Possibility of Sand Culture for Melon Using Root-proof Capillary Wick in Mid-summer Period

Effects of liquid fertilizer (LF) and controlled-release fertilizer (CRF) on growth and fruit enlargement of netted melon in 6L sand medium were investigated in a cultivation method using root-proof capillary wicks in mid-summer. Average of maximum and minimum root-zone temperatures was 34.8°Cand 26.9°C, respectively. Wilted symptoms appeared very often in 5 out of 8 plants at 2 weeks after transplanting in CRF with a high electric conductivity (EC) of medium solution. In contrast, such symptoms were not found at all in LF with a lower EC. In LF, average fruit weight was 2.3Kg and fruit Brix was 14.4, and non-wilted plants in CRF also showed the same levels as LF in weight and Brix of fruits. These results indicated the possibility of sand culture for melon in mid-summer with LF or a modified fertilizer combination design of CRF.栽培期間の大半の日数が高温となる盛夏期において防根給水ひもを用いたメロン砂栽培が可能であるかどうかを検討した．処理区は，大塚A処方培養液のECを段階的に低下させて供給する培養液区，全量を肥料調節型肥料で培地に混和する肥効調節型肥料区（以下、肥料区）の2区とした．最高平均地温は34.8℃，最低平均地温は26.9℃であった．肥料区では定植2週間後の交配期に8株中5株に萎れ症状が生じたが，これは定植後～交配期において培地が高EC状態であったことが原因と考えら れた．一方で，培養液区では萎れ症状は全くみられなかった．肥料区の正常３個体と培養液区の全個体の果実重の平均は2.3 ㎏，糖度 (Brix°) は14.4であった．以上より，盛夏期における防根給水ひもを用いたメロン砂栽培は，培養液管理によって安定生産が見込めること，肥料での栽培も施肥設計を考慮すれば同期の砂栽培に適用可能であることが示唆された

Movement of Calcuium (45Ca) to Fruit from Peduncle of Tomato Plants

Movement of calcium (45Ca) from the peduncle to fruit of tomato during the days and night was investigated. Further, the effects of additional NaCl to the culture solution on the water potential in plants and 45Ca movement to fruit were also examined. The activity of 45Ca on a dry weight basis in the peduncle was higher during the day than at night, however, in calyx and fruit the 45Ca activity was similar both during the day and night. Import of 45Ca into the leaves from the peduncle was not different between the day and the night. Additional NaCl to the culture solution resulted in a greater water saturation deficit (WSD) of the immature leaves just below the 2nd and 3rd trusses, unlike with the mature leaves below the 1st truss where the WSD did not decrease with NaCl treatment. On the oher hand, the activity of 45Ca in the peduncle and calyx was not different between the NaCl treatment and the control. However, the activity in the distal part of the fruits was lower with, NaCl treament than in the control. These results suggested that the high WSD effectively decreased the import of calcium into fruits from the peduncle. It seems that the import of 45Ca into fruit not only dependent on the conductive tissues but also a possible driving force in xylem water flow, and that NaCl interferes with movement of calcium in the fruits.トマト果柄に木綿糸を通してカルシウム(45Ca)を処理し昼夜間別に果房各部位への45Caの移行、ならびに、培養液にNaClを添加した場合の樹体内の水分状態と果実への45Ca移行について調査した。昼夜間における単位乾物重当りの45Caの非活性は果柄で昼間に有意に高くなったが、がく・果実では昼夜間差は生じなかった。また、葉でも昼夜間差はなく昼夜同程度に45Caの移行が認められた。培養液にNaClを添加しても、第1果房直下葉(成熟葉)の水分欠差が生じなかったが、第2･3段果房直下葉(未成熟葉)ではNaCl添加のよって水分欠差は有意に増大し、果柄・がくの45Ca非活性も高くなる傾向を示した。しかし果実末端部の45Ca非活性は有意に低くなり、同時に、果実全体に占める果実末端部での45Ca非活性もNaCl添加によって減少した。本試験の結果から、木綿糸処理液の果柄内への流入は樹体の水分欠差に影響を受けると考えられた。果柄内に流入した45Caは、水の流れだけでなく他の要因によっても各部位に移行するものと思われた。また、培養液へのNaCl添加により、果実末端部へのカルシウム移行がより強く抑制されるのは、果実内でのカチオン移動に競合が生じるからではないかと推察された

