Minimal deformations of the commutative algebra and the linear group GL(n)

We consider the relations of generalized commutativity in the algebra of formal series $M_q (x^i )$, which conserve a tensor $I_q$-grading and depend on parameters $q(i,k)$ . We choose the $I_q$-preserving version of differential calculus on $M_q$ . A new construction of the symmetrized tensor product for $M_q$-type algebras and the corresponding definition of minimally deformed linear group $QGL(n)$ and Lie algebra $qgl(n)$ are proposed. We study the connection of $QGL(n)$ and $qgl(n)$ with the special matrix algebra \mbox{Mat} (n,Q) containing matrices with noncommutative elements. A definition of the deformed determinant in the algebra \mbox{Mat} (n,Q) is given. The exponential parametrization in the algebra \mbox{Mat} (n,Q) is considered on the basis of Campbell-Hausdorf formula.Comment: 14 page

Молекулярно-генетическое исследование стабильности и подтверждение подлинности штамма Внуково-32, применяемого для производства вакцины антирабической культуральной концентрированной очищенной инактивированной сухой

Rabies is an acute viral disease caused by a virus of the Rhabdoviridae family of the Lyssavirus genus, which affects the central nervous system and is characterised by absolute mortality. Vaccination is the only way to prevent the disease in humans. One of the products used for vaccination is a cultural concentrated purified inactivated dry rabies vaccine produced by the Federal State Budgetary Institution of Science “Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences” (hereinafter—Chumakov Center).The aim of the study was to examine the structure of the working virus seed of Vnukovo-32 strain used by the Chumakov Center for rabies vaccine production, to assess its genetic stability during production, to explore the possibility of using molecular genetic methods for identification of the production strain in the finished dosage form, and to study the nucleotide sequence of the CVS strain.Materials and methods: Vnukovo-32 rabies virus production strain, working virus seeds, finished batches of the rabies vaccine, CVS fixed rabies virus strain used in the assessment of specific immunity. The molecular genetic study was performed using RT-PCR followed by restriction and sequencing.Results: the paper presents the results of nucleotide sequence analysis of the G gene fragment obtained from the Vnukovo-32 production strain, batches of the working virus seed, and finished batches of the rabies vaccine produced in 2012, 2018, and 2019, and the CVS fixed rabies virus strain used in the assessment of the vaccine’s specific immunity. The study demonstrated that restriction analysis could be used for Vnukovo-32 strain identification at all production stages, including the finished dosage form.Conclusion: Vnukovo-32 and CVS strains used by the Chumakov Center are rabies viruses. Analysis of the nucleotide sequence of the G gene fragment showed that the Vnukovo-32 strain remains stable throughout different production stages. The obtained nucleotide sequence of gene G of the Vnukovo-32 strain was deposited in GenBank (accession number MN116503). The study demonstrated that restriction analysis could be used for Vnukovo-32 strain identification at all production stages, including the finished dosage form. Бешенство – острая вирусная инфекция, вызываемая вирусом семейства Rhabdoviridae рода Lyssavirus и характеризующаяся симптомами поражения центральной нервной системы и абсолютной летальностью. Единственной возможностью предотвратить возникновение данного заболевания у людей является вакцинопрофилактика. Одним из препаратов, используемых в этих целях, является вакцина антирабическая культуральная концентрированная очищенная инактивированная сухая, выпускаемая ФГБНУ «ФНЦИРИП им. М. П. Чумакова РАН».Цель работы: исследование структуры производственного, рабочего посевного вируса бешенства штамма Внуково-32, используемого ФГБНУ «ФНЦИРИП им. М. П. Чумакова РАН» для производства антирабической вакцины, его генетической стабильности на этапах производства, изучение возможности применения молекулярно-генетических методов для подтверждения подлинности производственного штамма в готовой форме вакцины и изучение нуклеотидной последовательности штамма CVS.Материалы и методы: производственный штамм вируса бешенства Внуково-32, рабочие посевные вирусы, готовые серии вакцины антирабической, штамм CVS фиксированного вируса бешенства, используемый для оценки специфического иммунитета. Молекулярно-генетическое исследование проведено с использованием ОТ-ПЦР с последующей рестрикцией и секвенированием.Результаты: представлены результаты анализа нуклеотидных последовательностей фрагмента гена G, полученного из производственного штамма Внуково-32, серий рабочего посевного вируса и готовых серий вакцины антирабической, изготовленных в 2012, 2018, 2019 г., штамма фиксированного вируса бешенства CVS, используемого для оценки специфической активности вакцины. Показана возможность применения рестрикционного анализа для подтверждения подлинности штамма Внуково-32 на всех этапах производства, включая готовую форму вакцины.Заключение: штаммы Внуково-32 и CVS, используемые в ФГБНУ «ФНЦИРИП им. М. П. Чумакова РАН», являются вирусами бешенства. Анализ нуклеотидной последовательности фрагмента гена G показал, что штамм Внуково-32 стабилен на разных этапах производства. Полученная нуклеотидная последовательность гена G штамма Внуково-32 депонирована в GenBank (номер MN116503). Показана возможность применения рестрикционного анализа для подтверждения подлинности штамма Внуково-32 вируса бешенства на всех этапах производства, включая готовую форму вакцины

