7 research outputs found
Disposable array sensor strip for quantification of sinensetin in orthosiphon stamineus benth samples
A disposable screen printed array sensor
strip based on self-plasticized lipid membranes combined
with chemometric algorithm has been developed
and applied for quantification of Orthosiphon stamineus
Benth extracts. Sinensetin, a pharmacologically
active flavonoid in Orthosiphon stamineus Benth,
was quantified with the sensor system using standard
addition method. The method was compared with high
performance thin layer chromatography (HPTLC).
Partial least square (PLS) and principal component
regression (PCR) were applied to the array sensor
output to determine the sinensetin in O. stamineus
samples from different suppliers. Comparison between
the PLS and PCR models presented in the quantitative
analysis showed that PLS have substantially better
predictive capability than PCR. The root mean square
error (RMSE) of Prediction for PLS and PCR were
0.17 ppm and 0.19 ppm, respectively. The concentration
of sinensetin by PLS fell within the range of
0.25%–0.30% in six different batches of extracts that
were supplied by Hovid Sdn Bhd (HV) while a range
0.18%–0.24% was obtained in ten different batches of
extracts supplied by Nusantara Herbs Sdn Bhd (NH).
The array sensor showed good correlation (0.9902)
with the HPTLC method