Anti-angiogenic drug loaded liposomes: Nanotherapy for early atherosclerotic lesions in mice - Fig 4

<p>(A) Fluorescence imaging of harvested aortas. The arrows point the patchy areas where plaques are located. (B) Harvested aortas stained with Oil Red O.</p

Experimental plan designed to test the action of liposomes loaded with the anti-angiogenic agent fumagillin in early atherosclerotic lesions.

<p>Experimental plan designed to test the action of liposomes loaded with the anti-angiogenic agent fumagillin in early atherosclerotic lesions.</p

Chromatographic conditions for the analysis of fumagillin content by HPLC-UV.

<p>Chromatographic conditions for the analysis of fumagillin content by HPLC-UV.</p

Percent of aortic arch plaque obtained after treatment with angiogenesis inhibitors (LF and ILF) in mice.

<p>Plain liposomes control <b>L</b> (x), liposomes loaded with fumagillin <b>LF</b> (○), and immunoliposomes with fumagillin <b>ILF</b> (Δ) animals were treated for 5 weeks as previously described. Red line centered at each group represents median plaque area of aortic arch lesions measured in a cohort (n = 6).</p

Chemical structure of fumagillin.

<p>Chemical structure of fumagillin.</p

Percentage of lesion area relative to the area of the aortic arch.

<p>Percentage of lesion area relative to the area of the aortic arch.</p

Cross-sectional images of the aortic arch on <i>in vivo</i> T1-weighted MRI of mice treated with L and LF liposomes and obtained 24 hours after final injection.

<p>Cross-sectional images of the aortic arch on <i>in vivo</i> T1-weighted MRI of mice treated with L and LF liposomes and obtained 24 hours after final injection.</p

Representative chromatogram of the fumagillin sample obtained after liposome extraction.

<p>Fumagillin spectra presented two maximum peaks of absorbance at 335.5 and 351.1 nm. We used a wavelength of 351nm for the detection of fumagillin to avoid interferences from other liposome components. The retention time obtained was 5.35 ± 0.05 minutes (mean ± SD; n = 10).</p

Modulation of DNA Binding by Reversible Metal-Controlled Molecular Reorganizations of Scorpiand-like Ligands

DNA interaction with scorpiand azamacrocycles has been achieved through modulation of their binding affinities. Studies performed with different experimental techniques provided evidence that pH or metal-driven molecular reorganizations of these ligands regulate their ability to interact with calf thymus DNA (ctDNA) through an intercalative mode. Interestingly enough, metal-driven molecular reorganizations serve to increase or decrease the biological activities of these compounds significantly