Exigências em energia e proteína líquida para ovelhas da raça Santa Inês em lactação.

Objetivou-se determinar as necessidades de proteína e energia líquida de ovelhas em lactação submetidas a dois manejos nutricionais na gestação e dois tipos de gestação. Os ensaios experimentais foram conduzidos nas instalações do Laboratório de Metabolismo e Calorimetria Animal do Departamento de Zootecnia da Escola de Veterinária da UFMG. Foram utilizadas 28 ovelhas da raça Santa Inês, em um delineamento experimental inteiramente casualizado em arranjo fatorial 2x2, e considerou-se dois tipos de gestação (simples e gemelar) e dois tipos de manejo nutricional (com e sem restrição). As ovelhas eram colocadas sobre plataforma e contidas para realização da ordenha. Para análise dos dados referentes às semanas em lactação utilizou-se o modelo de medidas repetidas no tempo. Os grupos experimentais foram avaliados mediante utilização do teste SNK a 5% de significância. A produção de leite não foi afetada pelo manejo nutricional, bem como a quantidade de gordura, proteína, energia e as necessidades em energia e proteína. Portanto, ficou evidenciado neste estudo que somente o manejo nutricional não foi capaz de alterar as variáveis estudadas. Ovelhas com gestação dupla apresentaram maior produção de leite, maiores teores de gordura e proteína no leite bem como maior valor energético do mesmo. As necessidades em energia e proteína foram maiores para ovelhas com gestação dupla. A produção de leite e as necessidades em energia e proteína foram maiores até a décima semana de lactação. A exigência nutricional em energia e proteína acompanhou a curva de produção de leite. [Net requirement of protein and energy of Santa Inês ewe in lactation]. Abstract: This study aimed to obtain the protein and liquid energy requirements from lactating ewes under two nutritional managements during pregnancy and under two types of pregnancy. Experimental tests were conducted at the Animal Metabolism and Calorimetry Laboratory on Department of Animal Science from Veterinary School of UFMG. For the experimental testing, were used 28 Santa Ines sheeps in a completely randomized design under 2 x 2 factorial arrangement, considering two pregnancy types (single and double) and two nutritional managements (with and without restriction). Sheeps were placed on the platform and contained for milking. To analyse data regarding milking weeks it was used the model of repeated measures in time. The experimental groups were evaluated using the SNK test at 5% significance level. (1) Diets were compound of corn meal (Zea mays), soybean meal (Glycine max), chopped Tifton grass hay (Cynodon spp.) and limestone. Sheep?s specific mineralsupplement was offered ad libitum to the animals Milk production was not affected by nutritional management, as the amount of fat, protein and, energy from milk. The same results were observed to energy and protein requirements. This fact shows that the nutritional management, per se, was not able to change the variables studied. The double pregnancy produced more milk, fat and protein in milk increasing its energy value. Energy and protein requirements were higher in ewes with double pregnancy. Milk production, energy and protein requirements were higher until the tenth week of lactation. Energy and protein nutritional requirement followed the milk production curve

Sugarcane giant borer transcriptome analysis and identification of genes related to digestion.

Sugarcane is a widely cultivated plant that serves primarily as a source of sugar and ethanol. Its annual yield can be significantly reduced by the action of several insect pests including the sugarcane giant borer (Telchin licus licus), a lepidopteran that presents a long life cycle and which efforts to control it using pesticides have been inefficient. Although its economical relevance, only a few DNA sequences are available for this species in the Gen-Bank. Pyrosequencing technology was used to investigate the transcriptome of several developmental stages of the insect. To maximize transcript diversity, a pool of total RNA was extracted from whole body insects and used to construct a normalized cDNA database. Sequencing produced over 650,000 reads, which were de novo assembled to generate a reference library of 23,824 contigs. After quality score and annotation, 43% of the contigs had at least one BLAST hit against the NCBI non-redundant database, and 40% showed similarities with the lepidopteran Bombyx mori. In a further analysis, we conducted a comparison with Manduca sexta midgut sequences to identify transcripts of genes involved in digestion. Of these transcripts, many presented an expansion or depletion in gene number, compared to B. mori genome. From the sugarcane giant borer (SGB) transcriptome, a of aminopeptidase N (APN) cDNAs were characterized based on homology to those reported as Cry toxin receptors. This is the first report that provides a large-scale EST database for the species. Transcriptome analysis will certainly be useful to identify novel developmental genes, to better understand the insect?s biology and to guide the development of new strategies for insect-pest control

Atividade ovicida e larvicida in vitro do óleo essencial de Eucalyptus globulus sobre Haemonchus contortus.

