38 research outputs found
Calcium regulates acid-sensing ion channel 3 activation by competing with protons in the channel pore and at an allosteric binding site
The extracellular Ca2+ concentration changes locally under certain physiological and pathological conditions. Such variations affect the function of ion channels of the nervous system, and consequently also neuronal signalling. We investigated here the mechanisms by which Ca2+ controls the activity of acid-sensing ion channel (ASIC) 3. ASICs are neuronal, H+-gated Na+ channels involved in several physiological and pathological processes, including the expression of fear, learning, pain sensation and neurodegeneration after ischemic stroke. It was previously shown that Ca2+ negatively modulates the ASIC pH dependence. While protons are default activators of ASIC3, this channel can also be activated at pH7.4 by removal of the extracellular Ca2+. Two previous studies concluded that low pH opens ASIC3 by displacing Ca2+ ions that block the channel pore at physiological pH. We show here that an acidic residue, distant from the pore, controls, together with pore residues, the modulation of ASIC3 by Ca2+. Our study identifies a new regulatory site in ASIC3 and demonstrates that ASIC3 activation involves an allosteric mechanism together with Ca2+ unbinding from the channel pore. We provide a molecular analysis of a regulatory mechanism found in many ion channels
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Effect of interpersonal dysfunction on treatment response to a cognitive-behavioral stress management intervention in men treated for early stage prostate cancer
The present study sought to evaluate the efficacy of a 10-week, group based intervention in promoting sexual function and in decreasing biological markers of distress in older men recovering from localized prostate cancer. Additionally, investigators attempted to determine the effect of certain underlying personality traits (i.e., interpersonal problems) on the efficacy of this intervention. Participants were 215 middle-aged and older men (M age = 64.8, SD = 7.6) recovering from localized (i.e., Stage I-II) prostatic carcinoma, and had undergone either radical prostatectomy or external beam radiotherapy. Participants were randomized into either a 10-week, group based Cognitive-Behavioral Stress Management (CBSM) intervention (experimental condition) or a one-day educational seminar (control condition). Self-report and biological measures were completed prior to randomization and again following CBSM or control interventions. Dependent measures included the Functional Assessment of Cancer Therapies, the Psychosocial Adjustment to Illness Scale and urinary catecholamine production. Interpersonal dysfunction was measured using the Inventory of Interpersonal Problems. Hypotheses were evaluated using both structural equation modeling and moderated regression. Results suggested that the CBSM intervention was effective in promoting sexual satisfaction and sexual functioning in men treated with radical prostatectomy, but not in men treated with radiotherapy. Further, the effect on sexual functioning in men treated with radical prostatectomy was highest for participants reporting greater levels of interpersonal dysfunction at baseline. There was no effect on urinary catecholamine production. The fact that the CBSM intervention was effective primarily for men recovering from radical prostatectomy is discussed in terms of its timing, i.e. the placement of the intervention within the natural trajectory of recovery following surgical treatment. Results also suggest that individuals with higher levels of interpersonal dysfunction may make the greatest gains from group-based interventions, presumably related to lower baseline mood and quality of life and greater room to grow
PRZEDRUK POLSKIEGO T艁UMACZENIA ARTYKU艁U ZA ZGOD膭 INTERNATIONAL ASSOCIATION FOR THE STUDY OF PAIN (IASP) - PAIN 154 (2013) 2775-2781 Warto艣膰 rokownicza strategii radzenia sobie z b贸lem w wybranej z populacji pr贸bie os贸b z przewlek艂ymi objawowymi zmianami zwyrodnieniowymi staw贸w kolanowych
