introduction of ecological unit into the water reclamation and reuse system could be an effective measure for health risk reduction posed by micropollutants

Reverse osmosis pretreatment method for toxicity assessment of domestic wastewater using Vibrio qinghaiensis sp.-Q67

Luminescent bacterial test is a fast and sensitive method for acute toxicity assessment of water and wastewater. In this study, an improved toxicity testing method was developed using the freshwater luminescent bacteria Vibrio qinghaiensis sp.-Q67 that involved pretreatment of water samples with reverse osmosis (RO) to eliminate the interferences caused by nutrients in concentrated samples and to improve the reliability and sensitivity of the analysis. Because water samples contain low concentrations of several target toxic substances, rapid acute toxicity testing method that is commonly employed does not achieve enough sensitivity. The proposed RO pretreatment could effectively enrich organic and inorganic substances in water samples to enable a more effective and sensitive toxicity evaluation. The kinetic characteristics of toxicity of raw sewage and secondary effluent were evaluated based on the relative luminescence unit (RLU) curves and time-concentration-effect surfaces. It was observed that when the exposure time was prolonged to 8-h or longer, the bacteria reached the logarithmic growth stage. Hence, the stimulating effects of the coexisting ions (such as Na+, K+, NO3-) in the concentrated samples could be well eliminated. A 10-h exposure time in proposed Q67 test was found to quantitatively evaluate the toxicity of the organic and inorganic pollutants in the RO-concentrated samples. © 2013 Elsevier Inc

The treatability of trace organic pollutants in WWTP effluent and associated biotoxicity reduction by advanced treatment processes for effluent quality improvement

© 2019 Elsevier Ltd As increasing attention is paid to surface water protection, there has been demand for improvements of domestic wastewater treatment plant (WWTP) effluent. This has led to the application of many different advanced treatment processes (ATPs). In this study, the treatability of trace organic pollutants in secondary effluent (SE) and associated biotoxicity reduction by four types of ATPs, including coagulation, granular activated carbon (GAC) adsorption, ultraviolet (UV) photolysis and photocatalysis, and ozonation, were investigated at the bench-scale. The ATPs showed different removal capacity for the 48 chemicals, which were classified into seven categories. EDCs, herbicides, bactericides and pharmaceuticals were readily degraded, and insecticides, flame retardants, and UV filters were relatively resistant to removal. During these processes, the efficiency of the ATPs in reducing four biological effects were investigated. Of the four biological effects, the estrogenic activity from SE was not detected using the yeast estrogen screen. In contrast with genotoxicity and photosynthesis inhibition, bacterial cytotoxicity posed by SE was the most difficult biological effect to reduce with these ATPs. GAC adsorption and ozonation were the most robust treatment processes for reducing the three detected biotoxicities. UV photolysis and photocatalysis showed comparable efficiencies for the reduction of genotoxicity and photosynthesis inhibition. However, coagulation only performed well in genotoxicity reduction. The effect-based trigger values for the four bioassays, that were derived from the existing environmental quality standards and from HC5 (hazardous concentration for 5% of aquatic organisms), were all used to select and optimize these ATPs for ecological safety. Conducting ATPs in more appropriate ways could eliminate the negative effects of WWTP effluent on receiving water bodies

Assessment of multiple hormone activities of a UV-filter (octocrylene) in zebrafish (Danio rerio)

© 2016 Elsevier Ltd In this study, zebrafish (Danio rerio) were exposed to a UV-filter-octocrylene (OCT) with elevated concentrations for 28 d. The total body accumulation of OCT in zebrafish was found to reach 2321.01 (“L” level), 31,234.80 (“M” level), and 70,593.38 ng g−1 (“H” level) when the average OCT exposure concentration was controlled at 28.61, 505.62, and 1248.70 μg L−1, respectively. Gross and histological observations as well as RT-qPCR analysis were conducted to determine the effects of OCT accumulation on zebrafish. After exposure, the gonad-somatic index and percentage of vitellogenic oocytes were found to increase significantly in the ovaries of female zebrafish at the H accumulation level. Significant up-regulation of esr1 and cyp19b were observed in the gonads, as well as vtg1 in the livers for both female and male zebrafish. At M and H accumulation levels, apparent down-regulation of ar was observed in the ovaries and testis of the female and male zebrafish, respectively. Although the extent of the effects on zebrafish differed at different accumulation levels, the induction of vtg1 and histological changes in the ovaries are indications of estrogenic activity and the inhibition of esr1 and ar showed antiestrogenic and antiandrogenic activity, respectively. Thus, as OCT could easily accumulate in aquatic life such as zebrafish, one of its most of concern hazards would be the disturbance of the histological development and its multiple hormonal activities

Interplay between topological insulators and superconductors

Topological insulators are insulating in the bulk but possess metallic surface states protected by time-reversal symmetry. Here, we report on a detailed electronic transport study in high-quality Bi 2Se 3 topological insulator thin films contacted by superconducting (In, Al, and W) electrodes. The resistance of the film shows an abrupt and significant upturn when the electrodes become superconducting. In turn, the Bi 2Se 3 film greatly weakens the superconductivity of the electrodes, significantly reducing both their transition temperatures and their critical fields. A possible interpretation of these results is that the superconducting electrodes are accessing the surface states and the experimental results are consequences of the interplay between the Cooper pairs of the electrodes and the spin-polarized current of the surface states in Bi 2Se 3. © 2012 American Physical Society.published_or_final_versio

Day-ahead allocation of operation reserve in composite power systems with large-scale centralized wind farms

This paper focuses on the day-ahead allocation of operation reserve considering wind power prediction error and network transmission constraints in a composite power system. A two-level model that solves the allocation problem is presented. The upper model allocates operation reserve among subsystems from the economic point of view. In the upper model, transmission constraints of tielines are formulated to represent limited reserve support from the neighboring system due to wind power fluctuation. The lower model evaluates the system on the reserve schedule from the reliability point of view. In the lower model, the reliability evaluation of composite power system is performed by using Monte Carlo simulation in a multi-area system. Wind power prediction errors and tieline constraints are incorporated. The reserve requirements in the upper model are iteratively adjusted by the resulting reliability indices from the lower model. Thus, the reserve allocation is gradually optimized until the system achieves the balance between reliability and economy. A modified two-area reliability test system (RTS) is analyzed to demonstrate the validity of the method.This work was supported by National Natural Science Foundation of China (No. 51277141) and National High Technology Research and Development Program of China (863 Program) (No. 2011AA05A103)

Computationally Guided Design of a Readily Assembled Phosphite- Thioether Ligand for a Broad Range of Pd-Catalyzed Asymmetric Allylic Substitutions

A modular approach employing indene as common starting material, has enabled the straightforward preparation in three reaction steps of P-thioether ligands for the Pd-catalyzed asymmetric allylic substitution. The analysis of a starting library of P-thioether ligands based on rational design and theoretical calculations has led to the discovery of an optimized anthracenethiol derivative with excellent behavior in the reaction of choice. Improving most approaches reported to date, this ligand presents a broad substrate and nucleophile scope. Excellent enantioselectivities have been achieved for a range of linear and cyclic allylic substrates using a large number of C-, N-, and O-nucleophiles (40 compounds in total). The species responsible for the catalytic activity have been further investigated by NMR in order to clearly establish the origin of the enantioselectivity. The resulting products have been derivatized by means of ring-closing metathesis or Pauson–Khand reactions to further prove the synthetic versatility of the methodology for preparing enantiopure complex structures