O objetivo deste trabalho foi avaliar o efeito do óleo essencial de Eucalyptus globulus (OEEG) sobre a eclosão e desenvolvimento de larvas de Haemonchus contortus. A determinação da composição química do OEEG foi feita por meio de cromatografia gasosa acoplada à espectrometria de massa. No teste de eclosão de ovos (TEO), utilizaram-se as concentrações 21,75; 17,4; 8,7; 5,43 e 2,71 mg.mL?1; e no de desenvolvimento larvar (TDL) as concentrações foram 43,5; 21,75; 10,87; 5,43 e 2,71 mg.mL?1. Cada ensaio foi acompanhado por um controle positivo, 0,02 mg.mL?1 tiabendazol, no TEO e 0,008 mg.mL?1 ivermectina no TDL, e por um controle negativo com o diluente Tween 80 (3%). A eficácia máxima obtida pelo OEEG sobre ovos foi de 99,3% na concentração de 21,75 mg.mL?1 e, sobre larvas, 98,7% na concentração de 43,5 mg.mL?1. A concentração do OEEG, que inviabilizou 50% dos ovos e das larvas do parasito, foi de 8,3 e 6,92 mg.mL?1, respectivamente. A análise química do óleo identificou como principal componente o monoterpeno 1,8-cineol. O OEEG apresentou atividade ovicida e larvicida in vitro sobre H. contortus, determinando um bom potencial para utilização no controle de nematóides gastrintestinais de ovinos e caprinos

Transcriptome analysis in cotton Boll Weevil (Anthonomus grandis) and RNA interference in insect pests.

Cotton plants are subjected to the attack of several insect pests. In Brazil, the cotton boll weevil, Anthonomus grandis, is the most important cotton pest. The use of insecticidal proteins and gene silencing by interference RNA (RNAi) as techniques for insect control are promising strategies, which has been applied in the last few years. For this insect, there are not much available molecular information on databases. Using 454-pyrosequencing methodology, the transcriptome of all developmental stages of the insect pest, A. grandis, was analyzed. The A. grandis transcriptome analysis resulted in more than 500.000 reads and a data set of high quality 20,841 contigs. After sequence assembly and annotation, around 10,600 contigs had at least one BLAST hit against NCBI non-redundant protein database and 65.7% was similar to Tribolium castaneum sequences. A comparison of A. grandis, Drosophila melanogaster and Bombyx mori protein families’ data showed higher similarity to dipteran than to lepidopteran sequences. Several contigs of genes encoding proteins involved in RNAi mechanism were found. PAZ Domains sequences extracted from the transcriptome showed high similarity and conservation for the most important functional and structural motifs when compared to PAZ Domains from 5 species. Two SID-like contigs were phylogenetically analyzed and grouped with T. castaneum SID-like proteins. No RdRP gene was found. A contig matching chitin synthase 1 was mined from the transcriptome. dsRNA microinjection of a chitin synthase gene to A. grandis female adults resulted in normal oviposition of unviable eggs and malformed alive larvae that were unable to develop in artificial diet. This is the first study that characterizes the transcriptome of the coleopteran, A. grandis. A new and representative transcriptome database for this insect pest is now available. All data support the state of the art of RNAi mechanism in insects

Manejo da broca-gigante da cana-de-açúcar (Telchinlicus) (Drury) (Lepidoptera: Castniidae) no nordeste do Brasil.

bitstream/item/142962/1/Docm-198.pdf; bitstream/item/142121/1/Doc-198.pd

Zoneamento agroecológico das terras do município de Paty do Alferes - Rio de Janeiro, RJ.

O estudo teve o propósito de conhecer a realidade ambiental, de modo integrado, da terras do município

Evaluation of movements of lower limbs in non-professional ballet dancers: hip abduction and flexion

<p>Abstract</p> <p>Background</p> <p>The literature indicated that the majority of professional ballet dancers present static and active dynamic range of motion difference between left and right lower limbs, however, no previous study focused this difference in non-professional ballet dancers. In this study we aimed to evaluate active movements of the hip in non-professional classical dancers.</p> <p>Methods</p> <p>We evaluated 10 non professional ballet dancers (16-23 years old). We measured the active range of motion and flexibility through Well Banks. We compared active range of motion between left and right sides (hip flexion and abduction) and performed correlation between active movements and flexibility.</p> <p>Results</p> <p>There was a small difference between the right and left sides of the hip in relation to the movements of flexion and abduction, which suggest the dominant side of the subjects, however, there was no statistical significance. Bank of Wells test revealed statistical difference only between the 1^stand the 3^rdmeasurement. There was no correlation between the movements of the hip (abduction and flexion, right and left sides) with the three test measurements of the bank of Wells.</p> <p>Conclusion</p> <p>There is no imbalance between the sides of the hip with respect to active abduction and flexion movements in non-professional ballet dancers.</p