Genetic variability of Artemisia capillaris (Wormwood capillary) by random amplified polymorphic DNA (RAPD) in Terengganu State, Malaysia

The genetic variability among individuals of Artemisia capillaris from state of Terengganu, Malaysia was examined by using the random amplified polymorphic DNA (RAPD) technique. The samples were collected from differences regional in Terengganu State. The genomic DNA was extracted from the samples leaves. Fifthty-seven oligonucleotide primers were screened and ten primers were selected (OPA 04, OPA 09, OPA 16, OPA 17, OPA 18, OPG 03, OPG 05, OPG 09, OPG 15 and 391) to amplify DNAfrom five samples of A. capillaris. A total of 335 RAPD fragments (RAPDs) with all polymorphic fragments (100%) with size ranging from 150 – 3000 bp were scored from the population. Genetic distance for samples ranges from 0.0000 to 0.2600

Efficient method for the extraction of genomic DNA from wormwood (Artemisia capillaris)

Suitable method for isolation of genomic DNA is really important. The best method can make the best result for genetic study. About five differences methods were used amongst which were Sarkosyl Method, CTAB method, Kit Method, SDS Method and Phenol-Chloroform Method. Isolated genomic DNA showed high purity and high quantity in Sarkosyl method compare the other methods. The Sarkosyl DNA isolation method is suitable for restriction digestion and genetic study for Artemisia capillaris