Effect of Controlled-release Fertilizer and Root-proof Capillary Wick Addition to Root-zone on Tomato Growth and Yield

This study was conducted to investigate the effects of controlled-release fertilizers and wick addition to the root-zone on plant growth and yield of medium-fruit sized tomato up to the 18(th) truss in long-term forcing culture. The set-up consisted of a box partitioned by a board into two halves, named box 1 and box 2. Box 1 contained 2.8 ℓ of growth medium (soil : bark : perlite : peat=2 : 2 : 1 : 1). Plant was grown in box 1 with a capillary wick, and at flowering of the 8(th) truss, the partition was removed and box 2 filled with 2.8 ℓ of the medium. There were two treatments, with or without capillary wick in box 2, namely, single wick (S) and double wicks (D). Plant height was greater in D than S in January, and the difference increased gradually thereafter. Flowering time of 18(th) truss in D was 10 days earlier and decapitated shoot weight was twice that of S. Fruit yield per plant was 8 kg in S and 9 kg in D with similar value of Brix and titratable acidity. There was no difference between S and D in dry root weight (7g/plant) or in xylem exudates (8ml/h), while in S in April plant growth was inferior, leaf color yellow greenish and fruit colour uneven towards the end of growth of plants. Xylem sap analysis showed that NO(3)-N was 10 me/ℓ in S and 6 me/ℓ in D. This concentration is weaker than that of Enshi standard nutrient solution generally used in hydroponics. These results suggest that application of controlled-release fertilizers and wick addition to box 2 with root-zone extended was effective for plant growth performance and fruit yield.中玉トマト18段摘心の長期促成栽培における肥効調節型肥料の適用と生育途中の根域拡張に伴う「紐」適用が生育と果実収量に及ぼす効果を調べた．栽培容器は可動仕切り板で2つに仕切り区画当たり容量は2.8ℓとし，第8段花房開花時に仕切り板をはずして5.6ℓ（1st Box＋2nd Box）とした．試験区は2区で2nd Boxに紐を配置しない1本区と2nd Boxにもう１本を配した2本区である．2本区の草丈は1月以降，1本区に比べ高く推移し第18段花房の開花日が約10日早まり，成長の指標とした摘心時の茎頂部新鮮重も1本区の2倍となった．1株の果実収量は1本区で8kg，2本区で9kgとなり果実の糖度，酸度には両区間に差はなかった．栽培終了時の根の乾物重と茎切断面から採取した木部いっ泌液は両区間に差はなかったが，両区とも栽培終期の4月には生育が衰え葉色は黄緑色で果実の着色も不均一であった．木部いっ泌の成分分析ではNO(3)-N濃度が1本区で10me/ℓ，2本区で6me/ℓであり，これらの濃度は一般に養液栽培に使用される園試標準濃度よりも低かった．以上の結果から，第8段花房開花時に仕切りを開放し新区画への土壌と肥料ならびに紐の新たな適用は，生育安定と収量確保に効果的に作用することが明らかとなった．しかし，紐の有無にかかわらず生育後期の生育が不良となったことから，今後は施肥設計の面から，とくにNO(3)-NとSO(4)-Sの両面から検討する必要があると考察した

Pericarp Characteristics of the F(1) Hybrid Medium-Fruited Tomato between the Male Sterile Mutant (T-4) of the Large-Fruited 'First' and a Small-Fruited Pure Line with Soft Pericarp