Adaptation features of sheep of the Edilbaev breed reared in the agroecological conditions of the arid zones of Southern Russia

Aim. The aim of the research was to study the features of formation of adaptive  ability, meat productivity and quality indicators of mutton obtained in the arid conditions of the Volga region from sheep of the Edilbaev breed of different genotypes. Material and Methods. A package of teaching materials has been developed concerning an increase in the productive qualities of different genotypes of the studied  breed in the conditions of arid regions of southern Russia.   Results. The authors’ research has demonstrated the high adaptive abilities of animals of the Edilbaev breed, their economic and biological qualities, food and taste  indicators of their meat, as well as the possibility of further development of the  breed in the arid conditions of southern Russia. As a research base, we selected the  Volgograd‐Edilbay LLC breeding farm (the world's only breeding and genetic center  for raising Edilbaev sheep) which is located in the Volgograd region and specializes  in breeding pure‐bred animals in arid steppe, semi‐desert and desert zones. Due to  the great demand for the livestock of this breed from farms located in arid territories, this study of the comparative aspects of production characteristics of animals  of original and new types in the arid conditions of the Volga region is of interest to  both science and practical animal husbandry.   Conclusion. An assessment of the nutritional value of mutton obtained from animals of the Edilbaev breed has shown its high nutritional and biological qualities

Isolation of a human lactoferrin biosimilar from frozen milk of goat producers

This study offers an additional source of information on isolation and purification of the human lactoferrin biosimilar from frozen milk of goat producers. Thanks to the application of an improved technique of raw material degreasing, a milk filtrate with complete absence of the fat part was obtained, which as a result had a favorable effect on the yield and properties of the finished product, whose quality was confirmed by methods of MALDI-TOF mass spectrometry and microbiological analysis. As a result 135 g of human lactoferrin biosimilar was obtained from 75 liters of frozen milk

Molecular Genetic Testing of Stability and Identification of Vnukovo-32 Strain Used for Production of the Cultural Concentrated Purified Inactivated Dry Rabies Vaccine

Rabies is an acute viral disease caused by a virus of the Rhabdoviridae family of the Lyssavirus genus, which affects the central nervous system and is characterised by absolute mortality. Vaccination is the only way to prevent the disease in humans. One of the products used for vaccination is a cultural concentrated purified inactivated dry rabies vaccine produced by the Federal State Budgetary Institution of Science “Chumakov Federal Scientific Center for Research and Development of Immune-and-Biological Products of Russian Academy of Sciences” (hereinafter—Chumakov Center).The aim of the study was to examine the structure of the working virus seed of Vnukovo-32 strain used by the Chumakov Center for rabies vaccine production, to assess its genetic stability during production, to explore the possibility of using molecular genetic methods for identification of the production strain in the finished dosage form, and to study the nucleotide sequence of the CVS strain.Materials and methods: Vnukovo-32 rabies virus production strain, working virus seeds, finished batches of the rabies vaccine, CVS fixed rabies virus strain used in the assessment of specific immunity. The molecular genetic study was performed using RT-PCR followed by restriction and sequencing.Results: the paper presents the results of nucleotide sequence analysis of the G gene fragment obtained from the Vnukovo-32 production strain, batches of the working virus seed, and finished batches of the rabies vaccine produced in 2012, 2018, and 2019, and the CVS fixed rabies virus strain used in the assessment of the vaccine’s specific immunity. The study demonstrated that restriction analysis could be used for Vnukovo-32 strain identification at all production stages, including the finished dosage form.Conclusion: Vnukovo-32 and CVS strains used by the Chumakov Center are rabies viruses. Analysis of the nucleotide sequence of the G gene fragment showed that the Vnukovo-32 strain remains stable throughout different production stages. The obtained nucleotide sequence of gene G of the Vnukovo-32 strain was deposited in GenBank (accession number MN116503). The study demonstrated that restriction analysis could be used for Vnukovo-32 strain identification at all production stages, including the finished dosage form