EFFECT OF EFFERVESCENCE IN COMBINATION WITH SUPERDISINTEGRANTS IN THE FORMULATION OF PROPRANOLOL HCL ORAL DISINTEGRATING TABLETS

ABSTRACTObjective: The current research work is intended to formulate propranolol HCl (PLH) as orally disintegrating tablet (ODT). It is also intending to checkthe superiority in a combination of superdisintegrants and effervescent mixture than the use of superdisintegrants alone by a direct compressiontechnique. To fasten the onset of action and thereby enhancing the bioavailability of PLH in comparison to its conventional tablets.Methods: Standard calibration curve of PLH was obtained in pH 6.8 phosphate buffer by spectrophotometric method, drug-excipient compatibilitystudies were carried by Fourier transform infrared (FT-IR) studies. All the formulations were evaluated for pre and postcompression studies.Accelerated stability studies were carried out up to 6 months for the optimized formulation, EF3.Results and Discussion: Superdisintegrants used in the study are compatible with PLH. Pre- and post-compression parameters were within theacceptable limits for all formulations. In vitro dissolution kinetic studies indicate the release of PLH from ODT increases as the concentration ofsuperdisintegrants as well as the ratio of citric acid: NaHCO3 of effervescent mixture increases. Formulations with an effervescent mixture are havingrapid disintegration and dissolution rate when compared to the formulations with superdisintegrants alone. The order of superdisintegrants inenhancing the dissolution rate of PLH is crospovidone (CPV) > croscarmellose sodium (CCS) > sodium starch glycolate (SSG). Formulation, EF3 (10%CPV and 1:3, citric acid: NaHCO3 ratio, respectively) had the highest dissolution efficiency at 10 minutes (DE10=82.74%); the first order dissolutionrate constant (K1=0.141/minutes) with a regression coefficient (r2=0.974) and lesser time for 90% of drug release (t90=4 minutes), was considered asthe optimal ODT in this study. Formulation EF3, passed the test for stability.Conclusion: Hence, an effective PLH ODT was formulated by the direct compression technique with disintegration by combination of superdisintegrantsand effervescent mixture, will fasten the onset of action and enhances the bioavailability of PLH in comparison to its conventional tablets.Keywords: Propranolol HCl, Orally disintegrating tablet, Sodium starch glycolate, Croscarmellose sodium, Crospovidone, Direct compression, In vitrodissolution studies

Evaluation of rice genotypes for brown planthopper (BPH) resistance using molecular markers and phenotypic methods

Twenty eight (28) rice genotypes were used to evaluate the genetic variability based on known BPH resistant loci spread through most of the genome (chromosomes 2, 3, 4, 5, 6, 8, 10, 11 and 12), using closely linked simple sequence repeat (SSR) markers and by different phenotypic screening methods. A total number of 155 alleles were detected by 30 polymorphic markers with an average of 4.6 per locus. The genetic diversity, polymorphic information content (PIC) ranged from 0.15 to 0.89 and 0.13 to 0.88, respectively and the allele frequency ranged from 0.21 to 0.89. These microsatellite markers linked to BPH resistance loci classified rice genotypes into three clusters with additional sub groups and sub sub groups. Our study reveals high genetic variation and clear genotypic relationship for BPH resistance based on BPH resistance linked markers and known phenotypic screening methods such as standard seedbox screening technique, honey dew test and nymphal survival method. Phenotypic evaluation showed clear distinction between resistant and susceptible type by clearly revealing moderately resistant types as well. Combined use of phenotypic and genotypic evaluation methods can improve the efficiency of marker assisted selection and utilization of resistant genotypes for crop improvement by rice breeders.Keywords: Nilaparvata Lugens, microsatellite markers, polymorphism, genetic diversityAfrican Journal of Biotechnology Vol. 12(19), pp. 2515-252

Flotation of low-grade graphite ore using collector derived from low density polyethylene waste

The increasing wide range of applications of graphite for electrode, lubricants, refractory applications especially the recent surging electric automobile industry, resulting in significant need of graphite in future. Graphite demand in the energy storage industry is expected to grow 15 times faster than today's demand by 2030. Due to depleting high-grade ore, utilization of low-grade ore by beneficiation becomes utmost importance for sustainable development and resource management. In this work, low-grade graphite ore from Tamil Nadu, India with 86.84% ash was beneficiated by flotation technique for recovering graphite with lower ash content. Flotation, a surface phenomena, based on the surface hydrophobicity of the mineral surface to be separated and since graphite is naturally flotation mineral, this technique is adopted for beneficiation. The mostly commonly used collector in graphite flotation is diesel. In view of continuous cost escalation of diesel, an alternate collector was developed utilizing the low-density polyethylene (LDPE) waste paving way for plastic waste utilization. The flotation efficacy of this new collector (Collector PE) derived from LDPE waste was compared with that of diesel in graphite flotation. The run-of mine graphite ore was initially size reduced for liberation of values from its associated impurities, followed by flotation. The mesh-of-grind, dosages of collector (diesel and PE) and frother (Methyl Isobutyl Carbinol, MIBC) were optimized for better process efficiency for increasing the surface hydrophobicity of graphite particles leading to better separation efficacy. The ore characterization by x-ray diffraction revealed that graphite was accompanied predominantly by quartz with minor fractions of pyrites and several other phyllosilicates such as kaolinite and muscovite. Exfoliated morphology of graphite with thick layers were observed from SEM images. Flotation reagents such as diesel, collector PE and MIBC were characterized by FTIR to analyze their functional groups that enhances the efficiency of the separation process. A graphite float (rougher concentrate) with 15.2% weight recovery and 17.7% ash content was obtained after 10 minutes of grinding (d80: 240.5µm) with 0.85kg/t of collector (diesel) and 0.07kg/t of frother (MIBC) dosages and on two-stage cleaning, a final concentrate with 12.66% weight recovery and 8.70% ash content was obtained. A graphite final concentrate with 13.04% weight recovery and 8.90 % ash was achieved with two-stage cleaning, when treated with 0.57 kg/t of collector PE and 0.07 kg/t of MIBC. These results indicate that the flotation efficiency of the collector PE derived from LDPE wastes is comparable with that of diesel and would be economical when used in large scale industrial graphite flotation

COMPUTING CORRECT PARALLEL OF CONCEPTS LATEST KNOWLEDGE GRAPHS

We can find out all the chains by offering a unique framework for finding the best set of universe conventions, where the HUI from the Certain Utility X set is definitely a high-level theme in the database. Which is explained that the goods acquired by a software application, at least reach at least the lowest level. However, it is a difficult problem to use more accurately for users. Such types of wheat have been offered without the need to use two types of efficient algorithms to use the tank and tune-up. We discuss the discussion on their own interests and goals, and provide examples of these two algorithms. Real and artificial figures evaluation of the experiments show that the next generation of ultraviolet performance has to be given complete condition of canvas algorithms. The presentations may be successful in some special applications, these above are not the top-use elements for the fixed control, but they have also been affected by the usual problem of setting a proper set. We have a method that is applied during the birth of the UNODOD. The PPTP is extremely easy for many applications to access the punctuation model. The TKU formula is the name of the URL to maintain the elements of the elements and the utility data that adopts a tree based on an element based on an element. In the TWU model, TTU is an heir inherent and consists of two steps