Breeding for a soft pericarp in medium-sized tomato fruit was conducted by crossing the male sterile mutant (T-4) of the large-fruited 'First' and a small-fruited pure line with a soft pericarp (S). Pericarp characteristics of the F(1) hybrid (named MS-II) were compared with the parents and two similar medium-fruited tomato cultivars, 'Red ore' and 'Frutica'. Pericarp firmness in MS-II was lower as compared with that of both T-4 and S. Differences in pericarp firmness among MS-II, 'Red ore' and 'Frutica' were dependent on truss. In the first truss, MS-II developed fruits with a softer pericarp than 'Red ore', but with a firmer pericarp than 'Frutica'. In the second and third trusses, pericarp firmness of the fruit in MS-II tended to be lower than those of the other two cultivars. The thickness of the exocarp cuticle in MS-II was lower than that in 'Red ore', but was no different to that in 'Frutica'. Thus genotypic differences in pericarp firmness among MS-II, 'Red ore' and 'Frutica' seem to be derived from differences in the degree of cutin development in the epidermal perimeter. A thinner cuticle can explain pericarp softness in the fruits above the second truss in MS-II.薄皮果柔の中玉トマト品種育成を目的とし，‘ファースト’花粉非崩壊型雄性不稔系統 (T-4) を種子親，果皮の軟らかい小果固定系統 (S) を花粉親とするF(1)雑種 (MS-II) の特性について，両親系統および既存の中玉F(1)品種‘レッドオーレ’，‘フルティカ’のそれと比較した．両親系統と比較したところ，MS-IIの果実硬度は花粉親である軟果皮Sと同等となり，果皮硬度はSよりも低い値となった．3段摘心栽培において，MS-IIの果実硬度は第1段では2品種と差は無かったが，上位果房ほど両品種よりも低くなる傾向を示した．果皮硬度は，第1果房では‘レッドオーレ’よりも低く，‘フルティカ’よりも高かったが，上位果房ほど両品種よりも低い値となる傾向を示した．MS-IIの外果皮におけるクチクラ厚を測定したところ，‘フルティカ’と同等となり，‘レッドオーレ’よりも低かった．また，MS-IIのクチクラ層の発達程度が2品種よりも低いことが観察され，MS-IIの果皮硬度が2品種よりも低い傾向を示すのは，外果皮におけるクチクラ層の発達程度が低いためと推測された

Application of Controlled-release Fertilizer to Netted Melon Cultivation Using Root-proof Capillary Wick

Use of controlled-release fertilizer in limited amount of soil and sand medium for netted melon cultivation using a root-proof capillary wick was investigated. The first experiment consisted of 3 lots of soil medium. 1) 3L medium used throughout the cultivation period (A); 2) 6L medium used throughout the cultivation period (B); 3) 3L used until pollination stage, then a partitioning board removed to increase the growth medium to 6L (C). Average of one fruit weight was 1.4 kg in A and B, but only 1.2 kg in C. Brix° value was 13.1 in A, 13.6 in B and 13.4 in C, respectively. The stem diameter at the time of pollination was largest in B in which there was fruit cracking that was also observed in C, but not in A. The root dry weight was highest in A. These results indicate that the restricted medium of 3L presents a potential for good fruit production. The second experiment consisted of 4 lots with 3L medium each: 1) soil and fertilizer medium as in experiment 1; 2) sand and fertilizer medium as in lot 1); 3) sand and slower release fertilizer; 4) sand and half of fertilizer at planting and an additional fertilizer bag placed on the wick at time of pollination. Although cracking fruits occurred 75% in 3) and 37.5% in 4), there was no cracking of fruits in 1) and 2), in which fruit weight in the latter was 1.5kg and brix° value of 14.5 was obtained. These results indicate that controlled-release fertilizer is useful for fruit production of netted melon in sand medium of 3L with a high potential for reuse

トマト’ファースト’の雄性不稔突然変異系統における花粉崩壊のジベレリンによる部分的阻害

The young flower buds in three male sterile mutants derived from cv.First were treated with gibberellins to determine whether pollen degradation could be inhibited.Microscopic observations of acetocarmine preparations revealed that the application of gibberellins inhibitde pollen degragation for all tested mutants of Fms-1,Fms2 and Fms-3 in which their breakdown in microsporogenesis occurs at microspore stage,during meiosis and at tetrad formation ,respectively.The optimum concentration and treatment time were 10mg/1 and 6-12days before flowering,GA7 proved to be preferable to GA3 for pollen fertility restoration.With the elingation of stameans by gibberellin application,viable pollens appeared frequeitly in Fms-2 mutant,being over 200grains on a 0.5mm equatorial section of anthers in the best case,as compared to the other mutants.Bioassay using dwafrice seedilings showe that the Fms-2 plants had lower levels of gibberellin in their anthers compared with normal ones.トマト’ファースト’より作出した雄性不稔系統において、その花粉崩壊がジベレリンによって阻害されるかどうかを検討した。ジベレリンの阻害効果は花粉崩壊が小胞子期に始まるFms-1タイプ、減数分裂期に始まるFms-2タイプ、四分子期に始まるFms-3タイプのいずれにおいても認められた。最適濃度は10mg/ℓで散布後6日から12日目に開花してくる葯で認められた。この場合、GA3よりもGA7の方が効果は高かった。開花時の葯0.25mm横断切片上のアセトカーミン染色花粉（生存花粉）はFms-2で最も多く200粒以上を示すものもあった。Fms-2花粉の生存には葯の委縮回復が伴っていた。Fms-2葯のジベレリン活性はオリジナル正常葯のそれより低かった

