4 research outputs found
High intensity focused ultrasound for the treatment of localized prostate cancer: efficacy and impact on sexual function
Experimental pancreas transplantation: the consequences of the portocaval shunt on the blood glucose, plasma insulin, and glucagon Transplante experimental de pâncreas: as consequĂŞncias da derivação portocava na glicemia sanguĂnea, na insulina plásmica e no glucagon
The consequences of the bypass of the portal venous effluent into the systemic circulation on the blood glucose, plasma insulin, and glucagon in immediate postoperative period of rats submitted to pancreas transplantation were studied. Forty outbred male Wistar rats were randomly assigned to two experimental groups: group NC included 20 non-diabetic control rats, submitted to simulated operating (sham-operated), and group PT included 20 diabetic rats that received heterotopic pancreas transplantation from normal donor Wistar rats. For 7 days prior and 1, 3, 6, 12, 24, 48, 72 and 96 hours after transplantation blood glucose, plasma insulin, and glucagon were recorded. These parameters were also concurrently recorded for NC rats. Diabetes was induced by i.v. alloxan administration; PT rats were immunosuppressed with cyclosporin A. NC rats presented normal values of blood glucose, plasma insulin, and glucagon over the course of experiment. A marked hyperinsulinemia was found in peripheral venous blood of PT rats, being plasma insulin significantly higher than that for NC rats (P<0.01) beginning 72- h after transplant. The plasma glucagon, elevated in pre-transplant period, did not change after transplant. Despite hyperinsulinemia and hyperglucagonemia, the blood glucose levels were elevated up to 6- h after transplant, but were within normal levels following this period. Beginning at 12- h after transplant the blood glucose levels observed in PT rats did not differ significantly to NC rats until the sacrifice.<br>A proposição foi realizada, no perĂodo pĂłs-operatĂłrio imediato, em 40 ratos Wistar, distribuĂdos por sorteio em doi grupos: grupo NC, vinte ratos correspondentes ao grupo controle, nĂŁo diabĂ©tico, submetidos a operação simulada e o grupo PT, 20 ratos correspondentes ao grupo diabĂ©tico que recebeu transplante de pâncreas heterotĂłpico de ratos Wistar normais. Durante sete dias, antes do transplante, e 1, 3, 6, 12, 24, 48, 72, 96 horas apĂłs, determinava-se a glicose sanguĂnea, a insulina plasnática e o glucagon. Estes parâmetros eram obtidos tambĂ©m do grupo NC. Diabetes mellitus experimental era induzida pela administração intravenosa de aloxana. O grupo PT era imunosuprimido com ciclosporina A. O grupo NC apresentou nĂveis normais de glicose sanguĂnea, de insulina plásmica e de glucagon, durante todo o experimento. Foi encontrada nĂtida hiperinsulinemia no sangue venoso perifĂ©rico do grupo PT. A insulina plasmática era significantemente maior no grupo PT comparada ao grupo NC começando 72 horas apĂłs o transplante. O glucagon plasmático, elevado no perĂodo prĂ©-transplante, nĂŁo se alterou apĂłs o transplante. Apesar de hiperinsulinemia e hiperglucagonemia, os nĂveis de glicose sanguĂnea eram elevados 6 horas apĂłs o transplante e mantiveram-se normais apĂłs este perĂodo. Considerando-se os nĂveis de glicose sanguĂnea 12 horas pĂłs-transplante, nĂŁo houve diferença estatisticamente significante entre os grupos PT e NC, atĂ© o sacrifĂcio