6 research outputs found
Dualism of mixed chimerism between hematopoiesis and stroma in chronic idiopathic myelofibrosis after allogeneic stem cell transplantation
Scant knowledge exists concerning lineagerestricted
mixed chimerism (mCh) after allogeneic
peripheral blood stem cell transplantation (PSCT) in
patients with chronic idiopathic myelofibrosis (CIMF).
Following a sex-mismatched PSCT, a combined
immunopheno- and genotyping by fluorescence in-situ
hybridization (FISH) was performed on sequential bone
marrow (BM) biopsies at standardized intervals. Results
were compared with PCR analysis of corresponding
peripheral blood samples in five patients. According to
FISH, pretransplant specimens revealed a gender
congruence of more than 99%, while in the first three
months the total BM exhibited a persistent fraction of
host cells (30% to 40%) with a tendency to decline after
about one year. It is noteworthy that the majority of
endothelial cells maintained a recipient origin, whereas
CD34+ progenitors and especially CD61+ megakaryocytes
exhibited only very few host-derived cells. In
keeping with the prevalence of donor cells in the
hematopoietic compartment, PCR analysis of peripheral
blood cells displayed a non-significant degree of mCh.
In conclusion, according to FISH and PCR analysis,
successful PSCT in CIMF results in an almost complete
chimeric (donor-derived) state of the hematopoietic cell
population. The non-transplantable stromal compartment
includes the vascular endothelium with a predominance
of recipient cells. The minimal mCh of this population
implies probably a donor-derived origin (endothelial
progenitor cells)