25 research outputs found

    Microseismic Activity in an Open Pit Lignite Mine

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    An increased level of horizontal stress related to tectonic forces is often held responsible for unexpectedly strong mining induced seismicity. The authors use the Belchatow open pit lignite \u27mine in central Poland to show that this seismicity can be explained without tectonic forces as well. The presented approach should offer affordable ways of detecting the problem before it occurs, and either preventing it or controlling its scale

    Sequencing, Mapping, and Analysis of 27,455 Maize Full-Length cDNAs

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    Full-length cDNA (FLcDNA) sequencing establishes the precise primary structure of individual gene transcripts. From two libraries representing 27 B73 tissues and abiotic stress treatments, 27,455 high-quality FLcDNAs were sequenced. The average transcript length was 1.44 kb including 218 bases and 321 bases of 5′ and 3′ UTR, respectively, with 8.6% of the FLcDNAs encoding predicted proteins of fewer than 100 amino acids. Approximately 94% of the FLcDNAs were stringently mapped to the maize genome. Although nearly two-thirds of this genome is composed of transposable elements (TEs), only 5.6% of the FLcDNAs contained TE sequences in coding or UTR regions. Approximately 7.2% of the FLcDNAs are putative transcription factors, suggesting that rare transcripts are well-enriched in our FLcDNA set. Protein similarity searching identified 1,737 maize transcripts not present in rice, sorghum, Arabidopsis, or poplar annotated genes. A strict FLcDNA assembly generated 24,467 non-redundant sequences, of which 88% have non-maize protein matches. The FLcDNAs were also assembled with 41,759 FLcDNAs in GenBank from other projects, where semi-strict parameters were used to identify 13,368 potentially unique non-redundant sequences from this project. The libraries, ESTs, and FLcDNA sequences produced from this project are publicly available. The annotated EST and FLcDNA assemblies are available through the maize FLcDNA web resource (www.maizecdna.org)

    Computed tomography-based spectral imaging for fluorescence microscopy.

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    The computed tomography imaging spectrometer (CTIS) is a non-scanning instrument capable of simultaneously acquiring full spectral information (450-750 nm) from every position element within its field of view (75 microm x 75 microm). The current spatial and spectral sampling intervals of the spectrometer are 1.0 microm and 10 nm, respectively. This level of resolution is adequate to resolve signal responses from multiple fluorescence probes located within individual cells or different locations within the same cell. Spectral imaging results are presented from the CTIS combined with a commercial inverted fluorescence microscope. Results demonstrate the capability of the CTIS to monitor the spatiotemporal evolution of pH in rat insulinoma cells loaded with SNARF-1. The ability to analyze full spectral information for two-dimensional (x, y) images allows precise evaluation of heterogeneous physiological responses within cell populations. Due to low signal levels, integration times up to 2 s were required. However, reasonable modifications to the instrument design will provide higher system transmission efficiency with increased temporal and spatial resolution. Specifically, a custom optical design including the use of a larger format detector array is under development for a second-generation system

    Photolithographic processing of hybrid glasses for microoptics

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    Snapshot imaging spectropolarimeter

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    Distribution UnliuLt:ýJ A revolutionary technique for snapshot imaging spectropolarimetry has been developed because of the recent availability of large focal plane arrays and fast computers. The technique involves the combination of spectropolarimetry with computed tomography imaging spectrometry (CTIS). This spectropolarimeter uses a modulation to encode the spectral dependence of all four Stokes parameters in a single spectrum. CTIS is a snapshot imaging spectrometry method in which both spatial and spectral information is reconstructed using the inverse mathematical technique of medical computed tomography. The combination of these techniques provides the basis for a snapshot imaging complete Stokes spectropolarimeter that can be implemented with no moving parts. This technique is being applied to the SWIR wavelength region to find targets that are camouflaged. 1

    Random and block copolymers based on 4-methyl-1-pentene and 1-pentene

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    The zirconium acetamidinate catalyst {Cp*Zr(Me)2[N(Et)C(Me)N(tBu)]} (Cp* = ¿5-C5Me5) was used to synthesize both random and block copolymers based on 4-methyl-1-pentene (4M1P) and 1-pentene. The polymers have been characterized by NMR spectroscopy, SEC, DSC, high temperature HPLC and CRYSTAF. Unexpectedly, the yields and molecular weights decreased with increasing amounts of 1-pentene. The reason for this behavior is that 1-pentene occasionally undergoes 2,1-misinsertions trapping the catalyst in a dormant state. These 2,1-misinsertions do not seem to occur with the bulky 4M1P (branched a-olefin). Adding a small amount of ethylene reactivates the catalyst. Unlike most semi-crystalline polymers, the density of the crystalline phase of isotactic P4M1P can be lower than of the amorphous phase, when crystallized under very high pressures. To characterize this peculiar behavior of 4M1P-based polymers, various samples have been subjected to Pressure-Volume-Temperature (PVT) measurements. While the P4M1P homopolymers and block copolymers show the expected decrease in specific volume upon crystallization, the 4M1P-rich random copolymers proved not to vary in specific volume under the same condition
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