Preliminary research on the operation mode of virtual-real integration in fully-mechanized mining face based on industrial metaverse

The key to promoting intelligent construction is to integrate the digital twin technology form the operation mode of virtual and real integration. And the industrial metaverse based on digital twin is the future development direction of intelligent mining face. The concept of virtual and real integration operation mode of fully-mechanized mining face based on virtual reality-digital twin-cyber physical system-industrial metaverse is proposed. It has six connotation characteristics, such as display and off-line simulation, monitoring and auxiliary operation, online simulation and preview. It is an evolution process from low-level display simulation to high-level deep integration function. Finally, it have four abilities : the ability of reproduction mapping from real to virtual precision, the ability of reasoning and forecasting decision-making in virtual iteration, the ability of reproduction control from virtual to real, the ability of seamless cooperation between virtual and real human-computer, and the ability of lean management. The four capabilities of industrial metaverse and the key technologies to realize industrial metaverse are analyzed.Based on the existing monitoring, decision-making and control capabilities, AR remote assistance technology that can strengthen the cooperation ability between field operators and remote operators, robot cooperation technology that can strengthen the safety of operators, and virtual human technology that can use AI-driven operation in virtual space are integrated to build a hydraulic support adjusting experimental system based on industrial metaveise,and preliminary understanding of the application of industrial metaverse in coal mining

The molecular mechanism of ferroptosis and its role in COPD

Ferroptosis, a new type of cell death, is mainly characterized by intracellular iron accumulation and lipid peroxidation. The complex regulatory network of iron metabolism, lipid metabolism, amino acid metabolism, p53-related signaling, and Nrf2-related signaling factors is involved in the entire process of ferroptosis. It has been reported that ferroptosis is involved in the pathogenesis of neurological diseases, cancer, and ischemia–reperfusion injury. Recent studies found that ferroptosis is closely related to the pathogenesis of COPD, which, to some extent, indicates that ferroptosis is a potential therapeutic target for COPD. This article mainly discusses the related mechanisms of ferroptosis, including metabolic regulation and signaling pathway regulation, with special attention to its role in the pathogenesis of COPD, aiming to provide safe and effective therapeutic targets for chronic airway inflammatory diseases

lncRNA LOC100911717-targeting GAP43-mediated sympathetic remodeling after myocardial infarction in rats

ObjectiveSympathetic remodeling after myocardial infarction (MI) is the primary cause of ventricular arrhythmias (VAs), leading to sudden cardiac death (SCD). M1-type macrophages are closely associated with inflammation and sympathetic remodeling after MI. Long noncoding RNAs (lncRNAs) are critical for the regulation of cardiovascular disease development. Therefore, this study aimed to identify the lncRNAs involved in MI and reveal a possible regulatory mechanism.Methods and resultsM0- and M1-type macrophages were selected for sequencing and screened for differentially expressed lncRNAs. The data revealed that lncRNA LOC100911717 was upregulated in M1-type macrophages but not in M0-type macrophages. In addition, the lncRNA LOC100911717 was upregulated in heart tissues after MI. Furthermore, an RNA pull-down assay revealed that lncRNA LOC100911717 could interact with growth-associated protein 43 (GAP43). Essentially, immunofluorescence assays and programmed electrical stimulation demonstrated that GAP43 expression was suppressed and VA incidence was reduced after lncRNA LOC100911717 knockdown in rat hearts using an adeno-associated virus.ConclusionsWe observed a novel relationship between lncRNA LOC100911717 and GAP43. After MI, lncRNA LOC100911717 was upregulated and GAP43 expression was enhanced, thus increasing the extent of sympathetic remodeling and the frequency of VA events. Consequently, silencing lncRNA LOC100911717 could reduce sympathetic remodeling and VAs

Study on SO

To study the effect of types of boilers, pollutant treatment facilities and coal quality on SO3, emission concentration of SO3 was tested by controlled condensation method for seven typical ultra-low emissions coal-fired units in western region of Inner Mongolia. The test results showed that the SO3 emission concentration of all units ranged from 0.36 mg/m3 to 2.04 mg/m3, much lower than 5 mg/m3, so there was no need to add SO3 removal facilities. For the units burning the coal of same sulfur content, which adopt LLTESP and WESP has the lower SO3 emission concentration. The SO3 emission concentration can also be controlled at a lower value when SNCR and double-tower double-cycle WFGD technology are adopted in the unit burning medium-high sulfur coal

Analysis on the Effect of Groundwater Overexploitation Control in Water receiving region of the First Phase of the South-North Water Transfer Project

The South-North Water Transfer Project is playing a more and more important role in ensuring economic and social development and maintaining a good ecological environment for north of China. However, long-term over-exploitation of groundwater has caused a series of ecological and environmental problems. The first phase of the South-to-North Water Transfer Project was successfully passed through in 2013 and 2014. The water supplied by this huge project provided critical water source for implementing groundwater overexploitation control. In order to promote the management and protection of groundwater resources, the overdraft areas had adopted comprehensive measures to reduce groundwater extraction, such as accelerating the construction of supporting projects, shutting down groundwater mining wells, improving the groundwater monitoring station network, and reforming the water resources fees and so on. The urban groundwater overexploitation control work has received good progress. Based on the investigation and statistics of groundwater overexploitation control in the water receiving region of the first phase of the South-North Water Transfer Project, it was found that since the first phase of the South-to-North Water Transfer Project passing though, the water groundwater withdrawal decreased by 15.23×108 m3 by making full use of the water from the South-North Water Transfer Project, including 2.36×108 m3 in Beijing, 0.67×108 m3 in Tianjin, 6.39×108 m3 in Hebei, 3.84×108 m3 in Henan, 1.62×108 m3 in Shandong, and 0.35×108 m3 in Jiangsu, respectively. The number of groundwater withdrawal wells closed was 15202, including 331 wells in Beijing, 582 in Tianjin, 4895 in Hebei, 6213 in Henan, 2012 in Shandong, and 1169 eyes in Jiangsu, respectively. In terms of groundwater level, the trend of continuous decline in groundwater level has been effectively curbed in most areas of the water receiving region, however, in some areas the groundwater level is still declining due to the too large cumulative over-exploitation of groundwater. Shijiazhuang City was selected as typical monitoring site to explain the groundwater overexploitation control effect on groundwater level. The analysis of the monitoring data of typical monitoring sites showed that groundwater overexploitation control has a great influence on the groundwater level change in Shijiazhuang urban area. This study also puts forward some problems and suggestions in promoting the groundwater overexploitation control in the water receiving region, and provides reference for the construction of ecological civilization and national water security

