Diversity of <i>mariner</i>-like elements in Orthoptera

Mariner-like elements (MLEs) are among the most widespread DNA transposable elements in eukaryotes. Insects were the first organisms in which MLEs were identified, however the diversity of MLEs in the insect order Orthoptera has not yet been addressed. In the present study, we explore the diversity of MLEs elements in 16 species of Orthoptera belonging to three infraorders, Acridoidea (Caelifera), Grylloidea (Ensifera), and Tettigoniidea (Ensifera) by combining data mined from computational analysis of sequenced degenerative PCR MLE amplicons and available Orthoptera genomic scaffolds. In total, 75 MLE lineages (Ortmar) were identified in all the studied genomes. Automatic phylogeny-based classification suggested that the current known variability of MLEs can be assigned to seven statistically well-supported phylogenetic clusters (I–VII), and the identified Orthoptera lineages were distributed among all of them. The majority of the lineages (36 out of 75) belong to cluster I; 20 belong to cluster VI; and seven, six, four, one and one lineages belong to clusters II, IV, VII, III, and V, respectively. Two of the clusters (II and IV) were composed of a single Orthoptera MLE lineage each (Ortmar37 and Ortmar45, respectively) which were distributed in the vast majority of the studied Orthoptera genomes. Finally, for 16 Orthoptera MLE lineages, horizontal transfer from the distantly related taxa belonging to other insect orders may have occurred. We believe that our study can serve as a basis for future researches on the diversity, distribution, and evolution of MLEs in species of other taxa that are still lacking the sequenced genomes

THE CURRENT STATE OF DATA TRANSMISSION CHANNELS FROM PUSHCHINO TO MOSCOW AND PERSPECTIVES

Since the work of a unique space radio telescope in the international VLBI project ”Radioastron” extended to 2017 the transmission and storage of large volumes of scientific and telemetry data obtained during the experiments is still remains actual. This project is carried out by theAstroSpaceCenter of Lebedev Physical Institute inMoscow,Russia. It requires us to maintain in operating state the high-speed link to merge into a single LAN buffer data center in Puschino and scientific information center inMoscow. Still relevant the chanal equipment monitoring system, and storage systems, as well as the timely replacement of hardware and software upgrades, backups, and documentation of the network infrastructure.

ACUTE CEREBROVASCULAR EVENTS IN PATIENTS WITH ARTERIAL HYPERTENSION: MOLECULAR GENETIC ASPECTS

Aim. To study association between stroke and gene polymorphism of angeotensin converting enzyme (ACE), angeotensin II type 1 receptor (ATR1), apolipoprotein СIII (APO CIII), apoproteine Е (APO E), methylentetrahydrofolate reductase (MTHFR), fibrinogen (Fb), endothelial NO-synthase (NOS3) in arterial hypertension (HT).Material and methods. Molecular genetic analysis by polymerase chain reaction was done in 41 patients with HT, who experienced first episode of acute disturbances of cerebral blood circulation (ADCBC).Results. Stroke rate in patients with HT is associated with A1166C of ATR1 gene polymorphism, G-455A of Fb gene polymorphism and C677T of MTHFR gene polymorphism. The high risk markers are C-allele of ATR1 gene, -455А allele and AA genotype of Fb gene, 677T allele of MTHFR. The A-allele and genotype AA of ATR1 gene, G-445 allele of Fb gene, С677-allele and CC genotype of MTHFR gene play protective role against ADCBC in HT.Conclusion. It is established an association between gene polymorphism of some molecules and ADCBC in HT

DATA NETWORKING SUPPORT SERVICES OF PUSHCHINO RADIOASTRONOMY OBSERVATORY, ASTROSPACE CENTER OF LEBEDEV PHYSICAL INSTITUTE

Growth of local area network Observatory associated with employee needs to access network resources has led to an increase in the number of computing and network devices. Until recently, we tested performance of these systems excellent manual and most of the problems and faults detected already on the fact the accident occurred. For a small number of network nodes, manual monitoring is not a significant problem, but with increasing the number of nodes troubles hooting becomes a very difficult task for network and system administrators and outage in work of network services Observatory may becomes critical. There fore there was a need to automate the monitoring service network resources and servers. Growth of local area network Observatory associated with employee needs to access network resources has led to an increase in the number of computing and network devices. Until recently, we tested performance of these systems excellent manual and most of the problems and faults detected already on the fact the accident occurred. For a small number of network nodes, manual monitoring is not a significant problem, but with increasing the number of nodes troubles hooting becomes a very difficult task for network and system administrators and outage in work of network services Observatory may becomes critical. There fore there was a need to automate the monitoring service network resources and servers

INFORMATION TELECOMMUNICATIONS OF PUSHCHINO RADIO ASTRONOMY OBSERVATORY, ASTRO SPACE CENTER OF LEBEDEV PHYSICAL INSTITUTE

Buffer data center was created in the territory of the Pushchino Radio Astronomy Observatory three years ago. The necessity of its creation was caused by the high requirements to the speed and quality of the transmission large amounts of scientific and telemetry data received by tracking station RT-22 from the space radio telescope of the international project “Radioastron”. The transfer of this data is carried out over a long distance over100 kmfrom the Pushchino toMoscowcenter of processing and storage ASC FIAN. And now we use the data center as a center of local network of the Observatory.

RESULTS OF TREATMENT OF OPEN FRACTURES IN PATIENTS WITH POLYTRAUMA IN A REGIONAL TRAUMA SYSTEM

Aim. To analyze options for treatment tactics for open fractures of bones, implemented in a regional trauma system in theKrasnodar Territory.Materials and methods. Retrospective analysis includes the results of treatment of 82 patients with polytrauma and severe open fractures of the shin bones aged from 18 to 60 years, of whom 23 in unstable or critical condition, hospitalized in 2016-2017 in the Scientific Research Institution – S.V. Ochapovsky Regional Clinical Hospital № 1.Results. Treatment tactics for patients with severe polytrauma and open fractures type IIIA and B according to the classification of Gustilo-Andersen adopted in trauma center of level I, reduces the total number of infectious complications to 9,09% for fractures of type III A and 53,84% of IIIB fractures compared to option treatment strategies adopted in trauma centres II, III where the level of infection reaches 58,06% fractures type III A and 93,75% of IIIB fractures. The typical mistakes made in primary surgical treatment in patients with polytrauma and severe open fractures were revealed: incomplete revision of the wound with leaving foreign bodies, non-viable soft tissues, unstable fixation of the fracture with the help of a skeletal traction system, incomplete external fixation of the fracture with a device or plaster bandage, suturing of the wound with relaxing skin incisions during soft tissue swelling, absence of drains or passive drainage of the open fracture.Conclusion. The usage of primary surgical treatment in a reduced volume, with the application of VAC-dressings, in patients with polytrauma and severe open fractures of the limb bones can reduce the overall incidence of infectious complications