XENOGENEIC DERMAL MATRIX VERSUS AUTOLOGOUS CONNECTIVE TISSUE GRAFT VERSUS NO GRAFT AT ABUTMENT CONNECTION FOR IMPROVING AESTHETICS: 6-MONTH OUTCOMES OF A RANDOMISED CONTROLLED TRIAL

OBJECTIVES. The aim of this multicentre randomised controlled trial was to evaluate the efficacy of a xenogeneic dermal matrix in widening keratinised peri-implant tissues du-ring second-stage surgery, and to compare it to both autologous connective tissue graft and a control group with no augmentation. MATERIAL AND METHODS. Patients requiring an increase in keratinised gingiva width were enrolled by four university/dental practices and randomised into three different groups for grafting procedures at the implant uncovering stage: either xenogeneic dermal matrix (Group X), autologous connective tissue graft (Group A) or no graft (control, Group C). The primary outcomes were width of keratinised tissue and facial soft tissue levels, evaluated at three different time points (T0, implant uncovering stage; T1 and T2, six weeks and six months after surgery, respectively). Secondary outcomes were: implant failure, complications, marginal bone loss, papilla index, facial soft tissue level, pink esthetic score, and aesthetic assessment by patients. RESULTS. Thirty-six patients, with one implant per patient, were enrolled at two centres (18 at each centre): 12 for control, 12 for xenogeneic dermal matrix and 12 for autologous tissue graft. Three patients dropped out and two patients from the autologous group had implant failures. No complications were recorded. After six months, the width of keratini-sed tissue increased by 0.16 ± 1.01 (P = 0.79), 1.05 ± 0.76 (P = 0.01) and 0.80 ± 1.73 mm (P = 0.28), and facial soft tissue level was-0.95 ± 0.85 (P = 0.04), 0.32 ± 0.57 (P = 0.15) and 0.35 ± 0.79 mm (P = 0.30) respectively in Groups C, X and A groups. Between-group analysis showed that, with respect to control, only facial soft tissue level (1.31 mm, P = 0.01) and width of keratinised mucosa (2.43 mm, P = 0.01) outcomes in the autologous graft group were statistically significant at T2. Mean marginal bone loss between T0-T2 was-0.4 ± 0.4mm, with no differences between groups. Pink aesthetic score showed no significant differences between groups, being 0.89 for A-C (P = 0.41), 0.88 for A–X (P = 0.63) and 0.72 for X-C (P = 0.88). Patient’s aesthetic satisfaction (Visual Analogue Scale) was 92.2 ± 8.4, 93.8 ± 7.7, 97.2 ± 3.0, for Groups C, X and A, respectively. Between the two dental centres, only facial soft tissue level at T0–T2 was significantly different, by 0.67 ± 0.62 mm (P = 0.03). CONCLUSIONS. After six months, autologous connective tissue graft yielded a significant gain in facial soft tissue levels and width of keratinised mucosa, as compared to the control group (no graft)

Periodontitis Stage III–IV, Grade C and Correlated Factors: A Histomorphometric Study

Background: Periodontitis is a disease that leads to serious functional and esthetic dysfunctions. Periodontitis exists in different forms, and its etiology is related to multiple component causes. Two key processes involved in the evolution of this pathology are angiogenesis and inflammatory infiltrate. The aim of this study was to understand if important factors such as smoking, gender, age, plaque, pus, and probing pocket depth could influence the histomorphological pattern of generalized stage III-IV, grade C periodontitis (GPIII-IVC), which is a particular form of periodontitis. Methods: Eighteen subjects with GPIII-IVC were enrolled in this study. The percentage of inflammatory cells and the vascular area were measured and evaluated in relation to each periodontal disease-associated factor. Results: Females showed a significant increase in the percentage of inflammatory cells compared to males (6.29% vs. 2.28%, p-value = 0.020) and it was higher in non-smokers than in smokers (4.56% vs. 3.14%, p-value = 0.048). Young patients showed a significant increase in vascular area percentage compared to older patients (0.60% vs. 0.46%, p-value = 0.0006) and this percentage was also higher in non-smokers compared to smokers (0.41% vs. 0.55%, p-value = 0.0008). The vascular area was also more than halved in subjects with residual plaque on tooth surfaces (0.74% vs. 0.36%, p-value = 0.0005). Conclusions: These results suggested that even if these factors are commonly related to the worsening of periodontal status, some of them (pus and periodontal probing depth (PPD)) do not affect the inflammatory and vascular patterns