34 research outputs found
Imaging the boundaries—innovative tools for microscopy of living cells and real-time imaging
Recently, light microscopy moved back into the spotlight, which is mainly due to the development of revolutionary technologies for imaging real-time events in living cells. It is truly fascinating to see enzymes “at work” and optically acquired images certainly help us to understand biological processes better than any abstract measurements. This review aims to point out elegant examples of recent cell-biological imaging applications that have been developed with a chemical approach. The discussed technologies include nanoscale fluorescence microscopy, imaging of model membranes, automated high-throughput microscopy control and analysis, and fluorescent probes with a special focus on visualizing enzyme activity, free radicals, and protein–protein interaction designed for use in living cells
Limiting prolonged inflammation during proliferation and remodeling phases of wound healing in streptozotocin-induced diabetic rats supplemented with camel undenatured whey protein
Modulação androgênica e estrogênica na próstata: uma abordagem em modelos experimentais de roedores com enfoque na biologia estrutural
Wide-field subdiffraction RESOLFT microscopy using fluorescent protein photoswitching.
Subdiffraction fluorescence imaging is presented in a parallelized wide-field arrangement exploiting the principle of reversible saturable/switchable optical transitions (RESOLFT). The diffraction barrier is overcome by photoswitching ensembles of the label protein asFP595 between a nonfluorescent off- and a fluorescent on-state. Relying on ultralow continuous-wave intensities, reversible protein switching facilitates parallelized fast image acquisition. The RESOLFT principle is implemented by illuminating with intensity distributions featuring zero intensity lines that are further apart than the conventional Abbe resolution limit. The subdiffraction resolution is verified by recording live Escherichia coli bacteria labeled with asFP595. The obtained resolution of 50 nm ( approximately lambda/12) is limited only by the spectroscopic properties of the proteins and the imperfections of the optical implementation, but not on principle grounds