Human histone demethylase KDM6B can catalyse sequential oxidations

Jumonji domain‐containing demethylases (JmjC‐KDMs) catalyse demethylation of Nε ‐methylated lysines on histones and play important rolesin gene regulation. We report selectivity studies on KDM6B (JMJD3), a disease‐relevant JmjC‐KDM, using synthetic lysine analogues. The results unexpectedly reveal that KDM6B accepts multiple Nε ‐alkylated lysine analogues, forming alcohol, aldehyde and carboxylic acid products