IL-1β, IL-6 and TNF-α quantitization in the serum of six groups.

<p>A. ELISA confirmation of TNF-α in the serum of six groups. Each bar represents the mean ± S.D, significant differences were found between L group and L+P Group(*p<0.01, n = 6). B. ELISA confirmation of IL-1β in the serum of four groups. Each bar represents the mean ± S.D, significant differences were found between L group and L+P Group(*p<0.01, n = 6). C. ELISA confirmation of IL-6 in the serum of four groups. Each bar represents the mean ± S.D, significant differences were found between L group and L+P Group (*p<0.01, n = 6).</p

IL-1β, IL-6 and TNF-α quantitization in the serum of THP-1 cells.

<p>ELISA confirmation of TNF-α IL-1β, IL-6 in the serum of THP-1 cells. Each bar represents the mean ± S.D, significant differences were found between L group and L+P Group(*p<0.001, compared with C and P groups; **p<0.01, compared with the L groups).</p

List of Identified Protein Spots Differentially Expressed in control, LPS, and LPS plus propofol groups.

<p>List of Identified Protein Spots Differentially Expressed in control, LPS, and LPS plus propofol groups.</p

Primers pairs used in this study.

<p>Primers pairs used in this study.</p

The impact of propofol on Annexin A1 and phosphorylation of p38 following LPS stimulation was validated and the time and concentration effects were analyzed with Western Blot.

<p>A. Lysis samples of THP-1 cells from 5 µg/ml to 50 µg/ml of propofol treated were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of Annexin A1. B. Gray intensity analysis of the western blot results of seven groups. Each bar represents the mean ± S.D, (*p<0.01, n = 3, compared with C and L groups in 6 h) C group: control group; L group: LPS group; L+P(5, 10, 20, 30, 50) group: LPS+different concentration (5, 10, 20, 30, 50 µg/ml)propofol group. C. Lysis samples of THP-1 cells from 1 h to 6 h were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of Annexin A1. D. Gray intensity analysis of the western blot results of four groups. Each bar represents the mean ± S.D, (*p<0.01, n = 3, compared with C and L groups in 6 h) C group: control group; L group: LPS group; P group: propofol group; L+P group: LPS+propofol group. E. Lysis samples of THP-1 cells from 1 h to 6 h were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of phos-p38. F. Gray intensity analysis of the western blot results of four groups. Each bar represents the mean ± S.D, (**p<0.001, compared with the C and P groups in 0.5 h, 1 h, 2 h, 6 h; **p<0.05, compared with the L groups in 2 h and 6 h, n = 3) C group: control group; L group: LPS group; P group: propofol group; L+P group: LPS+propofol group. G. Lysis samples of THP-1 cells were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of Annexin A1. H. Gray intensity analysis of the western blot results of six groups. Each bar represents the mean ± S.D. No significant differences were found between L group and L+I Group (p>0.05). C group: control group; L group: LPS group; I group: intralipid group; L+I group: LPS+intralipid group. I. Lysis samples of THP-1 cells were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of phos-p38. J. Gray intensity analysis of the western blot results of four groups. Each bar represents the mean ± S.D. No significant differences were found between L group and L+I Group (p>0.05). C group: control group; L group: LPS group; I group: intralipid group; L+I group: LPS+intralipid group.</p

Confirming the expression of Annexin A1 and phos-p38 in monocytes with Western Blot assay.

<p>A. Monocytes lysis samples from the individual rats were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis. B. Gray intensity analysis of the western blot results of six groups. Each bar represents the mean ± S.D, (*p<0.01, n = 6, compared with the other four groups) C group: control group; L group: LPS group; P group: propofol group; L+P group: LPS+propofol group; I group: intralipid group; L+I group: LPS+intralipid group. C. Monocytes lysis samples from the individual rats were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis. D. Gray intensity analysis of the western blot results of four groups. Each bar represents the mean ± S.D, (**p<0.01, n = 6, compared with the C, P and I groups; ***p<0.05, n = 6, compared with the L and L+I groups) C group: control group; L group: LPS group; P group: propofol group; L+P group: LPS+propofol group; I group: intralipid group; L+I group: LPS+intralipid group.</p

The impact of propofol on phosphorylation of p38 and the release of IL-1β, IL-6 and TNF-α under LPS stimulation after inhibition of Annexin A1 in THP-1.

<p>A. Lysis samples of THP-1 cells were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of Annexin A1. B. Gray intensity analysis of the western blot results of six groups. Each bar represents the mean ± S.D. No significant differences were found between L' group and L'+P' Group (p>0.05). L group: LPS group with wide-type Annexin A1; L+P group: LPS+propofol group with wide-type Annexin A1 ; L' group: LPS group with transfected shRNA of Annexin A1; L'+P' group: LPS+propofol group with transfected shRNA of Annexin A1. C. Lysis samples of THP-1 cells were separated on 12% SDS-PAGE gels and transferred to PVDF membranes for Western blotting analysis of phosphorylation of p38. D. Gray intensity analysis of the western blot results of four groups. Each bar represents the mean ± S.D. No significant differences were found between L' group and L'+P' Group (p>0.05). L group: LPS group with wide-type Annexin A1; L+P group: LPS+propofol group with wide-type Annexin A1 ; L' group: LPS group with transfected shRNA of Annexin A1; L'+P' group: LPS+propofol group with transfected shRNA of Annexin A1. E. the experession of mRNA of TNF-α IL-1β, IL-6 in THP-1 cells. Each bar represents the mean ± S.D, no significant differences were found L'+P' group compared with L' group.</p