72 research outputs found

    ショシン シンリ リンショウカ ニオケル セラピスト フォーカシング ノ イミ

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    心理臨床家にとって、自分自身の心理過程を検討することはクライエントを理解することと同様に極めて重要である。「セラピスト・フォーカシング」はセラピストがある事例を担当するうえでの自分自身のフェルトセンスに触れることによって、体験過程の推進が生じるのを促す方法である。本研究は、セラピスト・フォーカシングの1 セッションを提示し、初心心理臨床家にとってのセラピスト・フォーカシングの意義について、セラピストは「何を」「どのように」してその過程で体験していくかを考察し、その上でセラピスト・フォーカシングとスーパービジョンの組み合わせについて検討した。その結果、セラピスト・フォーカシングがフォーカサーとガイドとの2 者関係の相互作用によって事例の理解を促すという点と、セラピスト・フォーカシングによりセラピストが体験過程に触れることにより、スーパービジョンに主体的に望むことができるという点が示唆された。It is extremely important for psychotherapists to examine their own psychological process as well as understanding that of the client. “Therapist focusing” is a method of urging the promotion of the experience process caused by touching the charge of the case with the therapist and own felt sense. One session of the therapist focusing was presented, and the therapist considered whether to do “What” and “How” and to experience in the process, and examined the present study about the combination of the therapist focusing and the supervision about the meaning of the therapist focusing for the Beginner psychotherapists on that. As a result, two points were suggested. The first point is that the understanding of the interaction related to focuser in therapist focusing and two people with the guide urges the understanding of the case. It is possible to hope to the supervision independently in the second point because the therapist touches the experience process in therapist focusing.研究論

    リンショウ シンリ ジッシュウ ニオケル セラピスト フォーカシング ノ イミ

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    It is extremely important for the psychotherapists to examine own psychologicalprocess as well as understanding the client. “Therapist focusing” is amethod of urging promotion of the experience process to be caused by touchingthe charge of the case with the therapist and own felt sense. One session of thetherapist focusing was presented, the meaning of “Therapist focusing” in beginnerpsychotherapist” “Clinical psychology training” was examined by turningaround by the talk form. As a result, the meaning of therapist focusing as experiencein which the self-understanding and they in therapist focusing receivepsychotherapy was suggested

    Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes

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    A full-length cDNA coding for a putative adenosine deaminase (Fv-ada) was isolated from the basidiomycete Flammulina velutipes. Fv-ada encodes a polypeptide consisting of 537 amino acid residues, which has a consensus sequence conserved among adenosine deaminase-related growth factors (ADGF) found in several metazoa, including chordates and insects. Fv-ada transcript was detected at all stages of growth in dikaryotic F. velutipes cells, with a peak at the primordial stage. Heterologous expression of Fv-ada in the yeast Pichia pastoris produced recombinant Fv-ADA that catalyzed the conversion of adenosine to inosine. Dikaryotic mycelia from F. velutipes were transformed with the binary plasmid pFungiway-Fv-ada, which was designed to suppress the expression of Fv-ada through RNA interference. The growth rates of the resulting transformants were retarded in response to the degree of suppression, indicating that Fv-ada plays an important role in the mycelial growth of F. velutipes. These results suggested that ADGF could function as growth factors in fungi, as is seen in other eukaryotes.ArticleJOURNAL OF BIOSCIENCE AND BIOENGINEERING. 115(4):360-365 (2013)journal articl

    シコウ カウンセリング ニオケル セラピスト フォーカシング ノ イミ

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    It is extremely important for the psychotherapists to examine own psychological process as well as understanding the client. “Therapist focusing” is a method of urging promotion of the experience process to be caused by touching the charge of the case with the therapist and own felt sense. One session of the therapist focusing was presented, the meaning of “Therapist focusing” in beginner psychotherapist “Trial counseling” was examined by turning around by the talk form. As a result, two points were suggested. the first point is that the process of “Close examination of the felt sense” of the therapist focusing is approximated with the understanding of the counter transference and relativity of “Therapist focusing” and assessment of client

    Subsurface velocity structure and site amplification characteristics in Mashiki Town, Kumamoto Prefecture, Japan, inferred from microtremor and aftershock recordings of the 2016 Kumamoto earthquakes