Radiographic knee osteoarthritis (OA) is a highly prevalent condition that has been the focus of a number of studies identifying factors that are prognostic of continued or worsening pain and function. Although prior prognostic studies have identified a number of demographic, physical, and psychological factors that are predictive of outcome, minimal focus has been placed on pain coping skills as prognostic factors, despite crosssectional evidence suggesting that pain coping skills are associated with pain and function in knee OA. The present study reports on the use of pain coping skills as prognostic factors for changes in pain and/or function over a 1-year period. Participants were drawn from the Osteoarthritis Initiative, a prospective longitudinal cohort study of persons recruited from the community who either had knee OA or were at high risk for developing knee OA. Data from the Coping Strategies Questionnaire were compared against 1-year change in pain, function, or both, using established criteria for defining whether the patient got better, worse, or stayed the same over the 1-year period. Results revealed a significant effect for praying/hoping, increased behavioral activities, and pain catastrophizing as prognostic of pain outcomes; ignoring pain and praying/hoping were prognostic of function outcomes; and increased behavioral activities and pain catastrophizing were prognostic of a combined pain and function outcome. The findings provide important new evidence regarding the potential clinical relevance of a number of pain coping responses hypothesized to influence future pain and function in persons with arthritis.Stwierdzane radiograficznie zmiany zwyrodnieniowe staw贸w kolanowych to schorzenie bardzo cz臋ste i b臋d膮ce przedmiotem licznych bada艅, w kt贸rych identyfikowano czynniki rokownicze utrzymuj膮cego si臋 lub narastaj膮cego b贸lu i zaburze艅 czynno艣ci. We wcze艣niejszych badaniach dotycz膮cych rokowania zidentyfikowano liczne czynniki demograficzne, fizyczne i psychologiczne, kt贸re s膮 predyktorami wyniku leczenia, ale niewiele uwagi po艣wi臋cono umiej臋tno艣ciom radzenia sobie z b贸lem jako czynnikowi rokowniczemu, pomimo danych pochodz膮cych z bada艅 przekrojowych, kt贸re wskazuj膮, 偶e umiej臋tno艣ci te wi膮偶膮 si臋 z b贸lem i z funkcjonowaniem w chorobie zwyrodnieniowej staw贸w kolanowych. W bie偶膮cym badaniu opisujemy zastosowanie umiej臋tno艣ci radzenia sobie z b贸lem jako jednym z czynnik贸w rokowniczych zmian w zakresie b贸lu i/lub funkcjonowania w ci膮gu rocznej obserwacji. Uczestnicy badania pochodzili z Osteorthritis Initiative, prospektywnego pod艂u偶nego badania kohortowego os贸b rekrutowanych ze spo艂eczno艣ci, kt贸re mia艂y chorob臋 zwyrodnieniow膮 staw贸w kolanowych lub by艂y obci膮偶one du偶ym ryzykiem rozwoju tego schorzenia. Dane z Coping Strategies Questionnaire por贸wnano z rocznymi zmianami w zakresie b贸lu, czynno艣ci lub obu tych zmiennych, za pomoc膮 okre艣lonych kryteri贸w definiuj膮cych zmian臋 stanu klinicznego pacjenta na lepszy, gorszy lub bez zmian w ci膮gu roku. Wyniki wskaza艂y na istotne efekty modlitwy/nadziei, zwi臋kszonej aktywno艣ci behawioralnej i my艣lenia katastroficznego na temat b贸lu, jako czynnik贸w rokowniczych wyniku leczenia; ignorowanie b贸lu oraz modlitwa/nadzieja by艂y czynnikami prognostycznymi wyniku leczenia w zakresie funkcjonowania; zwi臋kszona aktywno艣膰 behawioralna i my艣lenie katastroficzne na temat b贸lu by艂y czynnikami rokowniczymi odnosz膮cymi si臋 艂膮cznie do wynik贸w leczenia b贸lu oraz funkcjonowania. Wyniki dostarczaj膮 istotnych nowych danych naukowych odno艣nie do potencjalnego klinicznego znaczenia wielu reakcji radzenia sobie z b贸lem, kt贸re przypuszczalnie mog膮 wp艂ywa膰 na przysz艂y b贸l i funkcjonowanie os贸b z chorob膮 zwyrodnieniow膮 staw贸w
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Physical activity and sexual functioning after radiotherapy for prostate cancer: Beneficial effects for patients undergoing external beam radiotherapy
To evaluate, in a cross-sectional study, the relationships among physical activity, sexual functioning, and treatment type for 111 men who had undergone radiotherapy for localized prostate cancer within the past 18 months. Physical activity preserves the sexual functioning capacity of older men. However, little information exists regarding the association of physical activity with sexual functioning after treatment for localized prostate cancer.
