Scanning Electron Microscopic Study of the Surface of Feline Gastric Epithelium: A Simple Method of Removing the Coating Material

Scanning electron microscopic examination of the gastric surface epithelial cells is often hindered by the presence of a coating material. Several methods for removal of coating material on feline gastric mucosa were utilized. The cleansed tissues were evaluated using the scanning electron microscope to assess damage caused by the use of various cleansing methods to surface epithelial cells. The stretched stomach washed several times, including rubbing the mucosal surface with gloved fingers, yielded the best results with no apparent damage to the surface epithelial cells. Flushing unstretched stomachs with saline only did not adequately remove coating material. Flushing unstretched stomachs with saline while stroking the surface with a cotton tipped applicator stick removed debris but damaged the surface epithelium

The Normal Structure of Regional Feline Gastric Mucosae: Scanning Electron Microscopic Study

Regions of cat\u27s stomach can be identified by looking at the surface epithelial cells by scanning electron microscopy (SEM). The luminal surface of cells of the cardiac region were elongated, of the fundus rounded, of the corpus polygonal shaped, and of the pyloric region diamond shaped. The quantity and distribution of microvilli covering the epithelial cells varies, being abundant and evenly distributed in the cardiac region and gradually decreasing in number toward the gastro-duodenal junction, where they were confined to cell perimeters. The colliculi varied in shape and distribution from few in the fundus and corpus to numerous in the pyloric region. Large numbers of gastric pits were present in the corpus. They diminish toward both the cardia and gastro-duodenal junction. The cardiac and pyloric glands were coiled. The gastric glands (glandula gastrica propria) were straight tubules in the fundus and coiled in the corpus. All luminal surfaces of glandular epithelial cells were covered with microvilli, but the regional distribution of microvilli on the cell was variable. Parietal, mucous neck, and chief (zymogen) cells were identified by their cytoplasmic structure. Parietal cells had long apical microvilli, mucous neck cells contained large numbers of globular mucous granules, and chief cells were vacuolated. A few G cells (Endocrinocytus gastrointestinalis) were seen in the cardiac region, large numbers in the pyloric region, and not found in fundus or corpus

A histological study of the effect of saline and povidone-iodine infusions on the equine endometrium

A study was conducted to assess the reaction of the endometrium of the mare to both saline and povidone-iodine infusions. In the control group (Group 1), uterine biopsies were taken at 0, 3, 5, 7, 10, 15, 20 and 30 days from the beginning of the experimental period. The treatment groups had intrauterine infusions of saline (Group 2) or 1% povidone iodine in saline (Group 3) on Days 0 and 2, and had endometrial biopsies taken on the same days as the control group. The concentration of inflammatory cells in the endometria of the Group-2 mares paralleled that of the Group-1 mares but was at a slightly higher level. Group-3 mares demonstrated significant increases in the numbers of inflammatory cells. An acute reaction was observed in Group-3 mares until Days 7 to 10. Thereafter, the inflammatory reaction changed in nature from an acute to a more chronic reaction. By Days 15 to 30, Group 3 still demonstrated increased signs of inflammation, including infiltration with eosinophils. The results of this study indicate that intrauterine infusion of 1% povidone-iodine solution in mares can cause chronic inflammatory changes in the endometrium. © 1992