Genetic architecture and population structure of Oat Landraces (Avena sativa L.) using molecular and morphological descriptors

439-450Oat is grown as winter forage in India. It is a self-pollinated crop with less variability. However, the variation for different morphological traits in oat germplasm may be available at genotypic level. The present study was conducted to find out the genetic diversity among 24 oat landraces using 9 morphological traits and 24 SSR primers. Morphological data observed across the 24 landraces showed wide variation and grouped various landraces into two clusters. GFY and DMY were positively and significantly correlated with most of the traits studied. The molecular analysis using 24 SSR primers resulted amplification of 62 polymorphic alleles with an average of 2.58 alleles per primer. Size of amplified alleles ranged from 70 to 480 bp. Mean polymorphic information content was 0.42 showing moderate level of SSR polymorphism. Cluster analysis based on SSR data differentiated 24 oat landraces into three major clusters. Bayesian model-based STRUCTURE analysis assigned landraces into two clusters and showed the extent of admixture within individuals. Clustering pattern of oat landraces based on SSR marker profiles were different from that of morphometric traits. So, based on the pooled analysis at morphological and molecular level, the landraces IG-02-121, IG-02-129 and IG-02-113 were found superior for morphological traits as well as most distant among all the landraces under study. Hence, these landraces could be used in for future breeding programmes for genetic improvement in oats

Genetic architecture and population structure of Oat Landraces (Avena sativa L.) using molecular and morphological descriptors

Oat is grown as winter forage in India. It is a self-pollinated crop with less variability. However, the variation fordifferent morphological traits in oat germplasm may be available at genotypic level. The present study was conducted tofind out the genetic diversity among 24 oat landraces using 9 morphological traits and 24 SSR primers. Morphological dataobserved across the 24 landraces showed wide variation and grouped various landraces into two clusters. GFY and DMYwere positively and significantly correlated with most of the traits studied. The molecular analysis using 24 SSR primersresulted amplification of 62 polymorphic alleles with an average of 2.58 alleles per primer. Size of amplified alleles rangedfrom 70 to 480 bp. Mean polymorphic information content was 0.42 showing moderate level of SSR polymorphism. Clusteranalysis based on SSR data differentiated 24 oat landraces into three major clusters. Bayesian model-based STRUCTUREanalysis assigned landraces into two clusters and showed the extent of admixture within individuals. Clustering pattern ofoat landraces based on SSR marker profiles were different from that of morphometric traits. So, based on the pooledanalysis at morphological and molecular level, the landraces IG-02-121, IG-02-129 and IG-02-113 were found superior formorphological traits as well as most distant among all the landraces under study. Hence, these landraces could be used in forfuture breeding programmes for genetic improvement in oats

Genetic diversity amongst oat (Avena sativa) lines for micronutrients and agro-morphological traits

The present experiment was conducted during winter (rabi) seasons of 2019–20 and 2020–21 at the ICAR-Indian Grassland and Fodder Research Institute, Jhansi, Uttar Pradesh to study the genetic diversity amongst oat (Avena sativa L.) lines for micronutrients and agro-morphological traits. For study, 150 oat accessions collected from different sources were evaluated for two years and four micronutrients (Zn, Cu, Fe and Mn) and 9 agro-morphological traits were recorded. Genotypes IG02122 (464.0 mg/kg), IG02156 (48.1 mg/kg), IG03271 (136.0 mg/kg), and IG03213 (22.0 mg/kg) had maximum Fe, Zn, Mn and Cu content in fodder (harvested at 50% flowering). Genotype IG0280 had both high Zn (36.97 mg/kg) and Mn (114.33 mg/kg); IG03233 had high Cu (18.0 mg/kg) and Mn (124.0 mg/kg); and IG02131 had high Cu (18.33 mg/kg) and Fe (369.0 mg/kg) content. Analysis of variance (ANOVA) highlighted significant genotypic differences (P<0.001) for micronutrient content and fodder yield and related traits. High heritability coupled with high genetic advance was found for micronutrients, green fodder yield, test weight, dry matter yield, plant height, tiller number and grain number suggested the preponderance of additive and fixable genetic variance for these traits. The Cu content had significant negative association with Mn content but positive with leaf length and leaf width. Principal component analysis separated the total genetic variation into five main components and covered 59.09% of the total genetic variation. Based on Mahalanobis distances, genotypes were grouped into six clusters where maximum inter-cluster distance was observed for cluster 4 and 5. Therefore, genotypes from these two clusters can be used as parents for the future breeding programmes

Development of Novel Interspecific Fertile Cytotype (4X) Of \u3cem\u3ePennisetum glaucum\u3c/em\u3e X \u3cem\u3ePennisetum purpureum\u3c/em\u3e Utilizing Modified Ploidy Coupled With Embryo Rescue Technique

Interspecific hybrids of genus Pennisetum (P. glaucum x P. purpureum) is the one of the most popular manmade hybrid. It combines the unique features of both P. glaucum (Pearl millet; Bajra) and P. purpureum (Napier; Elephant grass) species, which makes it more resilient to harsh environments with superior fodder quality. Due to ploidy level variation among the parents, these hybrids are sterile and propagated vegetatively only. To overcome this, attempts were made in the present study by exploring the feasibility of novel tetraploid pearl millet (2n=4x=28; Tetra 1; INGR 09047) developed at IGFRI, as a female parent in crossing program involving different Napier genotypes as male parent. Due to limited crossability and hybrid necrosis issues among countless crosses (\u3e 1000), only 1% seed set was initially recorded that too in shriveled state and the developing embryos were aborted after 10-14 days of pollination and fertilization. To save these, embryo rescue technique was standardized and the developing embryos were dissected out aseptically and rescued after 8-10 days of pollination. Continuous crossing programme along with screening of large tissue culture raised nurseries resulted in development of a novel tetraploid seed producing BN hybrid (TBN-20-15) along with 14 novel sterile tetraploid BN hybrids. Presence of univalent chromosomes leads to sterility while proper pairing between parents of TBN-20-15 hybrid have significant effect on fertility. The fertile hybrid is able to produce \u3e15,000-20,000 seeds throughout the year with 80-90% seed germination ability. Their hybridity was confirmed by morphology, molecular and cytogenetic studies. This fertile tetraploid BN hybrid (TBN-20-15) reported for the first time globally will be very helpful in easy and cost-effective dissemination of this highly potential forage crop to the farmer’s field. It has the potential to be the game changer in biofuel production, grassland rejuvenation programs besides bridging the fodder demand supply deficit

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