ALVEOLAR RIDGE AUGMENTATION WITH TITANIUM MESH AND A COMBINATION OF AUTOGENOUS BONE AND ANORGANIC BOVINE BONE: A 2-YEAR PROSPECTIVE STUDY

Background: Recently, the use of bovine bone mineral (BBM) in combination with autogenous bone for alveolar ridge augmentation before implant placement has increased in favor because of concerns over morbidity associated with extraoral donor sites. The aim of this prospective study was to evaluate the clinical and radiographic parameters of implants placed in augmented ridges using a 70:30 mixture of autogenous bone and BBM in association with micro-mesh over a 2-year period. Methods: Sixteen partially edentulous patients requiring bone augmentation were consecutively treated for 19 reconstructive procedures and delayed implant placement (44 implants) after 8 to 9 months of submerged mesh healing. Clinical examinations were performed and radiographs of the implants were taken 6 months after prosthetic loading and once a year during a 2-year follow-up. Results: Only one (5.3%) of the 19 micro-meshes became exposed after 2 months and was removed. Computed tomography scans of the alveolar ridge pre- and postreconstruction demonstrated mean vertical augmentation of 3.71 +/- 1.24 mm and mean horizontal augmentation of 4.16 +/- 0.59 mm. All of the implants were retained after 2 years, yielding a 100% survival rate. The mean bone resorption around the implants was 1.37 +/- 0.32 mm during the observation period. Only three implants demonstrated bone resorption >2 mm, whereas 41 implants were considered clinically successful, resulting in a success rate of 93.1%. Conclusion: This 2-year prospective study demonstrated that implants placed into augmented bone using this technique exhibited peri-implant stability with high survival (100%) and success (93.1%) rates

Development and evaluation of a decellularized membrane from human dermis

Interest is increasing in biological scaffolds for tissue regeneration, such as extracellular matrix (ECM) membranes, developed through soft tissue decellularization. The present study describes the development of a chemicophysical decellularization method applied to allogenic human-derived dermis (HDM). To evaluate the absence of viable cells and the maintenance of ECM structure, biological, histological and ultrastructural assessments were performed on the HDM membrane. Residual DNA content and glycosaminoglycan (GAG) and collagen contents were quantified. Growth factor (GF) release was directly measured on HDM extracts and indirectly measured by assessing cell proliferation after administering extract to cultures. Tensile tests were performed to measure the effect of the decellularization technique on the mechanical properties of tissue. Histocompatibility was investigated after subcutaneous implantation in rats. Residual DNA, GAG and collagen content measurements, vitality index, histology and electron microscopy showed the efficiency of the decellularization process and preservation of ECM matrix and bioactivity. In HDM extracts, among the tested GFs, transforming growth factor-\u3b21 showed the highest concentration. HDM extracts significantly increased the proliferation rate of L929 fibroblasts in comparison with controls (p\u2009<\u20090.005, p\u2009<\u20090.05 and p\u2009<\u20090.0005). Maximum load and stiffness of HDM were significantly higher than those of cellularized dermis (p\u2009<\u20090.0005, p\u2009<\u20090.005). Histological and histomorphometric analysis of explanted samples showed that the membrane was integrated with host tissues in the absence of inflammatory reactions. Our results show that the decellularization method allowed the development of a human allograft dermal matrix that might be useful for soft tissue regeneration

Destination of titanium particles detached from titanium plasma sprayed endosseous dental implants

none8noneE.Orsini; M.Franchi; D.Martini; B.Bacchelli; A.Trirè; M.Quaranta; M.Fini; A.RuggeriE.Orsini; M.Franchi; D.Martini; B.Bacchelli; A.Trirè; M.Quaranta; M.Fini; A.Rugger