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    In order to investigate the seismic velocity structure in the region of concentrated severe damage during the 2016 Kumamoto earthquake sequence, we conducted continuous seismic observations in the central area of Mashiki Town, Kumamoto Prefecture. During 4 days of observations at eight temporary sites, 2 months after the mainshock, recordings from 30 aftershocks (1.7 ≤ Mj ≤ 4.3, 1.9 km ≤ depth ≤ 13.5 km) were obtained. The aftershock data showed that site amplifications at approximately 1 Hz are dominant in a zone where almost no buildings were damaged along the Akitsu riverside, whereas site amplifications at higher than 3 Hz are observed in the heavily damaged zones. Our data also showed that the peak acceleration and velocity amplitudes, as well as seismic intensities for the small events in the less damaged zone, are clearly larger than those in the damaged zones, implying that the damage distribution is inconsistent with site response based on linear site amplifications. The estimated phase velocities of Rayleigh waves using the aftershock and microtremor data show dispersive characteristics in the lower frequency range (0.26 ≤ f ≤ 1.27 Hz), but the values are substantially smaller than those derived from the P–S logging model at the nearest KiK-net strong-motion observation station KMMH16. The derived microtremor horizontal-to-vertical spectral ratios and earthquake radial-to-vertical (R/V) spectral ratios show common distinct peaks at around 0.4 Hz, which are possibly related to the response of deep sedimentary layers beneath the area. The refined velocity structure model that better accounts for both the phase velocity and common dominant peak indicates that the values of S wave velocity (Vs) above the bedrock layer (Vs = 2700 m/s) are smaller than those inferred from the logging model and the depth to the bedrock layer could be much deeper (about 600 m) in comparison with the logging model (234 m). The derived R/V spectral ratio at station KMMH16 also shows a distinct peak at 0.4 Hz, suggesting that there is no large difference of deep sedimentary structure between the observation area and station KMMH16

    Characterization of a gene coding for a putative adenosine deaminase-related growth factor by RNA interference in the Basidiomycete Flammulina velutipes

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    A full-length cDNA coding for a putative adenosine deaminase (Fv-ada) was isolated from the basidiomycete Flammulina velutipes. Fv-ada encodes a polypeptide consisting of 537 amino acid residues, which has a consensus sequence conserved among adenosine deaminase-related growth factors (ADGF) found in several metazoa, including chordates and insects. Fv-ada transcript was detected at all stages of growth in dikaryotic F. velutipes cells, with a peak at the primordial stage. Heterologous expression of Fv-ada in the yeast Pichia pastoris produced recombinant Fv-ADA that catalyzed the conversion of adenosine to inosine. Dikaryotic mycelia from F. velutipes were transformed with the binary plasmid pFungiway-Fv-ada, which was designed to suppress the expression of Fv-ada through RNA interference. The growth rates of the resulting transformants were retarded in response to the degree of suppression, indicating that Fv-ada plays an important role in the mycelial growth of F. velutipes. These results suggested that ADGF could function as growth factors in fungi, as is seen in other eukaryotes.ArticleJOURNAL OF BIOSCIENCE AND BIOENGINEERING. 115(4):360-365 (2013)journal articl

    Visual Properties of Transgenic Rats Harboring the Channelrhodopsin-2 Gene Regulated by the Thy-1.2 Promoter

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    Channelrhodopsin-2 (ChR2), one of the archea-type rhodopsins from green algae, is a potentially useful optogenetic tool for restoring vision in patients with photoreceptor degeneration, such as retinitis pigmentosa. If the ChR2 gene is transferred to retinal ganglion cells (RGCs), which send visual information to the brain, the RGCs may be repurposed to act as photoreceptors. In this study, by using a transgenic rat expressing ChR2 specifically in the RGCs under the regulation of a Thy-1.2 promoter, we tested the possibility that direct photoactivation of RGCs could restore effective vision. Although the contrast sensitivities of the optomotor responses of transgenic rats were similar to those observed in the wild-type rats, they were enhanced for visual stimuli of low-spatial frequency after the degeneration of native photoreceptors. This result suggests that the visual signals derived from the ChR2-expressing RGCs were reinterpreted by the brain to form behavior-related vision

    Id4, a New Candidate Gene for Senile Osteoporosis, Acts as a Molecular Switch Promoting Osteoblast Differentiation

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    Excessive accumulation of bone marrow adipocytes observed in senile osteoporosis or age-related osteopenia is caused by the unbalanced differentiation of MSCs into bone marrow adipocytes or osteoblasts. Several transcription factors are known to regulate the balance between adipocyte and osteoblast differentiation. However, the molecular mechanisms that regulate the balance between adipocyte and osteoblast differentiation in the bone marrow have yet to be elucidated. To identify candidate genes associated with senile osteoporosis, we performed genome-wide expression analyses of differentiating osteoblasts and adipocytes. Among transcription factors that were enriched in the early phase of differentiation, Id4 was identified as a key molecule affecting the differentiation of both cell types. Experiments using bone marrow-derived stromal cell line ST2 and Id4-deficient mice showed that lack of Id4 drastically reduces osteoblast differentiation and drives differentiation toward adipocytes. On the other hand knockdown of Id4 in adipogenic-induced ST2 cells increased the expression of Pparγ2, a master regulator of adipocyte differentiation. Similar results were observed in bone marrow cells of femur and tibia of Id4-deficient mice. However the effect of Id4 on Pparγ2 and adipocyte differentiation is unlikely to be of direct nature. The mechanism of Id4 promoting osteoblast differentiation is associated with the Id4-mediated release of Hes1 from Hes1-Hey2 complexes. Hes1 increases the stability and transcriptional activity of Runx2, a key molecule of osteoblast differentiation, which results in an enhanced osteoblast-specific gene expression. The new role of Id4 in promoting osteoblast differentiation renders it a target for preventing the onset of senile osteoporosis
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