Quantitative Proteomic Analysis of Human Embryonic Stem Cell Differentiation by 8-Plex iTRAQ Labelling

Analysis of gene expression to define molecular mechanisms and pathways involved in human embryonic stem cells (hESCs) proliferation and differentiations has allowed for further deciphering of the self-renewal and pluripotency characteristics of hESC. Proteins associated with hESCs were discovered through isobaric tags for relative and absolute quantification (iTRAQ). Undifferentiated hESCs and hESCs in different stages of spontaneous differentiation by embryoid body (EB) formation were analyzed. Using the iTRAQ approach, we identified 156 differentially expressed proteins involved in cell proliferation, apoptosis, transcription, translation, mRNA processing, and protein synthesis. Proteins involved in nucleic acid binding, protein synthesis, and integrin signaling were downregulated during differentiation, whereas cytoskeleton proteins were upregulated. The present findings added insight to our understanding of the mechanisms involved in hESC proliferation and differentiation

Investigating the effect of continuous care model on social health status of family caregivers in hospitalized patients undergoing coronary artery bypass graft surgery

Introduction: Chronic patient care causes &nbsp;some problems, including pressure or burden of care, reducing the time for routine activities and self care, also causes compatibility decline in deal with stress and disorders of physical and mental health, emotional, social and financial prosperity of caregivers. The aim of this study was to determine the effect of continuous care model on social health status of&nbsp; family caregivers in patients &nbsp;underwent coronary artery bypass surgeries. Methods: This study was &nbsp;one-group clinical trial research, the samples consisted 48 family caregiver of&nbsp; the patients &nbsp;underwent coronary artery bypass graft &nbsp;surgeries, who &nbsp;were selected using purposive sampling method. Data collection tools included the standard questionnaire Data collection tool was Novak and Guest caring burden inventory (CBI) s as well as demographic questionnaire, which &nbsp;was &nbsp;measured at the baseline and 8 weeks after intervention. &nbsp;Data were analyzed using&nbsp; descriptive&nbsp; and analytic statistic (paired t-test, and covariance analysis) with SPSS version 16. Results: The mean score of &nbsp;caring burden realated&nbsp; to&nbsp; the social health status before intervention was 3.86&plusmn;6.11 and after intervention was 1.81&plusmn;2.33. In examining five dimensions burden of care,&nbsp; continuous care model had an impact on all aspects of the &nbsp;caring burden and it led to the reduction of the burden of care &nbsp;in &nbsp;all aspects. &nbsp;So, &nbsp;it had a significant decrease &nbsp;in the social health status (p <0.01). Conclusion: Implementation of continuous care model as the intervention of cheaper and available, &nbsp;can be an effective step inreducing the burden of care of the &nbsp;patients with&nbsp; coronary artery disease in health status of social