Large-fruited Tomato Production as Affected by Root-zone Extension and Wick Addition During Cultivation in a Capillary Wick System

This study was conducted to investigate the possibility to use capillary wick system in large-fruited tomato production. The first experiment in the autumn-winter season of 2006 was carried out to investigate the effects of the amount of substrate on growth and yield of tomato. Treatments involved growing large-fruited tomato in one box (2.8ℓ/plant) continuously, or adding substrate at flowering of the 7th truss in a 2nd box (5.6ℓ/plant). Fruit yield was higher when the root-zone was extended with double the substrate volume. The second experiment in spring-summer season of 2007 was aimed at improving growth and yield of tomato by extension of root-zone and addition of wick. Tomato plants were either grown in one box with one wick continuously, or with addition of substrate in a 2nd box at flowering of the 4th truss. Additionally, one more wick was inserted into 50% of the 2nd boxes. Half of the plants were grown in two boxes with one wick, and the other half with two wicks. Higher yield was obtained from the plants grown in two boxes with two wicks, suggesting that fruit yield was increased by increasing water transport through wick addition coupled with root-zone extension. Plant growth and fruit yield of large-fruited tomato was stable without blossom-end rot when root-zone was extended and half strength of Ohtsuka-A nutrient solution supplied through the capillary wick system. There was, however, a slight sign of physiological disorder at the leaf margins similar to potassium deficiency.「防根給水ひも」を利用した底面給水法が大玉トマト生産に適用可能かどうかを検討した．2006年秋ﾝ冬季の実験では，培地量がトマトの生育と収量に及ぼす影響について調査した．大玉トマトを終始1stBox（＝2.8ℓ･plant－1）で栽培する処理区と７段花房開花時に2ndBox（＝2.8ℓ）に培地を追加し，最終的に１stBox + 2ndBox（＝5.6ℓ･plant－1）で栽培する処理区を比較した結果，培地量を２倍にし，根域を拡張した処理区の収量が高くなった．2007年春ﾝ夏季の実験では根域拡張および「ひも」適用本数の増加によりトマトの生育収量を改善出来るかを検討するため，終始 1stBox，「ひも」1本で栽培する区，4段花房開花時に培地を追加し最終的に1stBox + 2ndBoxで栽培するが，そこには「ひも」は配さない区，培地を加えると同時に「ひも」を配する区の3区でトマトを栽培した．その結果，1stBox + 2ndBox，「ひも」2本で栽培したトマトが最も収量が高かった．培地追加時に「ひも」を配することで水輸送量が増大した事が収量増加に繋がったものと考えられた．以上の結果より，生育途中で培地量を増やすと同時に，もう1本の「ひも」を配置して大塚A処方1/2濃度で終始管理すれば，生育中期にK欠乏と思しき葉縁の黄変が見られるものの，尻腐れ果実のほとんど発生しない安定したトマト生産が可能であるといえる

Deficiency and Immobility of Magnesium in Cucumber Leaves

キュウリはマグネシウム欠乏症の発生し易い野菜のひとつである.本報告は主として葉におけるマグネシウム含有量の変化並びに移動の難易について検討したものである. 1.培養液の養分除去8日目における成分含有量の減少率は,葉においてP=K>全N>Mg=Ca,根において全N=K>P=Mg>Caとなり,葉のマグネシウムとカルシウム及び根のカルシウムはほとんど変化しなかった. 2.培養液の養分を除去し1か月後に葉分析を行った結果,有果区の葉は摘果区に比べマグネシウムおよびカリの含有率は低く,カルシウムの含有率は高かった.一方,1葉当たりのマグネシウム含有量は有果区に挽いても処理開始時よりわずかに増加し,カリ含有量はわずかに減少した. 3.培養液のマグネシウムを除去し葉に硫酸マグネシウムを散布した結果,散布葉のみが緑色を保ち,その上下節葉はネクロシスを呈した.さらに,散布を葉の一部に限定すると,その部分のみで緑色が保たれた.散布部位におけるマグネシウムの形態別含有率は水溶性アルコール可溶性,および不溶性のいずれも無散布部位より高かったが,とくに水溶性と不溶性マグネシウムにおいて顕著であった. 4.以上の結果,キュウリ葉におけるマグネシウムの移動は容易ではなく,特に,散布したマグネシウムはほとんど移動しないと考えられた

