11 research outputs found
Effect of climate on the epidemiology of bovine hypodermosis in algeria
In order to explore the effect of climate on cattle warble fly infestation, a total of 1.635 animals from 4 departments of Northern Algeria were examined visually and by manual palpation for the presence of warbles. Cattle were examined from March to
June 2014, coinciding with the peak of emergence of warbles, and both the prevalence and intensity of infestation were recorded. The departments included in this study were located in the two different climatic areas in Northern Algeria:
humid (Bejaia and Tizi Ouzou) and semi-arid (Tissemssilt and Ain Defla). The overall prevalence was 28.75%; the intensity of infestation ranged from 1 to 98 warbles per animal (mean 18.93±11.05). The prevalence and intensity of infestation within the departments with semi-arid climate (38.23%; 21.57±11.98) was significantly higher than in those with humid climate (20.74%; 14.84±7.86). The CHAID algorithm showed the climate as the most influencing factor for warble fly prevalence, followed by
the husbandry system and breed. Logistic regression and multivariate ANOVA indicate that in addition to climate, other intrinsic (age, breed) and extrinsic factors (husbandry system, treatment) included in the study also were associated with both, prevalence and intensity of infestation. Our results indicate that in semiarid areas of Northern Algeria environmental conditions are more favorable for the development of free stages (pupae and adult flies) of Hypoderma spp life-cycle than in humid areas
Pretransplant peritoneal dialysis relative to hemodialysis improves long-term survival of kidney transplant patients: a single-center observational study
Prolonged Fasting as a Method of Mood Enhancement in Chronic Pain Syndromes: A Review of Clinical Evidence and Mechanisms
Tuning morphology of mesoporous titanium oxides through fluorinated surfactants-based systems
Mycobacterium tuberculosis Uses Host Triacylglycerol to Accumulate Lipid Droplets and Acquires a Dormancy-Like Phenotype in Lipid-Loaded Macrophages
Two billion people are latently infected with Mycobacterium tuberculosis (Mtb). Mtb-infected macrophages are likely to be sequestered inside the hypoxic environments of the granuloma and differentiate into lipid-loaded macrophages that contain triacylglycerol (TAG)-filled lipid droplets which may provide a fatty acid-rich host environment for Mtb. We report here that human peripheral blood monocyte-derived macrophages and THP-1 derived macrophages incubated under hypoxia accumulate Oil Red O-staining lipid droplets containing TAG. Inside such hypoxic, lipid-loaded macrophages, nearly half the Mtb population developed phenotypic tolerance to isoniazid, lost acid-fast staining and accumulated intracellular lipid droplets. Dual-isotope labeling of macrophage TAG revealed that Mtb inside the lipid-loaded macrophages imports fatty acids derived from host TAG and incorporates them intact into Mtb TAG. The fatty acid composition of host and Mtb TAG were nearly identical suggesting that Mtb utilizes host TAG to accumulate intracellular TAG. Utilization of host TAG by Mtb for lipid droplet synthesis was confirmed when fluorescent fatty acid-labeled host TAG was utilized to accumulate fluorescent lipid droplets inside the pathogen. Deletion of the Mtb triacylglycerol synthase 1 (tgs1) gene resulted in a drastic decrease but not a complete loss in both radiolabeled and fluorescent TAG accumulation by Mtb suggesting that the TAG that accumulates within Mtb is generated mainly by the incorporation of fatty acids released from host TAG. We show direct evidence for the utilization of the fatty acids from host TAG for lipid metabolism inside Mtb. Taqman real-time PCR measurements revealed that the mycobacterial genes dosR, hspX, icl1, tgs1 and lipY were up-regulated in Mtb within hypoxic lipid loaded macrophages along with other Mtb genes known to be associated with dormancy and lipid metabolism