15 research outputs found

    Assignment of Gene ontology (GO) term for predicted genes of <i>T</i>. <i>indica</i> genome into three categories: cellular component, molecular function and biological process.

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    <p>Assignment of Gene ontology (GO) term for predicted genes of <i>T</i>. <i>indica</i> genome into three categories: cellular component, molecular function and biological process.</p

    Comparison of the Assembly statistics of PacBio SMRT and Illumina HiSeq 2000 Sequencing of the draft genome of <i>T</i>. <i>indica</i>.

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    <p>Comparison of the Assembly statistics of PacBio SMRT and Illumina HiSeq 2000 Sequencing of the draft genome of <i>T</i>. <i>indica</i>.</p

    Synteny analysis of <i>T</i>. <i>indica</i> genome with other smut fungi, <i>U</i>. <i>maydis</i>, <i>U</i>. <i>hordei</i> and <i>S</i>. <i>reilianum</i>.

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    <p>Synteny analysis of <i>T</i>. <i>indica</i> genome with other smut fungi, <i>U</i>. <i>maydis</i>, <i>U</i>. <i>hordei</i> and <i>S</i>. <i>reilianum</i>.</p

    Phylogenetic tree showing relationship of <i>T</i>. <i>indica</i> genome with genomes of other phytopathogenic fungi.

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    <p>Maximum Likelihood tree was constructed using ITS1, 5.8S, ITS2 sequences using MEGA version 7, (Tamura 3-parameter model) with 1000 bootstraps.</p

    GPCR in different fungal genomes <sup>*</sup>.

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    <p>GPCR in different fungal genomes <sup><a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0171323#t003fn001" target="_blank">*</a></sup>.</p

    General features of the assembly statistics of hybrid assembly of draft <i>T</i>. <i>indica</i> genome.

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    <p>General features of the assembly statistics of hybrid assembly of draft <i>T</i>. <i>indica</i> genome.</p

    Evaluation of 19,460 Wheat Accessions Conserved in the Indian National Genebank to Identify New Sources of Resistance to Rust and Spot Blotch Diseases

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    <div><p>A comprehensive germplasm evaluation study of wheat accessions conserved in the Indian National Genebank was conducted to identify sources of rust and spot blotch resistance. Genebank accessions comprising three species of wheat–<i>Triticum aestivum</i>, <i>T</i>. <i>durum</i> and <i>T</i>. <i>dicoccum</i> were screened sequentially at multiple disease hotspots, during the 2011–14 crop seasons, carrying only resistant accessions to the next step of evaluation. Wheat accessions which were found to be resistant in the field were then assayed for seedling resistance and profiled using molecular markers. In the primary evaluation, 19,460 accessions were screened at Wellington (Tamil Nadu), a hotspot for wheat rusts. We identified 4925 accessions to be resistant and these were further evaluated at Gurdaspur (Punjab), a hotspot for stripe rust and at Cooch Behar (West Bengal), a hotspot for spot blotch. The second round evaluation identified 498 accessions potentially resistant to multiple rusts and 868 accessions potentially resistant to spot blotch. Evaluation of rust resistant accessions for seedling resistance against seven virulent pathotypes of three rusts under artificial epiphytotic conditions identified 137 accessions potentially resistant to multiple rusts. Molecular analysis to identify different combinations of genetic loci imparting resistance to leaf rust, stem rust, stripe rust and spot blotch using linked molecular markers, identified 45 wheat accessions containing known resistance genes against all three rusts as well as a QTL for spot blotch resistance. The resistant germplasm accessions, particularly against stripe rust, identified in this study can be excellent potential candidates to be employed for breeding resistance into the background of high yielding wheat cultivars through conventional or molecular breeding approaches, and are expected to contribute toward food security at national and global levels.</p></div

    Seedling resistance in wheat accessions.

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    <p>These genebank accessions were identified as resistant in the primary field evaluation at the hotspots. The seedling resistance was recorded as resistant either to only one rust disease (leaf, stem or stripe) or a combination of two or all the three rust diseases. Seedling resistance screening was carried out under controlled condition at the Regional Station of Indian Institute of Wheat and Barley Research (IIWBR), Flowerdale, Shimla. Accession-wise details are provided in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0167702#pone.0167702.s003" target="_blank">S3 Table</a>.</p
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