Application of continuous light in a plant factory system 4. Physiological changes and concept of injury induction in plant leaves under continuous light

Physiological changes and concept of injury induction occurring under continuous light are comprehensively reviewed. Continuous light usually reduces photosynthetic rate, which may relate to changes in transpiration and leaf necrosis caused by reactive oxygen species. Other factors apart from photosynthesis may also affect leaf injuries occurring under continuous light. Continuous light sometimes increases carbohydrate and some secondary metabolite contents

Potential for Tomato Cultivation Using Capillary Wick- watering Method

This research was conducted to investigate any potential problems that may be encountered while using capillary wick irrigation system. Medium-fruited tomato plants were cultivated in spring up to the 10th truss. In the first experiment, a 1/5,000a wagner pot was used, and small openings were made 5mm or 50mm from the bottom of the pots. Capillary wicks, 4 cm in width and 45 cm in length were inserted into the slits and aligned to the pot’s inner wall perpendicularly to the soil surface. The other end of the wick was dipped in half or full strength Ohtsuka-A nutrient solution. The wick was covered with water permeable root-barrier material to prevent root penetration into wick. This resulted in good growth and relatively high yield. Without covering, roots grew vigorously into the wicks and 1 month after transplanting extended to the reservoir solution. Removal of root overgrowth caused wilting in some of the plants. This symptom was more pronounced where the wick insertion points were 50 mm from the base. Root contexture was observed in non-covered and imperfectly covered wicks. These results indicate that it is important to make a wick with a perfect covering for stable plant growth and fruit yield. In the second experiment, the capacity of 2 cm or 4 cm wide with and single or double layers of capillary wicks to transport water to the plant root-zone was examined. Some of the plants wilted in the 2 cm single layer, while no differences were observed in other treatments. Furthermore, a high percentage of blossom-end rot was recorded in all treatments. In response to water uptake by the plants, the nutrient solution was supplied daily depending on the decrease of water in the reservoir, in order to maintain the water level fluctuation within a 3 cm range. This fluctuation may account for the blossom-end rot prevalence observed, probably due to water-stress imposed on the plants by unstable water level in the reservoir.「毛管給水ひも」によるトマト栽培の可能性を探るため，中玉トマトの10段摘心栽培を試み，ひもの特性と栽培上の問題点を明らかにした．1/5,000ａワグネルポットの底から5㎜位と50㎜位に小穴を開け，そこへ毛管ひも（幅4㎝，長さ45㎝）の一端を導入し，そのまま鉢中央を横切り対壁に沿って土壌表面まで配置した．他の一端は培養液に浸した．この毛管ひもを遮根透水シートで被覆し（接着あるいは機械織りによるチューブ状管にひもを挿入），その「被覆ひも」を「無被覆ひも」と比較したところ，被覆ひもでは根の侵入が強く抑制でき成育が旺盛で収量も高かった．被覆しないと1か月後には根が毛管ひも内に伸長して貯液槽にまで達した．この根を除去すると多くの個体は萎れの症状を呈した．この症状は50㎜位の方が著しかった．被覆が不完全だと接着部あるいは織り込み部から根は毛管ひもに侵入する．従って，毛管ひもは完全被覆されることが重要であるといえる．また，遮根透水ひもを2㎝幅，4㎝幅としシングル状とダブル状で比較したところ，成育半ばでシングル2㎝幅では萎れ症状が観察され枯死する個体も現れた．その 他では成育等に大きな差異は認められなかったが，いずれの処理区においても多くの尻腐れ果が発生した．トマトの吸水に伴って水位は低下するため本試験では毎日，培養液を手で補給した．しかし，水位変化は最大で3㎝と大きかった．尻腐れ果発生はこの液槽の水位レベル管理に起因するところ大と推察され，今後の課題として残された