Transient activaton of β-catenin signalling in adult mouse epidermis is sufficient to induce new hair follicles but continuous activation is required to maintain hair follicle tumours

When β-catenin signalling is disturbed from mid-gestation onwards lineage commitment is profoundly altered in postnatal mouse epidermis. We have investigated whether adult epidermis has the capacity for β-catenin-induced lineage conversion without prior embryonic priming. We fused N-terminally truncated, stabilised β-catenin to the ligand-binding domain of a mutant oestrogen receptor (ΔNβ -cateninER). ΔNβ-cateninER was expressed in the epidermis of transgenic mice under the control of the keratin 14 promoter and β -catenin activity was induced in adult epidermis by topical application of 4-hydroxytamoxifen (4OHT). Within 7 days of daily 4OHT treatment resting hair follicles were recruited into the hair growth cycle and epithelial outgrowths formed from existing hair follicles and from interfollicular epidermis. The outgrowths expressed Sonic hedgehog, Patched and markers of hair follicle differentiation, indicative of de novo follicle formation. The interfollicular epidermal differentiation program was largely unaffected but after an initial wave of sebaceous gland duplication sebocyte differentiation was inhibited. A single application of 4OHT was as effective as repeated doses in inducing new follicles and growth of existing follicles. Treatment of epidermis with 4OHT for 21 days resulted in conversion of hair follicles to benign tumours resembling trichofolliculomas. The tumours were dependent on continuous activation of β-catenin and by 28 days after removal of the drug they had largely regressed. We conclude that interfollicular epidermis and sebaceous glands retain the ability to be reprogrammed in adult life and that continuous β-catenin signalling is required to maintain hair follicle tumours.Peer reviewedFinal Published versio

Mitochondrial heteroplasmy in an avian hybrid form (Passer italiae: Aves, Passeriformes)

Mitochondrial heteroplasmy is the result from biparental transmission of mitochondrial DNA (mtDNA) to the offspring. In such rare cases, maternal and paternal mtDNA is present in the same individual. Though recent studies suggested that mtDNA heteroplasmy might be more common than previously anticipated, that phenomenon is still poorly documented and was mostly detected in case studies on hybrid populations. The Italian sparrow, Passer italiae is a homoploid hybrid form that occurs all across the Italian Peninsula mostly under strict absence of either of its parent species, the house sparrow (P. domesticus) and the Spanish sparrow (P. hispaniolensis). In this study, we document a new case of mitochondrial heteroplasmy from two island populations of P. italiae (Ustica and Lipari). Our analysis was based on the mitochondrial NADH dehydrogenase subunit 2 (ND2) that allows for a clear distinction between mitochondrial lineages of the two parental species. We amplified and sequenced the mitochondrial ND2 gene with specifically designed primer combinations for each of the two parental species. In two of our study populations, a single individual carried two different ND2 haplotypes from each of the two parental lineages. These findings contribute to current knowledge on the still poorly documented phenomenon of paternal leakage in vertebrates

Simple Non-Markovian Microscopic Models for the Depolarizing Channel of a Single Qubit

The archetypal one-qubit noisy channels ---depolarizing, phase-damping and amplitude-damping channels--- describe both Markovian and non-Markovian evolution. Simple microscopic models for the depolarizing channel, both classical and quantum, are considered. Microscopic models which describe phase damping and amplitude damping channels are briefly reviewed.Comment: 13 pages, 2 figures. Title corrected. Paper rewritten. Added references. Some typos and errors corrected. Author adde

Beating the PNS attack in practical quantum cryptography

In practical quantum key distribution, weak coherent state is often used and the channel transmittance can be very small therefore the protocol could be totally insecure under the photon-number-splitting attack. We propose an efficient method to verify the upper bound of the fraction of counts caused by multi-photon pluses transmitted from Alice to Bob, given whatever type of Eve's action. The protocol simply uses two coherent states for the signal pulses and vacuum for decoy pulse. Our verified upper bound is sufficiently tight for QKD with very lossy channel, in both asymptotic case and non-asymptotic case. The coherent states with mean photon number from 0.2 to 0.5 can be used in practical quantum cryptography. We show that so far our protocol is the $only$ decoy-state protocol that really works for currently existing set-ups.Comment: So far this is the unique decoy-state protocol which really works efficiently in practice. Prior art results are commented in both main context and the Appendi