Violence and alcoholism in the family: how are the children affected?

Alcohol Alcohol. 1998 Jan-Feb;33(1):42-6. Violence and alcoholism in the family: how are the children affected? Malpique C, Barrias P, Morais L, Salgado M, Pinto Da Costa I, Rodriques M. SourceChild and Adolescent Psychiatry Department, Hospital Central de Criancas Maria Pia, Porto, Portugal. Abstract We made an evaluation of how children and adolescents are affected if they live in a family environment where violence associated with alcoholism is a feature. Interviews with 20 families and the use of psychological tests on their children were performed in this study. The study has demonstrated the existence of psychopathological disturbances in those families' children, whose immaturity and insecurity were expressed by aggressive behaviour or by depressive manifestations. It also became evident that there was a transgenerational alcoholism-violence frequency

Handwriting linked to future literacy success: Murdoch Uni researchers find

Handwriting classes may be considered old-fashioned, but new research shows they are key to literacy. Murdoch University School of Education researchers have examined the handwriting abilities of WA pre-primary children in an Australia-first study..

Composição escrita do texto argumentativo: Conhecimento metacognitivo e dificuldades na escrita de alunos do nono ano

Os objetivos deste estudo passaram por avaliar o conhecimento metacognitivo na escrita de estudantes do nono ano (N = 31) em relação aos processos e estratégias de autorregulação que podem ser empregues no processo de composição escrita do texto argumentativo. Por outro lado, pretendeu-se compreender quais as variáveis que, na perspetiva dos alunos, explicam as dificuldades na escrita. A metodologia qualitativa foi a eleita para responder aos objetivos definidos, sendo que os dados obtidos a partir de entrevistas foram submetidos a uma análise de conteúdo com o software N-Vivo. Da análise dos resultados emergem lacunas no conhecimento declarativo, processual e condicional dos participantes. A maioria dos alunos não demostra uma visão integrada das características do texto argumentativo, nem consciência da natureza recursiva e autorregulatória do processo de composição de escrita deste género textual. De salientar ainda que 90% dos alunos atribuem as dificuldades na escrita a variáveis pessoais, valorizando as estratégias comportamentais quando se confrontam com essas dificuldades. Perante estes resultados, discutem-se contributos e implicações para o ensino da escrita e do texto argumentativo em particular, sugerindo-se também potencialidades para o desenvolvimento de intervenções em contexto educativo. NOTE: Article in Portugues

Targeting pancreatic islet PTP1B improves islet graft revascularization and transplant outcomes.

Deficient vascularization is a major driver of early islet graft loss and one of the primary reasons for the failure of islet transplantation as a viable treatment for type 1 diabetes. This study identifies the protein tyrosine phosphatase 1B (PTP1B) as a potential modulator of islet graft revascularization. We demonstrate that grafts of pancreatic islets lacking PTP1B exhibit increased revascularization, which is accompanied by improved graft survival and function, and recovery of normoglycemia and glucose tolerance in diabetic mice transplanted with PTP1B-deficient islets. Mechanistically, we show that the absence of PTP1B leads to activation of hypoxia-inducible factor 1α-independent peroxisome proliferator-activated receptor γ coactivator 1α/estrogen-related receptor α signaling and enhanced expression and production of vascular endothelial growth factor A (VEGF-A) by β cells. These observations were reproduced in human islets. Together, these findings reveal that PTP1B regulates islet VEGF-A production and suggest that this phosphatase could be targeted to improve islet transplantation outcomes

Liver volume and hepatic adiposity in childhood: relations to body growth and visceral fat

The sequence of prenatal growth restraint and postnatal catch-up growth may lead to hepato-visceral adiposity, insulin resistance and low-grade inflammation before the onset of puberty. In prepubertal children born appropriate for gestational age (AGA) or small for gestational age (SGA), we assessed potential relationships between the aforementioned sequence and liver volume.status: publishe

Generation and genetic modification of 3D cultures of human dopaminergic neurons derived from neural progenitor cells

Central nervous system (CNS) disorders remain a formidable challenge for the development of efficient therapies. Cell and gene therapy approaches are promising alternatives that can have a tremendous impact by treating the causes of the disease rather than the symptoms, providing specific targeting and prolonged duration of action. Hampering translation of gene-based therapeutic treatments of neurodegenerative diseases from experimental to clinical gene therapy is the lack of valid and reliable pre-clinical models that can contribute to evaluate feasibility and safety. Herein we describe a robust and reproducible methodology for the generation of 3D in vitro models of the human CNS following a systematic technological approach based on stirred culture systems. We took advantage of human midbrain-derived neural progenitor cells (hmNPCs) capability to differentiate into the various neural phenotypes and of their commitment to the dopaminergic lineage to generate differentiated neurospheres enriched in dopaminergic neurons. Furthermore, we describe a protocol for efficient gene transfer into differentiated neurospheres using CAV-2 viral vectors and stable expression of the transgene for at least 10days. CAV-2 vectors, derived from canine adenovirus type 2, are promising tools to understand and treat neurodegenerative diseases, in particular Parkinson's disease. CAV-2 vectors preferentially transduce neurons and have an impressive level of axonal retrograde transport in vivo. Our model provides a practical and versatile in vitro approach to study the CNS in a 3D cellular context. With the successful differentiation and subsequent genetic modification of neurospheres we are increasing the collection of tools available for neuroscience research and contributing for the implementation and widespread utilization of 3D cellular CNS models. These can be applied to study neurodegenerative diseases such as Parkinson's disease; to study the interaction of viral vectors of therapeutic potential within human neural cell populations, thus enabling the introduction of specific therapeutic genes for treatment of CNS pathologies; to study the fate and effect of delivered therapeutic genes; to study toxicological effects. Furthermore these methodologies may be extended to other sources of human neural stem cells, such as human pluripotent stem cells, including patient-derived induced pluripotent stem cells

Targeting pancreatic islet PTP1B improves islet graft revascularization and transplant outcomes

Deficient vascularization is a major driver of early islet graft loss and one of the primary reasons for the failure of islet transplantation as a viable treatment for type 1 diabetes. This study identifies the protein tyrosine phosphatase 1B (PTP1B) as a potential modulator of islet graft revascularization. We demonstrate that grafts of pancreatic islets lacking PTP1B exhibit increased revascularization, which is accompanied by improved graft survival and function, and recovery of normoglycemia and glucose tolerance in diabetic mice transplanted with PTP1B-deficient islets. Mechanistically, we show that the absence of PTP1B leads to activation of hypoxia-inducible factor 1a -independent peroxisome proliferator-activated receptor y coactivator 1a/estrogen-related receptor a signaling and enhanced expression and production of vascular endothelial growth factor A (VEGF-A) by B cells. These observations were reproduced in human islets. Together, these findings reveal that PTP1B regulates islet VEGF-A production and suggest that this phosphatase could be targeted to improve islet transplantation outcomes