Behaviour of two SCAR markers for seedlessness within Central European varieties of grapevine

Two working sequence-characterised amplified region markers, SCC8 and SCF27, linked to the seedless phenotype and particularly to the major locus involved in this trait, sdI, were identified in grapevine. Several seeded varieties also showed the alleles for seedlessness at these marker loci. Based on comparison of allelic distribution of two markers in a set of several seedless and seeded varieties, as well as three segregating progenies, we provide evidence that 'Chaouch rose', an ancient seeded variety of unknown origin, has potential to promote seedlessness and most probably belongs to the group of seeded varieties that harbour the sdI+ allele. We conclude that using both SCC8 and SCF27 and, in special cases, even their null alleles can help to elucidate the seedlessness of individuals that lack the amplicon accountable for seedlessness at one marker locus. However, the presence of null alleles and the genetic distance of markers from the sdI locus involved in seedlessness may cause complications.

The demonstration of the GFLV Nepovirus isolates on naturally infected grapevine cultivars and evaluation of variability within genome region encoding movement protein

In this work, the infection of Grapevine fanleaf virus (GFLV) disease was described on the base of symptomatic differences within eight grapevine plants of six grape ciltivars with positive tests on GFLV. Among them, cultivars Kodrjanka, Pamjati Negrula, Kišmiš Lučistyj were planted in wine region of the South Moravia (Czech Republic), three infected grapevine cultivars (URS, Cinsaut, Dimrit) included in this study originating from Italy. Except symptomatic evaluation, the differences between isolates were emphasized at the genetic level too, exactly in the frame of RNA2 genomic region coding movement protein. The variability of the tested isolates within the eight plants was in the range from 86.59 to 97.61% at the nucleotide level. The results confirmed very high degree of similarity between virus isolates of GFLV within studied RNA2 region. This fact was assessed by the phylogenetic analysis of obtained sequencing data too