43 research outputs found
Conservatively treated glassy cell carcinoma of the cervix
<p>Abstract</p> <p>Background</p> <p>Very little data about the conservative treatment of early stage glassy cell cervical cancer have been reported.</p> <p>Case presentation</p> <p>A 30-year old patient, nulligravida was admitted to the Gynecologic Oncology Unit of the Catholic University of Campobasso for irregular post-coital vaginal bleeding. The patients was staged as having FIGO stage IB1 (tumor diameter = 2 cm) squamous cervical cancer. After extensive counseling of the patient and her family, laparoscopic pelvic lymphadenectomy and cold knife conization were performed. The final diagnosis was FIGO Stage IB1 glassy cell carcinoma. Currently, after a follow-up of 38 months, she has no evidence of disease.</p> <p>Conclusion</p> <p>We reported a case of early stage glassy cell cancer patient, who was conservatively treated by conization and laparoscopic pelvic lymphadenectomy.</p
Standard model contribution to the electric dipole moment of the deuteron, 3H, and 3He nuclei
Specific Variants in the MLH1 Gene Region May Drive DNA Methylation, Loss of Protein Expression, and MSI-H Colorectal Cancer
Background: We previously identified an association between a mismatch repair gene, MLH1, promoter SNP (rs1800734)
and microsatellite unstable (MSI-H) colorectal cancers (CRCs) in two samples. The current study expanded on this finding as
we explored the genetic basis of DNA methylation in this region of chromosome 3. We hypothesized that specific
polymorphisms in the MLH1 gene region predispose it to DNA methylation, resulting in the loss of MLH1 gene expression,
mismatch-repair function, and consequently to genome-wide microsatellite instability.
Methodology/Principal Findings: We first tested our hypothesis in one sample from Ontario (901 cases, 1,097 controls) and
replicated major findings in two additional samples from Newfoundland and Labrador (479 cases, 336 controls) and from
Seattle (591 cases, 629 controls). Logistic regression was used to test for association between SNPs in the region of MLH1
and CRC, MSI-H CRC, MLH1 gene expression in CRC, and DNA methylation in CRC. The association between rs1800734 and
MSI-H CRCs, previously reported in Ontario and Newfoundland, was replicated in the Seattle sample. Two additional SNPs, in
strong linkage disequilibrium with rs1800734, showed strong associations with MLH1 promoter methylation, loss of MLH1
protein, and MSI-H CRC in all three samples. The logistic regression model of MSI-H CRC that included MLH1-promotermethylation
status and MLH1 immunohisotchemistry status fit most parsimoniously in all three samples combined. When
rs1800734 was added to this model, its effect was not statistically significant (P-value = 0.72 vs. 2.361024 when the SNP was
examined alone).
Conclusions/Significance: The observed association of rs1800734 with MSI-H CRC occurs through its effect on the MLH1
promoter methylation, MLH1 IHC deficiency, or both