5 research outputs found
Digestibility of resistant starch containing preparations using two in vitro models
BACKGROUND: Resistant starch (RS) is known for potential health benefits in the human colon. To investigate these positive effects it is important to be able to predict the amount, and the structure of starch reaching the large intestine. AIM OF THE STUDY: The aim of this study was to compare two different in vitro models simulating the digestibility of two RS containing preparations. METHODS: The substrates, high amylose maize (HAM) containing RS type 2, and retrograded long chain tapioca maltodextrins (RTmd) containing RS type 3 were in vitro digested using a batch and a dynamic model, respectively. Both preparations were characterized before and after digestion by using X-Ray and DSC, and by measuring their total starch, RS and protein contents. RESULTS: Using both digestion models, 60-61 g/100 g of RTmd turned out to be indigestible, which is very well in accordance with 59 g/100 g found in vivo after feeding RTmd to ileostomy patients. In contrast, dynamic and batch in vitro digestion experiments using HAM as a substrate led to 58 g/100 g and 66 g/100 g RS recovery. The degradability of HAM is more affected by differences in experimental parameters compared to RTmd. The main variations between the two in vitro digestion methods are the enzyme preparations used, incubation times and mechanical stress exerted on the substrate. However, for both preparations dynamically digested fractions led to lower amounts of analytically RS and a lower crystallinity. CONCLUSIONS: The two in vitro digestion methods used attacked the starch molecules differently, which influenced starch digestibility of HAM but not of RTmd
Comparison of color, anti-nutritional factors, minerals, phenolic profile and protein digestibility between hard-to-cook and easy-to-cook grains from different kidney bean (Phaseolus vulgaris) accessions
The potato and its contribution to the human diet and health.
Potato has contributed to human diet for thousands of years, first in the Andes of South America and then in the rest of the world. Its contribution to the human diet is affected by cooking, potato intake levels, and the bioavailability of potato nutrients. Generally, the key nutrients found in potatoes including minerals, proteins, and dietary fiber are well retained after cooking. Vitamins C and B6 are significantly reduced after cooking while carotenoids and anthocyanins show high recoveries after cooking due to an improved release of these antioxidants. In many developed countries potatoes are consumed as a vegetable with intakes that vary from 50 to 150 g per day for adults. On the other hand, in some rural areas of Africa and in the highlands of Latin American countries, potato is considered a staple crop and is consumed in large quantities with intakes that vary from 300 to 800 g per day for adults. These marked differences in the potato intake affect significantly the contribution of potato nutrients to the human dietary requirements. In recent years, information about nutrient bioaccessibility and bioavailability from potatoes has become available indicating higher bioaccessibility of minerals and vitamins in potato as compared with other staple crops such as beans or wheat. Bioavailability refers to the fraction of an ingested nutrient that is available for utilization in normal physiological functions and/or for body storage while bioaccessibility refers to the amount that is potentially absorbable from the gut lumen. In addition, potatoes have shown promising health-promoting properties in human cell culture, experimental animal and human clinical studies, including anticancer, hypocholesterolemic, anti-inflammatory, anti-obesity, and antidiabetic properties with phenolics, anthocyanins, fiber, resistant starch, carotenoids as well as glycoalkaloids contributing to the health benefits of potatoes