We tested the main effects of physical activity and treatment procedure, as well as their interaction, using hierarchical regression analysis. We hypothesized that greater physical activity would relate to better reported sexual functioning and that this relationship would be moderated by the type of medical treatment.
After controlling for age, medical comorbidity, fatigue, and urinary and bowel functioning, more physical activity was significantly associated with better sexual functioning, and the interaction of physical activity and treatment procedure added a significant amount of explained variance. Overall, 35% of the variance in sexual functioning was accounted for by the model. Post hoc tests of moderation revealed that men who underwent external beam radiotherapy had significantly greater sexual functioning scores as physical activity increased but the effect of physical activity on sexual function after brachytherapy and combination therapy was nonsignificant.
Physical activity was positively correlated with sexual functioning for those who underwent external beam radiotherapy. These relationships should be replicated and explored in a larger, longitudinal sample to ascertain whether the effects of physical activity in this at-risk population extend over time and protect men from treatment-related decrements in sexual functioning
Calcium regulates acid-sensing ion channel 3 activation by competing with protons in the channel pore and at an allosteric binding site
The extracellular Ca; 2+; concentration changes locally under certain physiological and pathological conditions. Such variations affect the function of ion channels of the nervous system and consequently also neuronal signalling. We investigated here the mechanisms by which Ca; 2+; controls the activity of acid-sensing ion channel (ASIC) 3. ASICs are neuronal, H; +; -gated Na; +; channels involved in several physiological and pathological processes, including the expression of fear, learning, pain sensation and neurodegeneration after ischaemic stroke. It was previously shown that Ca; 2+; negatively modulates the ASIC pH dependence. While protons are default activators of ASIC3, this channel can also be activated at pH7.4 by the removal of the extracellular Ca; 2+; . Two previous studies concluded that low pH opens ASIC3 by displacing Ca; 2+; ions that block the channel pore at physiological pH. We show here that an acidic residue, distant from the pore, together with pore residues, controls the modulation of ASIC3 by Ca; 2+; . Our study identifies a new regulatory site in ASIC3 and demonstrates that ASIC3 activation involves an allosteric mechanism together with Ca; 2+; unbinding from the channel pore. We provide a molecular analysis of a regulatory mechanism found in many ion channels
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Cognitive behavioral stress management intervention improves quality of life in Spanish monolingual hispanic men treated for localized prostate cancer: results of a randomized controlled trial
The efficacy of a group-based psychosocial intervention with ethnic minority health populations may depend on consideration for cultural factors that can interact with group processes.
The current study explored the efficacy of a 10-week group-based cognitive-behavioral stress management (CBSM) intervention that was linguistically and culturally adapted for use with Hispanic monolingual men recently treated for localized prostate carcinoma (PC).
71 Hispanic mono-lingual Spanish speakers were randomly assigned to a 10-week CBSM intervention or a half-day stress management seminar (control condition). Hierarchical regression was used to predict post-intervention QoL.
After controlling for relevant covariates, assignment to the CBSM condition significantly predicted greater physical well-being, emotional well-being, sexual functioning, and total well-being after the 10-week intervention period.
Results suggest that participation in a culturally and linguistically adapted CBSM group intervention improved QoL in Hispanic monolingual men treated for localized PC
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Cognitive Behavioral Stress Management Intervention Improves Quality of Life in Spanish Monolingual Hispanic Men Treated for Localized Prostate Cancer: Results of a Randomized Controlled Trial
The efficacy of a group-based psychosocial intervention with ethnic minority health populations may depend on consideration for cultural factors that can interact with group processes. The current study explored the efficacy of a 10-week group-based cognitive-behavioral stress management (CBSM) intervention that was linguistically and culturally adapted for use with Hispanic monolingual men recently treated for localized prostate carcinoma (PC). 71 Hispanic mono-lingual Spanish speakers were randomly assigned to a 10-week CBSM intervention or a half-day stress management seminar (control condition). Hierarchical regression was used to predict post-intervention QoL. After controlling for relevant covariates, assignment to the CBSM condition significantly predicted greater physical well-being, emotional well-being, sexual functioning, and total well-being after the 10-week intervention period. Results suggest that participation in a culturally and linguistically adapted CBSM group intervention improved QoL in Hispanic monolingual men treated for localized PC