30 research outputs found
Há irracionalidades no consumo de inibidores de apetite no Brasil? Uma análise farmacoeconométrica de dados em painel
Full text link013 Characterization of primary structure and microheterogeneity of fatty acid acylated lipoproteins by 252Cf-plasma desorption and electrospray mass spectrometry
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Palmitoylation of Semliki Forest virus glycoproteins in insect cells (C 6/36) occurs in an early compartment and is coupled to the cleavage of the precursor p 62
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Detection of Heterogeneous Protein S-Acylation in Cells
Get PDFThe use of synthetically synthesized azide and alkyne fatty acid analogs coupled with bioorthogonal Cu(i)-catalyzed Huisgen 1,3-dipolar cycloaddition reaction-based detection methods to study protein S-acylation reactions has replaced the traditional method of using in vivo metabolic radiolabeling with tritiated palmitic acid and has greatly facilitated our understanding of this essential cellular process. Here, we describe the chemical synthesis of myristic (C:14), palmitic (C16:0), and stearic (C18:0) acid-azide probes and detail how they may be utilized as chemical reporters for the analysis of S-acylation of exogenously expressed proteins in cells