miRNA-21, which disrupts metabolic reprogramming to facilitate CD4+ T cell polarization toward the Th2 phenotype, accelerates arsenite-induced hepatic fibrosis

Elevated levels of arsenic may be present in groundwater, and long-term exposure to arsenic increases hepatic fibrosis. T helper 2 (Th2) cells are involved in the fibrotic cascade, and cell metabolism is a regulatory factor participating in CD4+ T cell differentiation and function. However, the mechanism for Th2 cell regulation of arsenite-induced hepatic fibrosis is not fully understood. In present study, for arsenite-fed mice, activated hepatic stellate cells may be involved in the infiltration of CD4+ T cells, accompanied by up-regulation of GATA3, a transcription factor, and IL-13, the major Th2 cytokine. Exposed to arsenite, Jurkat cells had increased aerobic glycolysis to promote the cell cycle and cell proliferation. Further, this process elevated levels of marker molecules, including those of the Th2 paradigm characterized by GATA3, IL-4, and IL-13. LX-2 cells were activated when treated with culture medium from Jurkat cells exposed to arsenite. miR-21 may be a therapeutic target for arsenite-induced hepatic fibrosis. In vitro, miR-21 knock-down caused inhibition of the PTEN/PI3K/AKT pathway induced by arsenite. It also reversed the elevated glycolysis and the accelerated cell cycle and cell proliferation. Indeed, this alteration led to diminished expression of GATA3, IL-4, and IL-13 in T cells differentiated under Th2 conditions, which inhibits activation of LX-2 cells. Consistent with the results in vitro, miR-21 knock-out in mice reversed hepatic fibrosis and attenuated the levels of GATA3 and IL-13 induced by arsenite. These findings indicate that miR-21 regulates the glycolysis of CD4+ T cells through the PTEN/PI3K/AKT pathway to accelerate the cell cycle, thereby facilitating CD4+ T cell polarization toward Th2 and releasing the fibrogenic factor IL-13, which participates in arsenite-associated hepatic fibrosis. Inhibition of Th2 polarization of CD4+T cells or miR-21 could be a therapeutic strategy to combat hepatic fibrosis caused by exposure to arsenic

Molecular authentication of the traditional Chinese medicine Tongren Dahuoluo Wan and its alternative formulation

Tongren Dahuoluo Wan has been a popular traditional Chinese medicine in international pharmaceutical markets for hundreds of years. Leopard bone powder is the key element in its formulation. However, the leopard has been listed for wildlife conservation, which limits the use of the leopard bone supplies. Therefore, an alternative formulation which substitutes leopard bone with zokor bone in the formula of Tongren Dahuoluo Wan is now manufactured. To develop a simple and reliable molecular method for authenticating the two patent medicines, mitochondrial nucleotide polymorphic sites of 12S rRNA, COI and Cytb genes were screened in leopard and zokor bones, and nine pairs of species-specific primers were verified for discriminating the two species. For the patent medicine authentication, we set up a molecular diagnostic assay to resolve the difficulties of low concentration of target DNAs and presence of PCR-inhibitory substances in this complex medicine, and successfully confirmed leopard or zokor content using the nine pairs of species-specific primers. We recommend a common technical strategy for authentication of species origins in traditional Chinese medicine, and discuss the experimental solutions for technical problems of molecular diagnostic assays

Effects of intracoronary low-dose prourokinase administration on ST-segment elevation in patients with myocardial infarction and a high thrombus burden: a randomized controlled trial

Objective To evaluate the efficacy and safety of low-dose prourokinase (pro-UK) administration during primary percutaneous coronary intervention (PCI) for the treatment of acute ST-segment elevation myocardial infarction (STEMI) in patients with a high thrombus burden. Methods A prospective, randomized controlled trial was conducted at the Inner Mongolia People’s Hospital, China. Patients with STEMI and a high thrombus burden who underwent thrombus aspiration and primary PCI were randomly allocated to pro-UK administration or control groups. The primary endpoint was corrected thrombolysis in myocardial infarction (TIMI) frame count (CTFC). Results There were no significant differences in the baseline demographics or clinical characteristics of the two groups. The CTFC, tissue myocardial perfusion grade, ST-segment resolution, and myocardial blush grade of the pro-UK group were significantly better than those of the control group. In addition, after 30 days of follow-up, the pro-UK group had better cardiac function and perfusion than the control group. There were no differences in the clinical outcomes or incidence of hemorrhage. Conclusions Intracoronary low-dose pro-UK improves myocardial perfusion and cardiac function in patients with a high thrombus burden. Major hemorrhages still occur in patients administered pro-UK, but are no more frequent. Study registration : Chinese Clinical Trial Registry (ChiCTR1900022290)