Deutsch-Japanische Kooperation: Entwicklung von schnellen multidimensionalen optischen Immunsensoren fuer den Nachweis von Wasserverunreinigungen Abschlussbericht

In a german-japanese co-operation an immunochemical sensor with multianalyte ability for water analysis has been developed. This sensor represents a new approach to solve the problem of immunological cross-reactivities of structurally related compounds. The principle of this new sensor system is based on the parallelization of individual immunological assays. A sample may be analyzed towards many different contaminants in parallel with this sensor. Two competitive assay formats can be applied for this purpose: In a direct assay format small spots of different antibodies are immobilized on a glass slide in an array structure. In an indirect assay format haptens are immobilized on the glass side, which are complementary to the antibodies. (orig.)Available from TIB Hannover: F99B763 / FIZ - Fachinformationszzentrum Karlsruhe / TIB - Technische InformationsbibliothekSIGLEBundesministerium fuer Bildung und Forschung (BMBF), Bonn (Germany)DEGerman

Labeling strategies for bioassays \ud

Different labeling strategies for enzymatic assays and immunoassays are reviewed. Techniques which make use of direct detection of a label, e.g. radioimmunoassays, are discussed, as are techniques in which the label is associated with inherent signal amplification. Examples of the latter, e.g. enzyme-linked immunosorbent assays or nanoparticle-label based assays, are presented. Coupling of the bioassays to chromatographic separations adds selectivity but renders the assays more difficult to apply. The advantages and drawbacks of the different analytical principles, including future perspectives, are discussed and compared. Selected applications from clinical, pharmaceutical, and environmental analysis are provided as examples