Effect of caffeine -coconut products interactions on induction of microsomal drug-metabolizing enzymes in wistar albino rats

Effect of caffeine-coconut products interactions on induction of drug-metabolizing enzyme in wistar albino rats was studied. Twenty rats were randomly divided into four groups: The control group (1) received via oral route a placebo (4.0ml of distilled water). Groups 2 to 4 were treated for a 14-day period with 50mg/kg body weight of caffeine, 50mg/kg body weight of caffeine and 50mg/kg body weight of coconut water, and 50mg/kg body weight of caffeine and 50mg/kg body weight ofcoconut milk in 4.0ml of the vehicle via gastric intubation respectively. One day after the final exposure, the animals were anaestheticized by inhalation of an overdose of chloroform. The blood of each rat was collected by cardiac puncture while the liver of each rat was harvested and processed to examine several biochemical parameters, ie, total protein and RNA levels, protein/RNA ratios, and activities of alanine and aspartate amino transferase (ALT and AST, respectively). The results showed that while ingestion of coconut milk and coconut water increased the values of protein and protein/RNA ratios, it decreased alanine and aspartate amino transferase (ALT and AST) activities.These effects, in turn, enhanced the induction of the metabolizing enzymes and a resultant faster clearance and elimination of the caffeine from the body, there by reducing the toxic effect on the liver

Effect of alcohol and kolanut interaction on brain sodium pump activity in wistar albino rats.

Effect of alcohol - kolanut interaction on Sodium Pump activity in wistar albino rats was studied. Thirty wistar albino rats were divided into six groups of five (5) rats per group and used for the study. The control group (1) received via oral route a placebo (4ml of distilled water). Groups 2 to 6 were treated for a period of 21 days, with (10% v/v) of alcohol (group 2), 50mg/kg body weight of kolanut (group 3), 50mg/kg body weight of caffeine (group 4), 4ml of 10% v/v of alcohol and50mg/kg body weight kolanut (group 5), 4ml of 10% v/v of alcohol and 50mg/kg body weight of caffeine in 4.0ml of the vehicle via gastric intubation respectively. A day after the final exposure, thebrain of each rat was harvested and processed to examine several biochemical parameters, i.e., total ATpase, ouabain-insensitive ATpase, ouabain sensitive ATpase (Na+ - K+ - ATpase), non-enzymatic breakdown of ATP and inorganic phosphate (Pi) released. The results showed that the essential enzyme of the brain responsible for neuronal function, Na+ - K+ - ATpase, was inhibited by alcohol-kolanut coadministration relative to control, resulting in a decrease in Na+ - K+ - ATpase activity, ATP production,ion transport and action potential, leading to loss of neuronal activities

Biochemical Alterations In Colletotrichum lindemuthianum Infected Pumpkin (Cucurbita pepo) Leaf.

A leaf spot disease of pumpkin (Cucurbita pepo) was observed in farms in the 2004 cropping season in Cross River State, Nigeria. Isolation, identification and pathogenicity trials carried out on the causative agent, implicated the fungus Colletotrichum lindemuthianum. Analysis carried out, revealed that apart from fat (0%), there were alterations in other biochemical contents of the infected host plant. There was 8.84% increase in moisture content of the infected sample, 8.45% increase in protein, and 47.06% increase in ash content over the healthy sample. Reductions in fibre (-6.74%) and carbohydrate (-8.12%) contents were recorded for the diseased host leaves. From the results, it is recommended that control measures be applied by late March or early April before early rains. Environmentally friendly approach like medicinal plants extracts application is very highly recommended. Data collection and analysis was carried out at 1 month post inoculation (1MPI)

Effect Of Microbial Fermentation Of Lignocellulose Wastes On Nutritive Status

Lignocellulosic wastes (municipal wastes and cassava peels) were hydrolyzed with enzymes (cellulase and glycosidase) and fermented for ethanol using saccharamyce cerevisae. The amount of ethanol produced was found to be both substrate and concentration dependent. Higher yields of ethanol were obtained with less crystalline wastes. Higher yields of ethanol were obtained with cassava peels than with municipal wastes. In both case the best yields of ethanol were in the presence of glucose and acid-hydrolyzed starch. In both cases, the fermented wastes gave higher protein content than the unfermented wastes. The possibility of employing the fermented products food and animal feed supplement is suggested. The feasibility of employing the cassava peels and municipal wastes as sources of ethanol or alcoholic beverages is also worth consideration. Keywords: Lignocelluloses wastes, municipal wastes, cassava peels, saccharamyce cerevisae and ethanol.Global Journal or Pure and Applied Sciences Vol. 14 (4) 2008: pp.379-38

EFFECT OF CAFFEINE -COCONUT PRODUCTS INTERACTIONS ON INDUCTION OF MICROSOMAL DRUG-METABOLIZING ENZYMES IN WISTAR ALBINO RATS

Effect of caffeine-coconut products interactions on induction of drug-metabolizing enzyme in wistar albino rats was studied. Twenty rats were randomly divided into four groups: The control group (1) received via oral route a placebo (4.0ml of distilled water). Groups 2 to 4 were treated for a 14-day period with 50mg/kg body weight of caffeine, 50mg/kg body weight of caffeine and 50mg/kg body weight of coconut water, and 50mg/kg body weight of caffeine and 50mg/kg body weight of coconut milk in 4.0ml of the vehicle via gastric intubation respectively. One day after the final exposure, the animals were anaestheticized by inhalation of an overdose of chloroform. The blood of each rat was collected by cardiac puncture while the liver of each rat was harvested and processed to examine several biochemical parameters, ie, total protein and RNA levels, protein/RNA ratios, and activities of alanine and aspartate amino transferase (ALT and AST, respectively). The results showed that while ingestion of coconut milk and coconut water increased the values of protein and protein/RNA ratios, it decreased alanine and aspartate amino transferase (ALT and AST) activities. These effects, in turn, enhanced the induction of the metabolizing enzymes and a resultant faster clearance and elimination of the caffeine from the body, there by reducing the toxic effect on the liver

EFFECT OF ALCOHOL AND KOLANUT INTERACTION ON BRAIN SODIUM PUMP ACTIVITY IN WISTAR ALBINO RATS.

Effect of alcohol - kolanut interaction on Sodium Pump activity in wistar albino rats was studied. Thirty wistar albino rats were divided into six groups of five (5) rats per group and used for the study. The control group (1) received via oral route a placebo (4ml of distilled water). Groups 2 to 6 were treated for a period of 21 days, with (10% v/v) of alcohol (group 2), 50mg/kg body weight of kolanut (group 3), 50mg/kg body weight of caffeine (group 4), 4ml of 10% v/v of alcohol and 50mg/kg body weight kolanut (group 5), 4ml of 10% v/v of alcohol and 50mg/kg body weight of caffeine in 4.0ml of the vehicle via gastric intubation respectively. A day after the final exposure, the brain of each rat was harvested and processed to examine several biochemical parameters, i.e., total ATpase, ouabain-insensitive ATpase, ouabain sensitive ATpase (Na+ - K+- ATpase), non-enzymatic breakdown of ATP and inorganic phosphate (Pi) released. The results showed that the essential enzyme of the brain responsible for neuronal function, Na+ - K+- ATpase, was inhibited by alcohol-kolanut co-administration relative to control, resulting in a decrease in Na+ - K+- ATpase activity, ATP production, ion transport and action potential, leading to loss of neuronal activities

Effect of alcohol and kolanut-co-administration on serum and brain cholesterol and phospholipid levels in wistar albino rats

No Abstract.GJPAS Vol. 13 (4) 2007: pp. 537-53