The Influence of edaphic factors on the level of some physiological parameters for Lycium shawii in Taba Protected Area, Egypt

Abstract: The aim of this article is assess the influence of environmental factors (especially edaphic

Isolation and Biochemical Identification of Pathogenic Burkholderia Cepacia from Human Sources and Related Reactivity to Different Antibiotics

The aim of the present work is to isolate and identify Burkholderia species from different chest hospitalized patients .throat swap and sputum samples were considered. Reactivity of isolates to different antibiotics was considered using both Double Disc Synergy Test and Combined ESBL Test. Data revealed that 21/200 [10.5%] isolated from cystic fibrosis patients; samples were positive as Burkholderia isolation was more prevalent from sputum samples [55%] than throat swapping [45%]. Among the 21 bacterial isolates; 2/21(9.5%) children, 8/21(38.09%) females and 11/21(52.38%) males. The No of isolate was location related; El Sadr hospital [41%], Abbassia Chest hospital [24%], AlMattaria teaching hospital [21%] and health institute hospital [14%]. Also, isolation was gender related as male was more susceptible more than the other genders for infection by Burkholderia cepacia recording the highest value [52%] followed by female [38%] and children [10%]. Combined infection was recorded. Burkholderia cepacia was sensitive to Amikacin, Gentamicin, Kanamycin, Neomycin, Ceftazidime, Ciprofloxacin and Amoxicillin/clavulanic acid, while it were resistant to; Paromomycin, Spectinomycin, Fosfomycin, Cefixime, Levofloxacin, Ofloxacin and Cefazolin. The most active antibiotic was Amoxicillin/clavulanic acid with mean diameter of inhibition zone 38.50 mm

Phylogenetic Identification of Burkholderia Cepacia Isolates from Human Chest Infection from Different Hospitals

The genus Burkholderia includes three closely related microbial species that highlight diverse evolutionary adaptation to different niches and hosts. Burkholderia mallei are a soliped-specific pathogen that only occasionally infects humans. Burkholderia pseudomallei are a free-living soil microbe and the causative agent of the subtropical human disease melioidosis. The species that presently constitute the Burkholderia cepacia complex (Bcc) have multiple roles; they include soil and water saprophytes, rhizosphere parasites, bioremediators, plant growth promoters and plant, animal and human pathogens. Members of the Bcc are particularly associated with lifethreatening respiratory infections in patients with chronic granulomatous disease (CGD), and are the most potentially virulent, transmissible and inherently resistant microbes to have emerged as cystic fibrosis (CF) pathogens in recent decades(1;2). Burkholderia cepacia complex (Bcc) is a diverse group of 17 closely related species of the β-proteobacteria subdivision and widely distributed in natural and man-made inhabitats. In spite of advances in treatment and prevention, bacterial pathogens still pose a major threat on public health worldwide. To understand how pathogenic bacteria interact with their hosts to produce clinical disease is a fundamental issuePhylogenetic analysis of Burkholderia cepacia was achieved based on the variation of β-Lactamases production and antibacterial reactivity to different antibiotics. Sub inhibitory concentrations of augmentin down-regulates the production and/or release of exoprotein and this effect increase with increasing of the tested concentration. ESBLs was detected using double disc synergy test and combined ESBL test. Effect of reactivity of B.cepacia spp was influenced by antibiotic where Amoxicillin/ clavulanic acid showed the highest inhibitory effect and in turn clear zone dimension. Also, TEM-1 and SHV-1 genes electrophoretic pattern detected by both PFGE and RAPD. Different isolates showed a similarity to standard B.cepacia ranging from 94-95 %. Data recorded revealed the β-Lactamases genes showed a variable protein bands pattern revealing reactivity to antibiotics