12 research outputs found
The λ Red Proteins Promote Efficient Recombination between Diverged Sequences: Implications for Bacteriophage Genome Mosaicism
Genome mosaicism in temperate bacterial viruses (bacteriophages) is so great that it obscures their phylogeny at the genome level. However, the precise molecular processes underlying this mosaicism are unknown. Illegitimate recombination has been proposed, but homeologous recombination could also be at play. To test this, we have measured the efficiency of homeologous recombination between diverged oxa gene pairs inserted into λ. High yields of recombinants between 22% diverged genes have been obtained when the virus Red Gam pathway was active, and 100 fold less when the host Escherichia coli RecABCD pathway was active. The recombination editing proteins, MutS and UvrD, showed only marginal effects on λ recombination. Thus, escape from host editing contributes to the high proficiency of virus recombination. Moreover, our bioinformatics study suggests that homeologous recombination between similar lambdoid viruses has created part of their mosaicism. We therefore propose that the remarkable propensity of the λ-encoded Red and Gam proteins to recombine diverged DNA is effectively contributing to mosaicism, and more generally, that a correlation may exist between virus genome mosaicism and the presence of Red/Gam-like systems
Comparison between Coffea arabica L. ‘Laurina’ and C. arabica ‘Bourbon’ seedlings grown in daylight or darkness for their polysaccharidic cell wall composition and caffeine and chlorogenic acid contents
The role of ‘accompagnement’ in the end-of-life debate in France: from solidarity to autonomy
Orcein, C- and NOR-banding on mitotic chromosomes of some wild diploid coffee species ( Coffea
Variations in cell wall monosaccharide composition during seed development in Coffea arabica L. Comparison between Coffea arabica var. Bourbon and Coffea arabica var. Laurina
Common urban birds continue to perceive predator calls that are overlapped by road noise
The B. subtilis Accessory Helicase PcrA Facilitates DNA Replication through Transcription Units
Impact of environmental factors on the population dynamics, density and foraging activities of Odontotermes lokanandi and Microtermes obesi in Islamabad
Replication–transcription conflicts in bacteria
DNA replication and transcription use the same template and occur concurrently in bacteria. The lack of temporal and spatial separation of these two processes leads to their conflict, and failure to deal with this conflict can result in genome alterations and reduced fitness. In recent years major advances have been made in understanding how cells avoid conflicts between replication and transcription and how such conflicts are resolved when they do occur. In this Review, we summarize these findings, which shed light on the significance of the problem and on how bacterial cells deal with unwanted encounters between the replication and transcription machineries.National Institutes of Health (U.S.) (grant GM084003)National Institutes of Health (U.S.) (grant GM41934)National Institutes of Health (U.S.) (postdoctoral fellowship GM093408)University of Washington. Department of MicrobiologyCancer Prevention and Research Institute of Texas (Training Program grant RP101499