Zinc adaptation and resistance to cadmium toxicity in mammalian cells. Molecular insight by proteomic analysis

To identify proteins involved in cellular adaptive responses to zinc, a comparative proteome analysis between a previously developed high zinc- and cadmium- resistant human epithelial cell line (HZR) and the parental HeLa cells has been carried out. Differentially produced proteins included co-chaperones, proteins associated with oxido-reductase activities, and ubiquitin. Biochemical pathways to which these proteins belong were probed for their involvement in the resistance of both cell lines against cadmium toxicity. Among endoplasmic reticulum stressors, thapsigargin sensitized HZR cells, but not HeLa cells, to cadmium toxicity more acutely than tunicamycin, implying that these cells heavily relied on proper intracellular calcium distribution. The similar sensitivity of both HeLa and HZR cells to inhibitors of the proteasome, such as MG-132 or lactacystin, excluded improved proteasome activity as a mechanism associated with zinc adaptation of HZR cells. The enzyme 4-hydroxyphenylpyruvate dioxygenase was overproduced in HZR cells as compared to HeLa cells. It transforms 4-hydroxyphenylpyruvate to homogentisate in the second step of tyrosine catabolism. Inhibition of 4-hydroxyphenylpyruvate dioxygenase decreased the resistance of HZR cells against cadmium, but not that of HeLa cells, suggesting that adaptation to zinc overload and increased 4-hydroxyphenylpyruvate removal are linked in HZR cellsComment: in press in Proteomic

Misdiagnosed Tooth Aspiration in a Young Handicapped Boy: Case Report and Recommendations

Tooth inhalation remains a rare incident but it may occur during dental care, especially in children. We report here the case of a four-year-old boy with Down syndrome who came to the hospital after a dental trauma. During the extraction procedure, he aspired his maxillary incisor without presenting any signs of respiratory distress and was discharged by the surgical team, who thought that he had swallowed the tooth. Three weeks later, he was admitted to the emergency service because of a pulmonary infection. Two endoscopy interventions under general anesthesia were necessary to recover the foreign body inside the left lung. Because of the multiple symptoms associated with the trisomy 21 syndrome (general hypotonia, impaired immunity, etc.), practitioners should be very mindful of aspiration risks and complications during dental care. The systematic prescription of lung radiography would prevent the onset of pulmonary infections and enable an earlier intervention

La maltraitance des enfants, rôle de l'odontologiste

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Odontologie (341722110) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Optimisation de l'anesthésie locale chez l'enfant

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Odontologie (341722110) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Intérêt du localisateur d'apex en denture temporaire (étude clinique sur un échantillon de 58 canaux)

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Odontologie (341722110) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

La gestion des traumatismes en denture permanente immature

Les traumatismes dentaires peuvent survenir à n'importe quel âge, nous nous intéresserons ici aux dents immatures traumatisées. Après un rappel des constituants de la dent et du parodonte, nous nous intéresserons à l'épidémiologie des traumatismes, la prise en charge de l'enfant traumatisé, au diagnostic des traumatismes et à leur traitement pour enfin voir les complications liées aux lésions traumatiques. Pour finir, dans une troisième et dernière partie nous évoquerons le principes de prévention au quotidien et lors des activités sportives.Dental traumas can happen in any age, we shaH be interested in the traumatized childish teeth here. After a recall of (constituents the tooth and of the parodonte, we shaH be interested in the epidemiology of traumas, the taking care of the traumatized child, in the diagnosis of traumas and in their treatment to see finally complications linked to traumatic lesions. To finish, in a third and last party we shaH recall the principles of prevention in the daily and during sports activities.MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Odontologie (341722110) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Les reconstitutions prothétiques en odontologie pédiatrique

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Odontologie (341722110) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

Prise en charge de l'anxiété et de la douleur par l'hypnose lors des soins dentaires,ou comment améliorer la communication avec les patients

L 'hypnose est un outil encore très peu utilisé en Odontologie. Cette thèse en fait une approche dans la pratique quotidienne d'un cabinet dentaire. Dans un premier temps, nous verrons pourquoi la relation patient-praticien est si particulière et source d'anxiété, même de nos jours. Ensuite un rappel de l'histoire de l'hypnose permettre de connaitre ses origines et ainsi de mieux comprendre ses utilisations dans la pratique actuelle. Enfin nous proposons quelques adresses utiles pour les personnes souhaitant se former, ou voir des applications sous forme de vidéos.The hypnosis is a too1 still1itt1e used in Odontology. This thesis present an approach in the dai1y practice of a dentist's surgery.At first, we want to see why the relation patient-practitioner is so particular and source of anxiety, even nowadays. Them a reminder of the history of the hypnosis permite to know her origins and so to understand better its uses in the current practice. Finally we propose sorne useful addresses for the persons wishing to form, or to see applications in the form of videos.MONTPELLIER-BU Odontologie (341722110) / SudocMONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

La sédation consciente par inhalation de protoxyde d'azote en odontologie pédiatrique

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Odontologie (341722110) / SudocPARIS-BIUM (751062103) / SudocMONTPELLIER-BU Médecine (341722104) / SudocSudocFranceF

L'anesthésie générale en odontologie pédiatrique

MONTPELLIER-BU Médecine UPM (341722108) / SudocMONTPELLIER-BU Odontologie (341722110) / SudocMONTPELLIER-BU Médecine (341722104) / SudocPARIS-BIUM (751062103) / SudocSudocFranceF