26 research outputs found
Temporal covariance structure of multi-spectral phenotypes and their predictive ability for end-of-season traits in maize
Thirty loci identified for heart rate response to exercise and recovery implicate autonomic nervous system
Manufacturing and Properties of High-Velocity Oxygen Fuel (HVOF)-Sprayed FeVCrC Coatings
Identification of two p53 isoforms from Litopenaeus vannamei and their interaction with NF-κB to induce distinct immune response
Precisely and accurately localizing single emitters in fluorescence microscopy
Methods based on single-molecule localization and photophysics have brought nanoscale imaging with visible light into reach. This has enabled single-particle tracking applications for studying the dynamics of molecules and nanoparticles and contributed to the recent revolution in super-resolution localization microscopy techniques. Crucial to the optimization of such methods are the precision and accuracy with which single fluorophores and nanoparticles can be localized. We present a lucid synthesis of the developments on this localization precision and accuracy and their practical implications in order to guide the increasing number of researchers using single-particle tracking and super-resolution localization microscopy