11 research outputs found
First Report of an Extensively Drug-Resistant VIM-2 Metallo-ÎČ-Lactamase-Producing Brevundimonas diminuta Clinical Isolate
Complete genome sequencing of Comamonas kerstersii 8943, a causative agent for peritonitis
Chronic lower extremity wound infection due to Kerstersia gyiorum in a patient with Buergerâs disease: a case report
First report of Wohlfahrtiimonas chitiniclastica from soft tissue and bone infection at an unusually high northern latitude
Wohlfahrtiimonas larvae sp. nov., isolated from the larval gut of Hermetia illucens (Diptera: Stratiomyidae)
An Overview of the Treatment of Less Common NonâLactoseâFermenting GramâNegative Bacteria
Composition and pathogenic potential of a microbial bioremediation product used for crude oil degradation
Aggregative adherent strains of Corynebacterium pseudodiphtheriticum enter and survive within HEp-2 epithelial cells
Corynebacterium pseudodiphtheriticum is a well-known human pathogen that mainly causes respiratory disease and is associated with high mortality in compromised hosts. Little is known about the virulence factors and pathogenesis of C. pseudodiphtheriticum. In this study, cultured human epithelial (HEp-2) cells were used to analyse the adherence pattern, internalisation and intracellular survival of the ATCC 10700 type strain and two additional clinical isolates. These microorganisms exhibited an aggregative adherence-like pattern to HEp-2 cells characterised by clumps of bacteria with a "stacked-brick" appearance. The differences in the ability of these microorganisms to invade and survive within HEp-2 cells and replicate in the extracellular environment up to 24 h post infection were evaluated. The fluorescent actin staining test demonstrated that actin polymerisation is involved in the internalisation of the C. pseudodiphtheriticum strains. The depolymerisation of microfilaments by cytochalasin E significantly reduced the internalisation of C. pseudodiphtheriticum by HEp-2 cells. Bacterial internalisation and cytoskeletal rearrangement seemed to be partially triggered by the activation of tyrosine kinase activity. Although C. pseudodiphtheriticum strains did not demonstrate an ability to replicate intracellularly, HEp-2 cells were unable to fully clear the pathogen within 24 h. These characteristics may explain how some C. pseudodiphtheriticum strains cause severe infection in human patients