Changes in antioxidant capacity and colour associated with the formation of beta-carotene epoxides and oxidative cleavage derivatives

Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)In this study HPLC-DAD-MS/MS was applied for the identification of compounds derived from (all-E)-beta-carotene following epoxidation and oxidative cleavage. The consequences on the CIELAB colour parameters and antioxidant capacity (AC) were also evaluated. Five apocarotenoids, three secocarotenoids, seven Z isomers and two epoxides were detected as a result of the oxidative cleavage. Four epoxides and three Z isomers were detected as a consequence of the epoxidation reaction. Some compounds were detected for the first time as a result of oxidation reactions. Both treatments led to a marked decrease in b* and C-ab* values, indicating that these colour parameters can be used for the rapid assessment of beta-carotene oxidation. The oxidative cleavage of beta-carotene resulted in increased capacity to both scavenge ABTS(+) and quench singlet oxygen. These results suggest that the study of the AC of these oxidative derivatives and their possible usefulness as food ingredients deserves further attention. (C) 2013 Elsevier Ltd. All rights reserved.147160169Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)European Union [FP7-224789]Spanish program Ramon y. Cajal [RYC-2010-07115]Spanish program Ramon Juan de la Cierva [JCI-2008-1767]CYTED [112RT0445]Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)European Union [FP7-224789]Spanish program Ramon y. Cajal [RYC-2010-07115]Spanish program Ramon Juan de la Cierva [JCI-2008-1767]CYTED [112RT0445