Adiposity and physical activity among children in countries at different stages of the physical activity transition: Canada, Mexico and Kenya

Physical inactivity is a risk factor for childhood overweight and obesity, and is associated with habitual lifestyle behaviours that are in rapid transition in many countries. Data comparing the weight status and physical activity among children in countries at different stages of the physical activity transition are lacking. The objective of this study was to compare the weight status and physical activity levels among children in Canada, Mexico and Kenya as a model to assess the physical activity transition. School children (aged 9-13 years) from Canada (n=736), Mexico (n=193) and Kenya (n=179) participated in this study. Body mass index (BMI) was used to determine weight status (underweight, normal weight, overweight and obesity). Activity monitors were used to measure daily step counts. Findings showed that 25.6% of Canadian children were overweight or obese, compared to 35.7% and 5.6% of Mexican and Kenyan children, respectively. Results indicated that 41.2% of the Kenyan children were underweight compared to 10.9% and 6.4% of Mexican and Canadian children, respectively. Mexican and Kenyan children were more physically active than Canadian children, accumulating an average of 15,757±5565, 15,605±5963 and 10,730±3969 steps per day, respectively. This inter-country comparison shows that childhood overweight and obesity levels are lowest and physical activity levels are high in Kenya, a country at an early stage of the physical activity transition. Further research using more representative samples is recommended.Keywords: Body composition, physical activity, children, transition

Identification of novel biomarker and therapeutic target candidates for acute intracerebral hemorrhage by quantitative plasma proteomics

Abstract Background The systematic mechanisms of acute intracerebral hemorrhage are still unknown and unverified, although many recent researches have indicated the secondary insults. This study was aimed to disclose the pathological mechanism and identify novel biomarker and therapeutic target candidates by plasma proteome. Methods Patients with AICH (n = 8) who demographically matched healthy controls (n = 4) were prospectively enrolled, and their plasma samples were obtained. The TMT-LC–MS/MS-based proteomics approach was used to quantify the differential proteome across plasma samples, and the results were analyzed by Ingenuity Pathway Analysis to explore canonical pathways and the relationship involved in the uploaded data. Results Compared with healthy controls, there were 31 differentially expressed proteins in the ICH group (P < 0.05), of which 21 proteins increased while 10 proteins decreased in abundance. These proteins are involved in 21 canonical pathways. One network with high confidence level was selected by the function network analysis, in which 23 proteins, P38MAPK and NFκB signaling pathways participated. Upstream regulator analysis found two regulators, IL6 and TNF, with an activation z-score. Seven biomarker candidates: APCS, FGB, LBP, MGMT, IGFBP2, LYZ, and APOA4 were found. Six candidate proteins were selected to assess the validity of the results by subsequent Western blotting analysis. Conclusion Our analysis provided several intriguing pathways involved in ICH, like LXR/RXR activation, acute phase response signaling, and production of NO and ROS in macrophages pathways. The three upstream regulators: IL-6, TNF, LPS, and seven biomarker candidates: APCS, APOA4, FGB, IGFBP2, LBP, LYZ, and MGMT were uncovered. LPS, APOA4, IGFBP2, LBP, LYZ, and MGMT are novel potential biomarkers in ICH development. The identified proteins and pathways provide new perspectives to the potential pathological mechanism and therapeutic targets underlying ICH

Accelerometer-Derived Activity Phenotypes in Young Adults: a Latent Class Analysis.

Purpose: To identify “activity phenotypes” from accelerometer-derived activity characteristics among young adults. Methods: Participants were young adults (n = 628, mean age, 22.1, SD 0.6) in the Raine Study in Western Australia. Sex-specific latent class analyses identified sub-groups using eight indicators derived from 7-day hip-worn Actigraph GT3X+ accelerometers: daily steps, total daily moderate-to-vigorous physical activity (MVPA), MVPA variation, MVPA intensity, MVPA bout duration, sedentary-to-light ratio, sedentary-to-light ratio variation, and sedentary bout duration. Results: Five activity phenotypes were identified for women (n = 324) and men (n = 304). Activity phenotype 1 for both women (35%) and men (30%) represented average activity characteristics. Phenotype 2 for women (17%) and men (16%) was characterized by below average total activity and MVPA (10.6 and 16.7 min of MVPA/day, women and men respectively). Phenotype 3 for women (15%) and men (23%) was characterized by below average total physical activity, average MVPA (32.6 and 36.5 min/day), high sedentary-light ratio and long sedentary bouts. Phenotype 4 differed between women (29%) and men (18%) but both had low sedentary-to-light ratios and shorter sedentary bouts. Finally, phenotype 5 in both women (4%) and men (12%) was characterized by extreme MVPA metrics (81.3 and 96.1 min/day). Conclusions: Five activity phenotypes were identified for each gender in this population of young adults which can help design targeted interventions to enhance or modulate activity phenotypes