Antioxidant effect of EGb

Aqueous solutions of human low-density lipoproteins (LDL) (3 g/l) buffered at pH 7 (sodium phosphate 10-2 M, sodium formate 10-1 M) saturated with O2 have been irradiated between 0 and 500 Gy (dose rate 2.4 × 10-2 Gy/s) in the presence of increasing concentrations of Ginkgo biloba extract (EGb761) (0, 15, 30, 50 and 100 μg/ml). The protective effect of EGb761 after the [math] free radical action on LDL, was monitored by several lipid peroxidation markers: 1/ decrease of endogenous vitamin E and β carotene, 2/ formation of thiobarbituric acid-reactive substances (TBARS), 3/ formation of conjugated dienes and 4/ appearance of differential fluorescence.LDL peroxidation is markedly decreased in the presence of EGb761, this effect being EGb761 concentration dependent

Effect of EGb

Human low-density lipoproteins (LDL) aqueous solutions (3 g/l, 10-2 M sodium phosphate buffer, pH 7) have been irradiated in the presence of Ginkgo biloba extract (EGb761) in order to determine the protective effect of EGb761 against lipid peroxidation. Oxy free radicals, OH• and [math], were simultaneously produced from water radiolysis and their action on LDL was followed by determining the vitamin E and β-carotene contents, the formation of TBARS (thiobarbituric acid reactive substances) and conjugated dienes. Two concentrations of EGb761 (15 and 30 μg/ml) have been studied, the better antioxidant effect being obtained for the highest EGb761 concentration