6 research outputs found
Andrological, pathologic, morphometric, and ultrasonographic findings in rams experimentally infected with Brucella ovis
AbstractBrucella ovis is considered the most important infectious cause of reproductive disorders in sheep. The disease is characterized by epididymitis, subfertility and infertility in rams. B. ovis occasionally results in abortion in ewes, as well. The aim of this study was to evaluate kinetic changes in the reproductive organs of rams experimentally infected with B. ovis. Nine rams were experimentally inoculated intrapreputially with 2mL of a suspension containing 1.2Ă109CFU (colony-forming units)/mL of B. ovis (strain ATCC25840). In addition, 50ÎŒL of a suspension containing 1.2Ă1010CFU/mL of the same B. ovis strain was inoculated into each conjunctival sac, resulting in 3.6Ă109CFU total per ram. Six of nine infected rams had developed clinical changes in the tail of the epididymis at 30 days post-infection (dpi), but these changes regressed in 50% of these rams. Ultrasound demonstrated an increase in the area of the tail of the epididymis (P<0.001), reduction in the area of the testes (P<0.001), and an increased length and width of the seminal vesicles (P<0.001) during the course of infection. A sperm granuloma was diagnosed on the basis of ultrasonography findings. Microscopically, there was epididymitis, testicular degeneration, and seminal vesiculitis. Inflammatory cells were detected in the semen even before the development of epididymitis. Moreover, inflammatory cells were also found in the semen of asymptomatic rams, indicating that the presence of leukocytes in the ejaculate is a valuable method for screening potential carriers of infections in the genital tract
Andrological, pathologic, morphometric, and ultrasonographic findings in rams experimentally infected with Brucella ovis
AbstractBrucella ovis is considered the most important infectious cause of reproductive disorders in sheep. The disease is characterized by epididymitis, subfertility and infertility in rams. B. ovis occasionally results in abortion in ewes, as well. The aim of this study was to evaluate kinetic changes in the reproductive organs of rams experimentally infected with B. ovis. Nine rams were experimentally inoculated intrapreputially with 2mL of a suspension containing 1.2Ă109CFU (colony-forming units)/mL of B. ovis (strain ATCC25840). In addition, 50ÎŒL of a suspension containing 1.2Ă1010CFU/mL of the same B. ovis strain was inoculated into each conjunctival sac, resulting in 3.6Ă109CFU total per ram. Six of nine infected rams had developed clinical changes in the tail of the epididymis at 30 days post-infection (dpi), but these changes regressed in 50% of these rams. Ultrasound demonstrated an increase in the area of the tail of the epididymis (P<0.001), reduction in the area of the testes (P<0.001), and an increased length and width of the seminal vesicles (P<0.001) during the course of infection. A sperm granuloma was diagnosed on the basis of ultrasonography findings. Microscopically, there was epididymitis, testicular degeneration, and seminal vesiculitis. Inflammatory cells were detected in the semen even before the development of epididymitis. Moreover, inflammatory cells were also found in the semen of asymptomatic rams, indicating that the presence of leukocytes in the ejaculate is a valuable method for screening potential carriers of infections in the genital tract
PURIFICATION AND CHARACTERIZATION OF THREEISOZYMES OF PECTIN METHYLESTERASE FROM TOMATO FRUIT
Adaptation of a Spectrophotometric Assay for Pectinmethylesterase to a Kinetic Microplate Reader
A comparison of two agar gel immunodiffusion methods and a complement fixation test for serologic diagnosis of Brucella ovis infection in experimentally infected rams
A infecção por Brucella ovis Ă© considerada uma das principais causas de epididimite e infertilidade em carneiros, resultando em falhas reprodutivas e perdas econĂŽmicas significativas em rebanhos ovinos ao redor do mundo. O estudo teve o objetivo de avaliar trĂȘs testes sorolĂłgicos disponĂveis para o diagnĂłstico da brucelose ovina por B. ovis, utilizando 181 soros ovinos. Amostras de soro provenientes de carneiros experimentalmente infectados foram coletadas ao longo de 192 dias pĂłs-infecção (n=117) e durante o perĂodo prĂ©-infecção (n=9). Adicionalmente, amostras de soro foram obtidas de ovinos provenientes de um rebanho livre para B. ovis (n=55). As tĂ©cnicas de imunodifusĂŁo em gel de agar (IDGA), utilizando dois antĂgenos disponĂveis comercialmente, e de fixação de complemento foram comparadas (FC). Foram obtidos resultados de sensibilidade especificidade semelhantes para ambos os mĂ©todos de IDGA e ainda, a tĂ©cnica de IDGA foi mais eficiente do que a da FC para o diagnĂłstico sorolĂłgico da infecção por B. ovis