Functional Analysis and Molecular Dynamics Simulation of LOX-1 K167N Polymorphism Reveal Alteration of Receptor Activity

The human lectin-like oxidized low density lipoprotein receptor 1 LOX-1, encoded by the ORL1 gene, is the major scavenger receptor for oxidized low density lipoprotein in endothelial cells. Here we report on the functional effects of a coding SNP, c.501G>C, which produces a single amino acid change (K>N at codon 167). Our study was aimed at elucidating whether the c.501G>C polymorphism changes the binding affinity of LOX-1 receptor altering its function. The presence of p.K167N mutation reduces ox-LDL binding and uptake. Ox-LDL activated extracellular signal-regulated kinases 1 and 2 (ERK 1/2) is inhibited. Furthermore, ox-LDL induced biosynthesis of LOX-1 receptors is dependent on the p.K167N variation. In human macrophages, derived from c.501G>C heterozygous individuals, the ox-LDL induced LOX-1 46 kDa band is markedly lower than in induced macrophages derived from c.501G>C controls. Investigation of p.K167N mutation through molecular dynamics simulation and electrostatic analysis suggests that the ox-LDL binding may be attributed to the coupling between the electrostatic potential distribution and the asymmetric flexibility of the basic spine residues. The N/N-LOX-1 mutant has either interrupted electrostatic potential and asymmetric fluctuations of the basic spine arginines

The contribution of molecular data to our understanding of cephalopod evolution and systematics: a review

Figure 2. Polyploidy scenario suggested by and reproduced from Bonnaud et al. (2004). Updated diploid chromosome numbers (see Table 2) for the various branches are now Nautiloidea 52; Octopoda 56–60; Sepiolida 74; Sepiida 48–112; Myopsida 86–92 (?22-?172).Published as part of Allcock, A. Louise, Lindgren, A. & Strugnell, J.M., 2014, The contribution of molecular data to our understanding of cephalopod evolution and systematics: a review, pp. 1373-1421 in Journal of Natural History 49 (21) on page 1392, DOI: 10.1080/00222933.2013.825342, http://zenodo.org/record/400623

The Role of Ego-Resiliency as Mediator of the Longitudinal Relationship between Family Socio-Economic Status and School Grades

Family socio-economic status (SES) represents one of the major determinants of youth’s scholastic achievement, and thus it is important to unravel the psychological factors underlining this relation. In this article, we examined youth’s ability to flexibly adapt and, thus, cope with harsh environmental conditions—assessed by the construct of ego-resiliency—as a mediating mechanism in the across-time association between family SES and academic achievement. The longitudinal sample was composed of 265 (56% females) Italian students who were about 13 years old at Time 1 (T1) and about 18 years old at Time 2 (T2). In a structural equation model analysis, family SES significantly predicted ego-resiliency 6 years later while controlling for the latter’s strong longitudinal stability. Students’ school grades at the end of senior high school were also predicted by ego-resiliency assessed at the age of 13, controlling for grades in the last year of junior high school, gender, and initial differences in ages. In accordance with the posited hypothesis, this study provided support for a two-wave meditational model in which the relation between family SES at 13 years and later school grades at 19 years was mediated by ego-resiliency. All in all, results support the argument that being resilient, and thus being able to flexibly adapt one’s own emotional state and resultant behavior, matters to school success