Feasibility of Using Short Message Service (SMS) to Collect Outcome Data in an Australian Residential Alcohol and Drug Treatment Service

Objectives: Limited studies have examined the utility of short message service (SMS) for outcome data collection, and none have examined its feasibility within a realistic alcohol and other drug (AoD) treatment environment. The objective of this study was to investigate SMS outcome data collection with limited resources within an Australian residential AoD treatment setting. Methods: A total of 153 clients engaging in residential AoD treatment completed the study; 137 completed feasibility surveys and 19 residents completed a 16-week SMS trial utilizing an automated web-based application, which sent 4 single-items by SMS each week. Feasibility was evaluated by willingness ratings, reasons for nonengagement, proportion of consenting participants, response rates, cost and ease of implementation. The validity of single-item measures was assessed using correlational analyses. Results: Totally, 64.6% of participants were unwilling to engage in SMS monitoring, with the main reasons for not engaging being cost and disinterest in research. Twelve percent of participants engaged in the SMS trial, with 90.5% providing at least 1 SMS response. Although initial responses took up to 6 hours on average, subsequent items were completed within 1 to 5 minutes. Conclusions: In its present form, this method of data collection was not feasible within these AoD residential services. Greater resources are likely required to improve the utility and acceptability of this method

Structures of lipoprotein signal peptidase II from Staphylococcus aureus complexed with antibiotics globomycin and myxovirescin.

Antimicrobial resistance is a major global threat that calls for new antibiotics. Globomycin and myxovirescin are two natural antibiotics that target the lipoprotein-processing enzyme, LspA, thereby compromising the integrity of the bacterial cell envelope. As part of a project aimed at understanding their mechanism of action and for drug development, we provide high-resolution crystal structures of the enzyme from the human pathogen methicillin-resistant Staphylococcus aureus (MRSA) complexed with globomycin and with myxovirescin. Our results reveal an instance of convergent evolution. The two antibiotics possess different molecular structures. Yet, they appear to inhibit identically as non-cleavable tetrahedral intermediate analogs. Remarkably, the two antibiotics superpose along nineteen contiguous atoms that interact similarly with LspA. This 19-atom motif recapitulates a part of the substrate lipoprotein in its proposed binding mode. Incorporating this motif into a scaffold with suitable pharmacokinetic properties should enable the development of effective antibiotics with built-in resistance hardiness